Alginate-Capped Silver Nanoparticles as a Potent Anti-mycobacterial Agent Against Mycobacterium tuberculosis
Tuberculosis (TB) is a leading cause of death from a single infectious agent, Mycobacterium tuberculosis (Mtb). Although progress has been made in TB control, still about 10 million people worldwide develop TB annually and 1.5 million die of the disease. The rapid emergence of aggressive, drug-resis...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-11-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2021.746496/full |
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| author | Cheng-Cheung Chen Cheng-Cheung Chen Yih-Yuan Chen Chang-Ching Yeh Chia-Wei Hsu Shang-Jie Yu Chih-Hao Hsu Ting-Chun Wei Sin-Ni Ho Pei-Chu Tsai Yung-Deng Song Hui-Ju Yen Hui-Ju Yen Xin-An Chen Jenn-Jong Young Chuan-Chung Chuang Chuan-Chung Chuang Horng-Yunn Dou Horng-Yunn Dou |
| author_facet | Cheng-Cheung Chen Cheng-Cheung Chen Yih-Yuan Chen Chang-Ching Yeh Chia-Wei Hsu Shang-Jie Yu Chih-Hao Hsu Ting-Chun Wei Sin-Ni Ho Pei-Chu Tsai Yung-Deng Song Hui-Ju Yen Hui-Ju Yen Xin-An Chen Jenn-Jong Young Chuan-Chung Chuang Chuan-Chung Chuang Horng-Yunn Dou Horng-Yunn Dou |
| author_sort | Cheng-Cheung Chen |
| collection | DOAJ |
| description | Tuberculosis (TB) is a leading cause of death from a single infectious agent, Mycobacterium tuberculosis (Mtb). Although progress has been made in TB control, still about 10 million people worldwide develop TB annually and 1.5 million die of the disease. The rapid emergence of aggressive, drug-resistant strains and latent infections have caused TB to remain a global health challenge. TB treatments are lengthy and their side effects lead to poor patient compliance, which in turn has contributed to the drug resistance and exacerbated the TB epidemic. The relatively low output of newly approved antibiotics has spurred research interest toward alternative antibacterial molecules such as silver nanoparticles (AgNPs). In the present study, we use the natural biopolymer alginate to serve as a stabilizer and/or reductant to green synthesize AgNPs, which improves their biocompatibility and avoids the use of toxic chemicals. The average size of the alginate-capped AgNPs (ALG-AgNPs) was characterized as nanoscale, and the particles were round in shape. Drug susceptibility tests showed that these ALG-AgNPs are effective against both drug-resistant Mtb strains and dormant Mtb. A bacterial cell-wall permeability assay showed that the anti-mycobacterial action of ALG-AgNPs is mediated through an increase in cell-wall permeability. Notably, the anti-mycobacterial potential of ALG-AgNPs was effective in both zebrafish and mouse TB animal models in vivo. These results suggest that ALG-AgNPs could provide a new therapeutic option to overcome the difficulties of current TB treatments. |
| first_indexed | 2024-04-11T20:49:13Z |
| format | Article |
| id | doaj.art-8cbd6e4b0df74eb685a41fc17de84b10 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-11T20:49:13Z |
| publishDate | 2021-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-8cbd6e4b0df74eb685a41fc17de84b102022-12-22T04:03:55ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122021-11-011210.3389/fphar.2021.746496746496Alginate-Capped Silver Nanoparticles as a Potent Anti-mycobacterial Agent Against Mycobacterium tuberculosisCheng-Cheung Chen0Cheng-Cheung Chen1Yih-Yuan Chen2Chang-Ching Yeh3Chia-Wei Hsu4Shang-Jie Yu5Chih-Hao Hsu6Ting-Chun Wei7Sin-Ni Ho8Pei-Chu Tsai9Yung-Deng Song10Hui-Ju Yen11Hui-Ju Yen12Xin-An Chen13Jenn-Jong Young14Chuan-Chung Chuang15Chuan-Chung Chuang16Horng-Yunn Dou17Horng-Yunn Dou18Institute of Preventive Medicine, National Defense Medical Center, Taipei, TaiwanGraduate Institute of Medical Science, National Defense Medical Center, Taipei, TaiwanDepartment of Biochemical Science and Technology, National Chiayi University, Chia-Yi, TaiwanNational Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, TaiwanNational Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, TaiwanNational Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, TaiwanNational Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, TaiwanNational Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, TaiwanNational Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, TaiwanNational Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, TaiwanNational Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, TaiwanInstitute of Preventive Medicine, National Defense Medical Center, Taipei, TaiwanSchool of Pharmacy, National Defense Medical Center, Taipei, TaiwanInstitute of Preventive Medicine, National Defense Medical Center, Taipei, TaiwanInstitute of Preventive Medicine, National Defense Medical Center, Taipei, TaiwanSchool of Dentistry and Graduate Institute of Dental Science, National Defense Medical Center, Taipei, TaiwanDepartment of Dentistry, Tri-Service General Hospital, Taipei, TaiwanNational Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, TaiwanDepartment of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, TaiwanTuberculosis (TB) is a leading cause of death from a single infectious agent, Mycobacterium tuberculosis (Mtb). Although progress has been made in TB control, still about 10 million people worldwide develop TB annually and 1.5 million die of the disease. The rapid emergence of aggressive, drug-resistant strains and latent infections have caused TB to remain a global health challenge. TB treatments are lengthy and their side effects lead to poor patient compliance, which in turn has contributed to the drug resistance and exacerbated the TB epidemic. The relatively low output of newly approved antibiotics has spurred research interest toward alternative antibacterial molecules such as silver nanoparticles (AgNPs). In the present study, we use the natural biopolymer alginate to serve as a stabilizer and/or reductant to green synthesize AgNPs, which improves their biocompatibility and avoids the use of toxic chemicals. The average size of the alginate-capped AgNPs (ALG-AgNPs) was characterized as nanoscale, and the particles were round in shape. Drug susceptibility tests showed that these ALG-AgNPs are effective against both drug-resistant Mtb strains and dormant Mtb. A bacterial cell-wall permeability assay showed that the anti-mycobacterial action of ALG-AgNPs is mediated through an increase in cell-wall permeability. Notably, the anti-mycobacterial potential of ALG-AgNPs was effective in both zebrafish and mouse TB animal models in vivo. These results suggest that ALG-AgNPs could provide a new therapeutic option to overcome the difficulties of current TB treatments.https://www.frontiersin.org/articles/10.3389/fphar.2021.746496/fullmycobacterium tubeculosisantimycobacterial agentMDR TBdormant TBsilver nanopaiticles |
| spellingShingle | Cheng-Cheung Chen Cheng-Cheung Chen Yih-Yuan Chen Chang-Ching Yeh Chia-Wei Hsu Shang-Jie Yu Chih-Hao Hsu Ting-Chun Wei Sin-Ni Ho Pei-Chu Tsai Yung-Deng Song Hui-Ju Yen Hui-Ju Yen Xin-An Chen Jenn-Jong Young Chuan-Chung Chuang Chuan-Chung Chuang Horng-Yunn Dou Horng-Yunn Dou Alginate-Capped Silver Nanoparticles as a Potent Anti-mycobacterial Agent Against Mycobacterium tuberculosis Frontiers in Pharmacology mycobacterium tubeculosis antimycobacterial agent MDR TB dormant TB silver nanopaiticles |
| title | Alginate-Capped Silver Nanoparticles as a Potent Anti-mycobacterial Agent Against Mycobacterium tuberculosis |
| title_full | Alginate-Capped Silver Nanoparticles as a Potent Anti-mycobacterial Agent Against Mycobacterium tuberculosis |
| title_fullStr | Alginate-Capped Silver Nanoparticles as a Potent Anti-mycobacterial Agent Against Mycobacterium tuberculosis |
| title_full_unstemmed | Alginate-Capped Silver Nanoparticles as a Potent Anti-mycobacterial Agent Against Mycobacterium tuberculosis |
| title_short | Alginate-Capped Silver Nanoparticles as a Potent Anti-mycobacterial Agent Against Mycobacterium tuberculosis |
| title_sort | alginate capped silver nanoparticles as a potent anti mycobacterial agent against mycobacterium tuberculosis |
| topic | mycobacterium tubeculosis antimycobacterial agent MDR TB dormant TB silver nanopaiticles |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2021.746496/full |
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