Analysis of main T-cell subsets and activated T supressor/cytotoxic cells in patients with Borrelia burgdorferi s. lato only infection and co-infections with Anaplasma phagocytophilum , Bartonella spp. and Babesia microti
Introduction The study was designed to assess the role of some important immunologic factors with regards to both laboratory results and clinical symptoms in patients with confirmed Lyme disease. Additional examinations were carried out for co-infections with a number of tick-borne pathogens. Mater...
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| Format: | Article |
| Language: | English |
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Institute of Rural Health
2015-12-01
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| Series: | Annals of Agricultural and Environmental Medicine |
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| Online Access: | http://www.journalssystem.com/aaem/Analysis-of-main-T-cell-subsets-and-activated-T-supressor-cytotoxic-cells-in-patients-with-i-Borrelia-burgdorferi-i-s-lato-only-infection-and-co-infections-with-i-Anaplasma-phagocytophilum-i-i-Bartonella-i-spp-and-i-Babesia-microti-i-,72385,0,2.html |
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| author | Krzysztof Tomasiewicz Jolanta Chmielewska-Badora Jacek Zwolinski Elzbieta Murias-Brylowska |
| author_facet | Krzysztof Tomasiewicz Jolanta Chmielewska-Badora Jacek Zwolinski Elzbieta Murias-Brylowska |
| author_sort | Krzysztof Tomasiewicz |
| collection | DOAJ |
| description | Introduction
The study was designed to assess the role of some important immunologic factors with regards to both laboratory results and clinical symptoms in patients with confirmed Lyme disease. Additional examinations were carried out for co-infections with a number of tick-borne pathogens.
Material and Methods
The study group consisted of 54 patients with Lyme disease and a group of 21 healthy controls. Serology of co-infections with Anaplasma phagocytophilum , Bartonella spp. and Babesia microti was carrieed out in all patients. Blood samples were stained using the whole-blood lysis method and analyzed concurrently on a flow cytometer FACSCalibur. Directly conjugated anti-human monoclonal antibodies against CD3, CD4, CD8, CD16, CD56, HLA-DR and CD69 were used.
Results
No significant differences were observed with respect to thepretreatment level of CD4+ and CD8+ cells. In patients with symptoms relief and symptoms persistence, lower percentages of CD4+ and CD8+ cells were found, but with no statistical dependence. In the study group, both in patients with and without co-infections, pretreatment values of CD16+CD56+ cells did not differ significantly. In patients who did not respond to the treatment, the baseline percentage of NK cells was higher (P<0.01) than in group with clinical improvement, and lower after the treatment, whereas in patients with symptoms relief after the treatment there was an increase in the percentage of NK cells.
Conclusions
Co-infections with Anaplasma phagocytophilum , Bartonella spp. and Babesia microti had no impact on T-cell percentages in Lyme disease patients. There was a lower baseline percentage of NK cells in patients not responding to antibiotic treatment. |
| first_indexed | 2024-12-11T21:03:13Z |
| format | Article |
| id | doaj.art-8cbda6da748a49c68d037cad405c40ea |
| institution | Directory Open Access Journal |
| issn | 1232-1966 1898-2263 |
| language | English |
| last_indexed | 2024-12-11T21:03:13Z |
| publishDate | 2015-12-01 |
| publisher | Institute of Rural Health |
| record_format | Article |
| series | Annals of Agricultural and Environmental Medicine |
| spelling | doaj.art-8cbda6da748a49c68d037cad405c40ea2022-12-22T00:50:56ZengInstitute of Rural HealthAnnals of Agricultural and Environmental Medicine1232-19661898-22632015-12-0123111111510.5604/12321966.119686472385Analysis of main T-cell subsets and activated T supressor/cytotoxic cells in patients with Borrelia burgdorferi s. lato only infection and co-infections with Anaplasma phagocytophilum , Bartonella spp. and Babesia microtiKrzysztof Tomasiewicz0Jolanta Chmielewska-Badora1Jacek Zwolinski2Elzbieta Murias-Brylowska3Department of Infectious Diseases, Medical University of Lublin, PolandDepartment of Allergology and Environmental Hazards, Institute of Rural Health, Lublin, PolandDepartment of Allergology and Environmental Hazards, Institute of Rural Health, Lublin, PolandDepartment of Infectious Diseases, Medical University of Lublin, PolandIntroduction The study was designed to assess the role of some important immunologic factors with regards to both laboratory results and clinical symptoms in patients with confirmed Lyme disease. Additional examinations were carried out for co-infections with a number of tick-borne pathogens. Material and Methods The study group consisted of 54 patients with Lyme disease and a group of 21 healthy controls. Serology of co-infections with Anaplasma phagocytophilum , Bartonella spp. and Babesia microti was carrieed out in all patients. Blood samples were stained using the whole-blood lysis method and analyzed concurrently on a flow cytometer FACSCalibur. Directly conjugated anti-human monoclonal antibodies against CD3, CD4, CD8, CD16, CD56, HLA-DR and CD69 were used. Results No significant differences were observed with respect to thepretreatment level of CD4+ and CD8+ cells. In patients with symptoms relief and symptoms persistence, lower percentages of CD4+ and CD8+ cells were found, but with no statistical dependence. In the study group, both in patients with and without co-infections, pretreatment values of CD16+CD56+ cells did not differ significantly. In patients who did not respond to the treatment, the baseline percentage of NK cells was higher (P<0.01) than in group with clinical improvement, and lower after the treatment, whereas in patients with symptoms relief after the treatment there was an increase in the percentage of NK cells. Conclusions Co-infections with Anaplasma phagocytophilum , Bartonella spp. and Babesia microti had no impact on T-cell percentages in Lyme disease patients. There was a lower baseline percentage of NK cells in patients not responding to antibiotic treatment.http://www.journalssystem.com/aaem/Analysis-of-main-T-cell-subsets-and-activated-T-supressor-cytotoxic-cells-in-patients-with-i-Borrelia-burgdorferi-i-s-lato-only-infection-and-co-infections-with-i-Anaplasma-phagocytophilum-i-i-Bartonella-i-spp-and-i-Babesia-microti-i-,72385,0,2.htmlLyme diseaseAnaplasma phagocytophilumBartonellaBabesia microtiimmunologyT-cell subsetstherapy |
| spellingShingle | Krzysztof Tomasiewicz Jolanta Chmielewska-Badora Jacek Zwolinski Elzbieta Murias-Brylowska Analysis of main T-cell subsets and activated T supressor/cytotoxic cells in patients with Borrelia burgdorferi s. lato only infection and co-infections with Anaplasma phagocytophilum , Bartonella spp. and Babesia microti Annals of Agricultural and Environmental Medicine Lyme disease Anaplasma phagocytophilum Bartonella Babesia microti immunology T-cell subsets therapy |
| title | Analysis of main T-cell subsets and activated T supressor/cytotoxic cells in patients with
Borrelia burgdorferi s. lato only infection and co-infections with Anaplasma phagocytophilum , Bartonella spp. and Babesia microti |
| title_full | Analysis of main T-cell subsets and activated T supressor/cytotoxic cells in patients with
Borrelia burgdorferi s. lato only infection and co-infections with Anaplasma phagocytophilum , Bartonella spp. and Babesia microti |
| title_fullStr | Analysis of main T-cell subsets and activated T supressor/cytotoxic cells in patients with
Borrelia burgdorferi s. lato only infection and co-infections with Anaplasma phagocytophilum , Bartonella spp. and Babesia microti |
| title_full_unstemmed | Analysis of main T-cell subsets and activated T supressor/cytotoxic cells in patients with
Borrelia burgdorferi s. lato only infection and co-infections with Anaplasma phagocytophilum , Bartonella spp. and Babesia microti |
| title_short | Analysis of main T-cell subsets and activated T supressor/cytotoxic cells in patients with
Borrelia burgdorferi s. lato only infection and co-infections with Anaplasma phagocytophilum , Bartonella spp. and Babesia microti |
| title_sort | analysis of main t cell subsets and activated t supressor cytotoxic cells in patients with borrelia burgdorferi s lato only infection and co infections with anaplasma phagocytophilum bartonella spp and babesia microti |
| topic | Lyme disease Anaplasma phagocytophilum Bartonella Babesia microti immunology T-cell subsets therapy |
| url | http://www.journalssystem.com/aaem/Analysis-of-main-T-cell-subsets-and-activated-T-supressor-cytotoxic-cells-in-patients-with-i-Borrelia-burgdorferi-i-s-lato-only-infection-and-co-infections-with-i-Anaplasma-phagocytophilum-i-i-Bartonella-i-spp-and-i-Babesia-microti-i-,72385,0,2.html |
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