Antigenic Characterization of Infectious Bronchitis Virus in the South China during 2021–2022
Avian infectious bronchitis is a serious and highly contagious disease that is caused by the infectious bronchitis virus (IBV). From January 2021 to June 2022, 1008 chicken tissue samples were collected from various regions of southern China, and 15 strains of the IBV were isolated. Phylogenetic ana...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-05-01
|
| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/15/6/1273 |
| _version_ | 1797592376065130496 |
|---|---|
| author | Weifeng Yuan Ting Lv Weiwei Jiang Yuechi Hou Qingyi Wang Jinlian Ren Lei Fan Bin Xiang Qiuyan Lin Chan Ding Tao Ren Libin Chen |
| author_facet | Weifeng Yuan Ting Lv Weiwei Jiang Yuechi Hou Qingyi Wang Jinlian Ren Lei Fan Bin Xiang Qiuyan Lin Chan Ding Tao Ren Libin Chen |
| author_sort | Weifeng Yuan |
| collection | DOAJ |
| description | Avian infectious bronchitis is a serious and highly contagious disease that is caused by the infectious bronchitis virus (IBV). From January 2021 to June 2022, 1008 chicken tissue samples were collected from various regions of southern China, and 15 strains of the IBV were isolated. Phylogenetic analysis revealed that the strains mainly comprised the QX type, belonging to the same genotype as the currently prevalent LX4 type, and identified four recombination events in the S1 gene, among which lineages GI-13 and GI-19 were most frequently involved in recombination. Further study of seven selected isolates revealed that they caused respiratory symptoms, including coughing, sneezing, nasal discharge, and tracheal sounds, accompanied by depression. Inoculation of chicken embryos with the seven isolates resulted in symptoms such as curling, weakness, and bleeding. Immunization of specific pathogen-free (SPF) chickens with inactivated isolates produced high antibody levels that neutralized the corresponding strains; however, antibodies produced by vaccine strains were not effective in neutralizing the isolates. No unambiguous association was found between IBV genotypes and serotypes. In summary, a new trend in IBV prevalence has emerged in southern China, and currently available vaccines do not provide protection against the prevalent IBV strains in this region, facilitating the continued spread of IBV. |
| first_indexed | 2024-03-11T01:50:11Z |
| format | Article |
| id | doaj.art-8cbfd99bae6849c482bc716d0e029a23 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-11T01:50:11Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-8cbfd99bae6849c482bc716d0e029a232023-11-18T13:01:26ZengMDPI AGViruses1999-49152023-05-01156127310.3390/v15061273Antigenic Characterization of Infectious Bronchitis Virus in the South China during 2021–2022Weifeng Yuan0Ting Lv1Weiwei Jiang2Yuechi Hou3Qingyi Wang4Jinlian Ren5Lei Fan6Bin Xiang7Qiuyan Lin8Chan Ding9Tao Ren10Libin Chen11College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaCollege of Veterinary Medicine, Yunnan Agricultural University, Kunming 650201, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaShanghai Veterinary Research Institute (SHVRI), Chinese Academy of Agricultural Sciences (CAAS), Shanghai 200241, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaAvian infectious bronchitis is a serious and highly contagious disease that is caused by the infectious bronchitis virus (IBV). From January 2021 to June 2022, 1008 chicken tissue samples were collected from various regions of southern China, and 15 strains of the IBV were isolated. Phylogenetic analysis revealed that the strains mainly comprised the QX type, belonging to the same genotype as the currently prevalent LX4 type, and identified four recombination events in the S1 gene, among which lineages GI-13 and GI-19 were most frequently involved in recombination. Further study of seven selected isolates revealed that they caused respiratory symptoms, including coughing, sneezing, nasal discharge, and tracheal sounds, accompanied by depression. Inoculation of chicken embryos with the seven isolates resulted in symptoms such as curling, weakness, and bleeding. Immunization of specific pathogen-free (SPF) chickens with inactivated isolates produced high antibody levels that neutralized the corresponding strains; however, antibodies produced by vaccine strains were not effective in neutralizing the isolates. No unambiguous association was found between IBV genotypes and serotypes. In summary, a new trend in IBV prevalence has emerged in southern China, and currently available vaccines do not provide protection against the prevalent IBV strains in this region, facilitating the continued spread of IBV.https://www.mdpi.com/1999-4915/15/6/1273infectious bronchitis viruschicken coronavirusantigenic characterizationmolecular evolution |
| spellingShingle | Weifeng Yuan Ting Lv Weiwei Jiang Yuechi Hou Qingyi Wang Jinlian Ren Lei Fan Bin Xiang Qiuyan Lin Chan Ding Tao Ren Libin Chen Antigenic Characterization of Infectious Bronchitis Virus in the South China during 2021–2022 Viruses infectious bronchitis virus chicken coronavirus antigenic characterization molecular evolution |
| title | Antigenic Characterization of Infectious Bronchitis Virus in the South China during 2021–2022 |
| title_full | Antigenic Characterization of Infectious Bronchitis Virus in the South China during 2021–2022 |
| title_fullStr | Antigenic Characterization of Infectious Bronchitis Virus in the South China during 2021–2022 |
| title_full_unstemmed | Antigenic Characterization of Infectious Bronchitis Virus in the South China during 2021–2022 |
| title_short | Antigenic Characterization of Infectious Bronchitis Virus in the South China during 2021–2022 |
| title_sort | antigenic characterization of infectious bronchitis virus in the south china during 2021 2022 |
| topic | infectious bronchitis virus chicken coronavirus antigenic characterization molecular evolution |
| url | https://www.mdpi.com/1999-4915/15/6/1273 |
| work_keys_str_mv | AT weifengyuan antigeniccharacterizationofinfectiousbronchitisvirusinthesouthchinaduring20212022 AT tinglv antigeniccharacterizationofinfectiousbronchitisvirusinthesouthchinaduring20212022 AT weiweijiang antigeniccharacterizationofinfectiousbronchitisvirusinthesouthchinaduring20212022 AT yuechihou antigeniccharacterizationofinfectiousbronchitisvirusinthesouthchinaduring20212022 AT qingyiwang antigeniccharacterizationofinfectiousbronchitisvirusinthesouthchinaduring20212022 AT jinlianren antigeniccharacterizationofinfectiousbronchitisvirusinthesouthchinaduring20212022 AT leifan antigeniccharacterizationofinfectiousbronchitisvirusinthesouthchinaduring20212022 AT binxiang antigeniccharacterizationofinfectiousbronchitisvirusinthesouthchinaduring20212022 AT qiuyanlin antigeniccharacterizationofinfectiousbronchitisvirusinthesouthchinaduring20212022 AT chanding antigeniccharacterizationofinfectiousbronchitisvirusinthesouthchinaduring20212022 AT taoren antigeniccharacterizationofinfectiousbronchitisvirusinthesouthchinaduring20212022 AT libinchen antigeniccharacterizationofinfectiousbronchitisvirusinthesouthchinaduring20212022 |