Assessment of the Microbial Communities in Soil Contaminated with Petroleum Using Next-Generation Sequencing Tools
Microbial communities are known to play a principal role in petroleum degradation. This study tries to determine the composition of bacteria in selected crude oil-contaminated soil from Tabasco and Tamaulipas states, Mexico. We determined the microbial populations living under these conditions. We e...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-06-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/13/12/6922 |
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| author | Raul García-García Virgilio Bocanegra-García Lourdes Vital-López Jaime García-Mena Marco Antonio Zamora-Antuñano María Antonia Cruz-Hernández Juvenal Rodríguez-Reséndiz Alberto Mendoza-Herrera |
| author_facet | Raul García-García Virgilio Bocanegra-García Lourdes Vital-López Jaime García-Mena Marco Antonio Zamora-Antuñano María Antonia Cruz-Hernández Juvenal Rodríguez-Reséndiz Alberto Mendoza-Herrera |
| author_sort | Raul García-García |
| collection | DOAJ |
| description | Microbial communities are known to play a principal role in petroleum degradation. This study tries to determine the composition of bacteria in selected crude oil-contaminated soil from Tabasco and Tamaulipas states, Mexico. We determined the microbial populations living under these conditions. We evaluated the structure and diversity of bacterial communities in the contaminated soil samples. The most abundant phylum is proteobacteria. Next Generation Sequencing (NGS) analysis of the sampled soils from both states revealed that this phylum has the most relative abundance among the identified bacteria phyla. The heatmap represented the relative percentage of each genus within each sample and clustered the four samples into two groups. Moreover, this allowed us to identify many genera in alkaline soil from Tamaulipas, such as <i>Skermanella</i> sp., <i>Azospirillum</i> sp. and Unclassified species from the Rhodospirillaceae family in higher abundance. Meanwhile, in acidic soil from Tabasco, we identified <i>Thalassospira</i>, Unclassified members of the Sphingomonadaceae family and Unclassified members of the Alphaproteobacteria class with higher abundance. Alpha diversity analysis showed a low diversity (Shannon and Simpson index); Chao observed species in both Regions. These results suggest that the bacteria identified in these genera may possess the ability to degrade petroleum, and further studies in the future should elucidate their role in petroleum degradation. |
| first_indexed | 2024-03-11T02:48:51Z |
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| id | doaj.art-8cbfec8db8d849d39adbeebebfb6b424 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-11T02:48:51Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-8cbfec8db8d849d39adbeebebfb6b4242023-11-18T09:06:26ZengMDPI AGApplied Sciences2076-34172023-06-011312692210.3390/app13126922Assessment of the Microbial Communities in Soil Contaminated with Petroleum Using Next-Generation Sequencing ToolsRaul García-García0Virgilio Bocanegra-García1Lourdes Vital-López2Jaime García-Mena3Marco Antonio Zamora-Antuñano4María Antonia Cruz-Hernández5Juvenal Rodríguez-Reséndiz6Alberto Mendoza-Herrera7Division of Chemistry and Renewable Energy, Universidad Tecnologica de San Juan del Rio (UTSJR), San Juan del Rio 76900, Queretaro, MexicoLaboratorio Interacción Ambiente-Microorganismo, Instituto Politécnico Nacional, Centro de Biotecnología Genómica, Reynosa 88710, Tamaulipas, MexicoCarrera de Mantenimiento Industrial, Universidad Tecnológica de Tamaulipas Norte, Reynosa 88680, Tamaulipas, MexicoDepartment of Genetics and Molecular Biology, Cinvestav, Av. IPN# 2508, Col. Zacatenco, Mexico City 07360, MexicoEngineering Area and Centro de Investigación, Innovación y Desarrollo Tecnológico de UVM (CIIDETEC-UVM), Universidad del Valle de Mexico (UVM), Santiago de Queretaro 76230, Queretaro, MexicoLaboratorio Interacción Ambiente-Microorganismo, Instituto Politécnico Nacional, Centro de Biotecnología Genómica, Reynosa 88710, Tamaulipas, MexicoFacultad de Ingeniería, Universidad Autónoma de Querétaro, Querétaro 76010, Querétaro, MexicoLaboratorio Interacción Ambiente-Microorganismo, Instituto Politécnico Nacional, Centro de Biotecnología Genómica, Reynosa 88710, Tamaulipas, MexicoMicrobial communities are known to play a principal role in petroleum degradation. This study tries to determine the composition of bacteria in selected crude oil-contaminated soil from Tabasco and Tamaulipas states, Mexico. We determined the microbial populations living under these conditions. We evaluated the structure and diversity of bacterial communities in the contaminated soil samples. The most abundant phylum is proteobacteria. Next Generation Sequencing (NGS) analysis of the sampled soils from both states revealed that this phylum has the most relative abundance among the identified bacteria phyla. The heatmap represented the relative percentage of each genus within each sample and clustered the four samples into two groups. Moreover, this allowed us to identify many genera in alkaline soil from Tamaulipas, such as <i>Skermanella</i> sp., <i>Azospirillum</i> sp. and Unclassified species from the Rhodospirillaceae family in higher abundance. Meanwhile, in acidic soil from Tabasco, we identified <i>Thalassospira</i>, Unclassified members of the Sphingomonadaceae family and Unclassified members of the Alphaproteobacteria class with higher abundance. Alpha diversity analysis showed a low diversity (Shannon and Simpson index); Chao observed species in both Regions. These results suggest that the bacteria identified in these genera may possess the ability to degrade petroleum, and further studies in the future should elucidate their role in petroleum degradation.https://www.mdpi.com/2076-3417/13/12/6922bacterialsoil contaminatedpetroleummicrobial isolation |
| spellingShingle | Raul García-García Virgilio Bocanegra-García Lourdes Vital-López Jaime García-Mena Marco Antonio Zamora-Antuñano María Antonia Cruz-Hernández Juvenal Rodríguez-Reséndiz Alberto Mendoza-Herrera Assessment of the Microbial Communities in Soil Contaminated with Petroleum Using Next-Generation Sequencing Tools Applied Sciences bacterial soil contaminated petroleum microbial isolation |
| title | Assessment of the Microbial Communities in Soil Contaminated with Petroleum Using Next-Generation Sequencing Tools |
| title_full | Assessment of the Microbial Communities in Soil Contaminated with Petroleum Using Next-Generation Sequencing Tools |
| title_fullStr | Assessment of the Microbial Communities in Soil Contaminated with Petroleum Using Next-Generation Sequencing Tools |
| title_full_unstemmed | Assessment of the Microbial Communities in Soil Contaminated with Petroleum Using Next-Generation Sequencing Tools |
| title_short | Assessment of the Microbial Communities in Soil Contaminated with Petroleum Using Next-Generation Sequencing Tools |
| title_sort | assessment of the microbial communities in soil contaminated with petroleum using next generation sequencing tools |
| topic | bacterial soil contaminated petroleum microbial isolation |
| url | https://www.mdpi.com/2076-3417/13/12/6922 |
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