Phototoxicity of Pheophorbide A Extracted from Gracilaria Lemaneiformis on Six Kinds of Cancer Cells
Objective To investigate the photodynamic antitumor activity and chemical characteristics of pheophorbide A (PPBa)in vitro. Methods Breast cancer cells (MCF-7), lung cancer cells (A549), cervical cancer cells (HeLa), and three kinds of hepatoma cells (HepG2, hep3B and sk-Hep1) were planted in 96-wel...
| Main Authors: | , , , , , |
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| Format: | Article |
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Magazine House of Cancer Research on Prevention and Treatment
2022-08-01
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| Series: | Zhongliu Fangzhi Yanjiu |
| Subjects: | |
| Online Access: | http://www.zlfzyj.com/EN/10.3971/j.issn.1000-8578.2022.22.0172 |
| _version_ | 1798036578338078720 |
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| author | HUANG Xixiang CAI Xiaoxuan WANG Tianji HUANG Miaoen LI Li LYU Yingnian |
| author_facet | HUANG Xixiang CAI Xiaoxuan WANG Tianji HUANG Miaoen LI Li LYU Yingnian |
| author_sort | HUANG Xixiang |
| collection | DOAJ |
| description | Objective To investigate the photodynamic antitumor activity and chemical characteristics of pheophorbide A (PPBa)in vitro. Methods Breast cancer cells (MCF-7), lung cancer cells (A549), cervical cancer cells (HeLa), and three kinds of hepatoma cells (HepG2, hep3B and sk-Hep1) were planted in 96-well plates.The effects of light and dark toxicity, light intensity, and drug concentration on the phototoxicity of PPBa were investigated by MTT, and the uptake of PPBa was observed by Hoechst staining under a confocal microscope.The production of singlet oxygen was observed by flow cytometry and confocal microscopy with the reactive oxygen species detection kit.The photobleaching of PPBa was investigated by measuring the absorbance by a microplate reader according to the luminescence characteristics of PPBa. Results PPBa showed strong phototoxicity and low dark toxicity to six kinds of cancer cells, and IC50 values to cancer cells were as follows: MCF-7:1.033 μmol/L, A549:1.911 μmol/L, HeLa: 2.319 μmol/L, HepG2:2.015 μmol/L, Hep3B: 2.089 μmol/L, sk-Hep1:2.467 μmol/L.The main uptake site of PPBa was the cytoplasm.The production of singlet oxygen was strongly dependent on the administration concentration, and photobleaching was very low. Conclusion For the six kinds of cancer cells, PPBa has the highest phototoxicity to breast cancer cells (MCF-7), with excellent properties and ideal photosensitizer characteristics. |
| first_indexed | 2024-04-11T21:14:56Z |
| format | Article |
| id | doaj.art-8cbff27319764d2bb14d43bbfd69a11b |
| institution | Directory Open Access Journal |
| issn | 1000-8578 |
| language | zho |
| last_indexed | 2024-04-11T21:14:56Z |
| publishDate | 2022-08-01 |
| publisher | Magazine House of Cancer Research on Prevention and Treatment |
| record_format | Article |
| series | Zhongliu Fangzhi Yanjiu |
| spelling | doaj.art-8cbff27319764d2bb14d43bbfd69a11b2022-12-22T04:02:50ZzhoMagazine House of Cancer Research on Prevention and TreatmentZhongliu Fangzhi Yanjiu1000-85782022-08-0149878078510.3971/j.issn.1000-8578.2022.22.01728578.2022.22.0172Phototoxicity of Pheophorbide A Extracted from Gracilaria Lemaneiformis on Six Kinds of Cancer CellsHUANG Xixiang0CAI Xiaoxuan1WANG Tianji2HUANG Miaoen3LI Li4LYU Yingnian5School of Pharmacy, Guangdong Medical University, Zhanjiang 524023, ChinaSchool of Pharmacy, Guangdong Medical University, Zhanjiang 524023, ChinaSchool of Pharmacy, Guangdong Medical University, Zhanjiang 524023, ChinaSchool of Pharmacy, Guangdong Medical University, Zhanjiang 524023, ChinaSchool of Pharmacy, Guangdong Medical University, Zhanjiang 524023, ChinaSchool of Pharmacy, Guangdong Medical University, Zhanjiang 524023, ChinaObjective To investigate the photodynamic antitumor activity and chemical characteristics of pheophorbide A (PPBa)in vitro. Methods Breast cancer cells (MCF-7), lung cancer cells (A549), cervical cancer cells (HeLa), and three kinds of hepatoma cells (HepG2, hep3B and sk-Hep1) were planted in 96-well plates.The effects of light and dark toxicity, light intensity, and drug concentration on the phototoxicity of PPBa were investigated by MTT, and the uptake of PPBa was observed by Hoechst staining under a confocal microscope.The production of singlet oxygen was observed by flow cytometry and confocal microscopy with the reactive oxygen species detection kit.The photobleaching of PPBa was investigated by measuring the absorbance by a microplate reader according to the luminescence characteristics of PPBa. Results PPBa showed strong phototoxicity and low dark toxicity to six kinds of cancer cells, and IC50 values to cancer cells were as follows: MCF-7:1.033 μmol/L, A549:1.911 μmol/L, HeLa: 2.319 μmol/L, HepG2:2.015 μmol/L, Hep3B: 2.089 μmol/L, sk-Hep1:2.467 μmol/L.The main uptake site of PPBa was the cytoplasm.The production of singlet oxygen was strongly dependent on the administration concentration, and photobleaching was very low. Conclusion For the six kinds of cancer cells, PPBa has the highest phototoxicity to breast cancer cells (MCF-7), with excellent properties and ideal photosensitizer characteristics.http://www.zlfzyj.com/EN/10.3971/j.issn.1000-8578.2022.22.0172pheophorbide aphotodynamic therapyanti-tumor effectphototoxicity |
| spellingShingle | HUANG Xixiang CAI Xiaoxuan WANG Tianji HUANG Miaoen LI Li LYU Yingnian Phototoxicity of Pheophorbide A Extracted from Gracilaria Lemaneiformis on Six Kinds of Cancer Cells Zhongliu Fangzhi Yanjiu pheophorbide a photodynamic therapy anti-tumor effect phototoxicity |
| title | Phototoxicity of Pheophorbide A Extracted from Gracilaria Lemaneiformis on Six Kinds of Cancer Cells |
| title_full | Phototoxicity of Pheophorbide A Extracted from Gracilaria Lemaneiformis on Six Kinds of Cancer Cells |
| title_fullStr | Phototoxicity of Pheophorbide A Extracted from Gracilaria Lemaneiformis on Six Kinds of Cancer Cells |
| title_full_unstemmed | Phototoxicity of Pheophorbide A Extracted from Gracilaria Lemaneiformis on Six Kinds of Cancer Cells |
| title_short | Phototoxicity of Pheophorbide A Extracted from Gracilaria Lemaneiformis on Six Kinds of Cancer Cells |
| title_sort | phototoxicity of pheophorbide a extracted from gracilaria lemaneiformis on six kinds of cancer cells |
| topic | pheophorbide a photodynamic therapy anti-tumor effect phototoxicity |
| url | http://www.zlfzyj.com/EN/10.3971/j.issn.1000-8578.2022.22.0172 |
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