Adhesion GPCRs are widely expressed throughout the subsections of the gastrointestinal tract
<p>Abstract</p> <p>Background</p> <p>G protein-coupled receptors (GPCRs) represent one of the largest families of transmembrane receptors and the most common drug target. The <it>Adhesion</it> subfamily is the second largest one of GPCRs and its several memb...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2012-09-01
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| Series: | BMC Gastroenterology |
| Online Access: | http://www.biomedcentral.com/1471-230X/12/134 |
| _version_ | 1811244761242140672 |
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| author | Badiali Luca Cedernaes Jonathan Olszewski Pawel K Nylander Olof Vergoni Anna V Schiöth Helgi B |
| author_facet | Badiali Luca Cedernaes Jonathan Olszewski Pawel K Nylander Olof Vergoni Anna V Schiöth Helgi B |
| author_sort | Badiali Luca |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>G protein-coupled receptors (GPCRs) represent one of the largest families of transmembrane receptors and the most common drug target. The <it>Adhesion</it> subfamily is the second largest one of GPCRs and its several members are known to mediate neural development and immune system functioning through cell-cell and cell-matrix interactions. The distribution of these receptors has not been characterized in detail in the gastrointestinal (GI) tract. Here we present the first comprehensive anatomical profiling of mRNA expression of all 30 <it>Adhesion</it> GPCRs in the rat GI tract divided into twelve subsegments.</p> <p>Methods</p> <p>Using RT-qPCR, we studied the expression of <it>Adhesion</it> GPCRs in the esophagus, the corpus and antrum of the stomach, the proximal and distal parts of the duodenum, ileum, jejunum and colon, and the cecum.</p> <p>Results</p> <p>We found that twenty-one <it>Adhesion</it> GPCRs (70%) had a widespread (expressed in five or more segments) or ubiquitous (expressed in eleven or more segments) distribution, seven (23%) were restricted to a few segments of the GI tract and two were not expressed in any segment. Most notably, almost all Group III members were ubiquitously expressed, while the restricted expression was characteristic for the majority of group VII members, hinting at more specific/localized roles for some of these receptors.</p> <p>Conclusions</p> <p>Overall, the distribution of <it>Adhesion</it> GPCRs points to their important role in GI tract functioning and defines them as a potentially crucial target for pharmacological interventions.</p> |
| first_indexed | 2024-04-12T14:30:35Z |
| format | Article |
| id | doaj.art-8cc1f4d79cc94b8da36c1ef33c9b4e1a |
| institution | Directory Open Access Journal |
| issn | 1471-230X |
| language | English |
| last_indexed | 2024-04-12T14:30:35Z |
| publishDate | 2012-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Gastroenterology |
| spelling | doaj.art-8cc1f4d79cc94b8da36c1ef33c9b4e1a2022-12-22T03:29:18ZengBMCBMC Gastroenterology1471-230X2012-09-0112113410.1186/1471-230X-12-134Adhesion GPCRs are widely expressed throughout the subsections of the gastrointestinal tractBadiali LucaCedernaes JonathanOlszewski Pawel KNylander OlofVergoni Anna VSchiöth Helgi B<p>Abstract</p> <p>Background</p> <p>G protein-coupled receptors (GPCRs) represent one of the largest families of transmembrane receptors and the most common drug target. The <it>Adhesion</it> subfamily is the second largest one of GPCRs and its several members are known to mediate neural development and immune system functioning through cell-cell and cell-matrix interactions. The distribution of these receptors has not been characterized in detail in the gastrointestinal (GI) tract. Here we present the first comprehensive anatomical profiling of mRNA expression of all 30 <it>Adhesion</it> GPCRs in the rat GI tract divided into twelve subsegments.</p> <p>Methods</p> <p>Using RT-qPCR, we studied the expression of <it>Adhesion</it> GPCRs in the esophagus, the corpus and antrum of the stomach, the proximal and distal parts of the duodenum, ileum, jejunum and colon, and the cecum.</p> <p>Results</p> <p>We found that twenty-one <it>Adhesion</it> GPCRs (70%) had a widespread (expressed in five or more segments) or ubiquitous (expressed in eleven or more segments) distribution, seven (23%) were restricted to a few segments of the GI tract and two were not expressed in any segment. Most notably, almost all Group III members were ubiquitously expressed, while the restricted expression was characteristic for the majority of group VII members, hinting at more specific/localized roles for some of these receptors.</p> <p>Conclusions</p> <p>Overall, the distribution of <it>Adhesion</it> GPCRs points to their important role in GI tract functioning and defines them as a potentially crucial target for pharmacological interventions.</p>http://www.biomedcentral.com/1471-230X/12/134 |
| spellingShingle | Badiali Luca Cedernaes Jonathan Olszewski Pawel K Nylander Olof Vergoni Anna V Schiöth Helgi B Adhesion GPCRs are widely expressed throughout the subsections of the gastrointestinal tract BMC Gastroenterology |
| title | Adhesion GPCRs are widely expressed throughout the subsections of the gastrointestinal tract |
| title_full | Adhesion GPCRs are widely expressed throughout the subsections of the gastrointestinal tract |
| title_fullStr | Adhesion GPCRs are widely expressed throughout the subsections of the gastrointestinal tract |
| title_full_unstemmed | Adhesion GPCRs are widely expressed throughout the subsections of the gastrointestinal tract |
| title_short | Adhesion GPCRs are widely expressed throughout the subsections of the gastrointestinal tract |
| title_sort | adhesion gpcrs are widely expressed throughout the subsections of the gastrointestinal tract |
| url | http://www.biomedcentral.com/1471-230X/12/134 |
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