Nondestructive protein sampling with electroporation facilitates profiling of spatial differential protein expression in breast tumors in vivo
Abstract Excision tissue biopsy, while central to cancer treatment and precision medicine, presents risks to the patient and does not provide a sufficiently broad and faithful representation of the heterogeneity of solid tumors. Here we introduce e-biopsy—a novel concept for molecular profiling of s...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-09-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-19984-x |
| _version_ | 1811208811838439424 |
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| author | Edward Vitkin Amrita Singh Julia Wise Shay Ben-Elazar Zohar Yakhini Alexander Golberg |
| author_facet | Edward Vitkin Amrita Singh Julia Wise Shay Ben-Elazar Zohar Yakhini Alexander Golberg |
| author_sort | Edward Vitkin |
| collection | DOAJ |
| description | Abstract Excision tissue biopsy, while central to cancer treatment and precision medicine, presents risks to the patient and does not provide a sufficiently broad and faithful representation of the heterogeneity of solid tumors. Here we introduce e-biopsy—a novel concept for molecular profiling of solid tumors using molecular sampling with electroporation. As e-biopsy provides access to the molecular composition of a solid tumor by permeabilization of the cell membrane, it facilitates tumor diagnostics without tissue resection. Furthermore, thanks to its non tissue destructive characteristics, e-biopsy enables probing the solid tumor multiple times in several distinct locations in the same procedure, thereby enabling the spatial profiling of tumor molecular heterogeneity.We demonstrate e-biopsy in vivo, using the 4T1 breast cancer model in mice to assess its performance, as well as the inferred spatial differential protein expression. In particular, we show that proteomic profiles obtained via e-biopsy in vivo distinguish the tumors from healthy breast tissue and reflect spatial tumor differential protein expression. E-biopsy provides a completely new molecular sampling modality for solid tumors molecular cartography, providing information that potentially enables more rapid and sensitive detection at lesser risk, as well as more precise personalized medicine. |
| first_indexed | 2024-04-12T04:29:15Z |
| format | Article |
| id | doaj.art-8cc44bf558434fbd84f52a513ef83fd2 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-12T04:29:15Z |
| publishDate | 2022-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-8cc44bf558434fbd84f52a513ef83fd22022-12-22T03:48:00ZengNature PortfolioScientific Reports2045-23222022-09-0112111210.1038/s41598-022-19984-xNondestructive protein sampling with electroporation facilitates profiling of spatial differential protein expression in breast tumors in vivoEdward Vitkin0Amrita Singh1Julia Wise2Shay Ben-Elazar3Zohar Yakhini4Alexander Golberg5School of Computer Science, Reichman University (IDC Herzliya)Porter School of Environment and Earth Sciences, Tel Aviv UniversityPorter School of Environment and Earth Sciences, Tel Aviv UniversitySchool of Computer Science, Reichman University (IDC Herzliya)School of Computer Science, Reichman University (IDC Herzliya)Porter School of Environment and Earth Sciences, Tel Aviv UniversityAbstract Excision tissue biopsy, while central to cancer treatment and precision medicine, presents risks to the patient and does not provide a sufficiently broad and faithful representation of the heterogeneity of solid tumors. Here we introduce e-biopsy—a novel concept for molecular profiling of solid tumors using molecular sampling with electroporation. As e-biopsy provides access to the molecular composition of a solid tumor by permeabilization of the cell membrane, it facilitates tumor diagnostics without tissue resection. Furthermore, thanks to its non tissue destructive characteristics, e-biopsy enables probing the solid tumor multiple times in several distinct locations in the same procedure, thereby enabling the spatial profiling of tumor molecular heterogeneity.We demonstrate e-biopsy in vivo, using the 4T1 breast cancer model in mice to assess its performance, as well as the inferred spatial differential protein expression. In particular, we show that proteomic profiles obtained via e-biopsy in vivo distinguish the tumors from healthy breast tissue and reflect spatial tumor differential protein expression. E-biopsy provides a completely new molecular sampling modality for solid tumors molecular cartography, providing information that potentially enables more rapid and sensitive detection at lesser risk, as well as more precise personalized medicine.https://doi.org/10.1038/s41598-022-19984-x |
| spellingShingle | Edward Vitkin Amrita Singh Julia Wise Shay Ben-Elazar Zohar Yakhini Alexander Golberg Nondestructive protein sampling with electroporation facilitates profiling of spatial differential protein expression in breast tumors in vivo Scientific Reports |
| title | Nondestructive protein sampling with electroporation facilitates profiling of spatial differential protein expression in breast tumors in vivo |
| title_full | Nondestructive protein sampling with electroporation facilitates profiling of spatial differential protein expression in breast tumors in vivo |
| title_fullStr | Nondestructive protein sampling with electroporation facilitates profiling of spatial differential protein expression in breast tumors in vivo |
| title_full_unstemmed | Nondestructive protein sampling with electroporation facilitates profiling of spatial differential protein expression in breast tumors in vivo |
| title_short | Nondestructive protein sampling with electroporation facilitates profiling of spatial differential protein expression in breast tumors in vivo |
| title_sort | nondestructive protein sampling with electroporation facilitates profiling of spatial differential protein expression in breast tumors in vivo |
| url | https://doi.org/10.1038/s41598-022-19984-x |
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