Quinoa Ameliorates Hepatic Steatosis, Oxidative Stress, Inflammation and Regulates the Gut Microbiota in Nonalcoholic Fatty Liver Disease Rats
A long-term high-fat diet causes hepatic steatosis, which further leads to oxidative stress and inflammation. In this study, we firstly investigated the regulation effects of different amounts of quinoa on hepatic steatosis, oxidative stress, and inflammation of rats fed a high-fat diet, then the gu...
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MDPI AG
2023-04-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/9/1780 |
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| author | Lingyue Zhong Wei Lyu Zihan Lin Jun Lu Yanlou Geng Lihua Song Heng Zhang |
| author_facet | Lingyue Zhong Wei Lyu Zihan Lin Jun Lu Yanlou Geng Lihua Song Heng Zhang |
| author_sort | Lingyue Zhong |
| collection | DOAJ |
| description | A long-term high-fat diet causes hepatic steatosis, which further leads to oxidative stress and inflammation. In this study, we firstly investigated the regulation effects of different amounts of quinoa on hepatic steatosis, oxidative stress, and inflammation of rats fed a high-fat diet, then the gut microbiota was dynamically determined. Sprague–Dawley (SD, male) rats were randomized into four groups: normal controls (NC, fed standard chow), model groups (HF, fed a high-fat diet), low quinoa intake (HF + LQ), and high quinoa intake (HF + HQ) groups, which were supplemented with 9% and 27% quinoa in the high-fat feed (equivalent to 100 g/day and 300 g/day human intake, respectively). The results showed that quinoa intake significantly inhibited the hepatomegaly and splenomegaly, ameliorated hepatic steatosis pathologically; effectively rescued the decrease in the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) and the increase in malondialdehyde (MDA). The levels of tumor necrosis factor-α (TNF-α), interleukin-10 (IL-10), transforming growth factor-β (TGF-β), and leptin in rats of two quinoa groups were close to those of the NC group. Besides, high quinoa intake significantly increased the relative abundance of <i>Akkermansia</i>, and low quinoa intake significantly increased the relative abundance of <i>Blautia</i> at the genus level. The relative abundances of <i>Blautia</i> and <i>Dorea</i> in rats in the HF + HQ group were lower than those in rats in the HF + LQ group. In addition, the relative abundances of <i>Clostridium</i> and <i>Turicibacter</i> of rats in the two quinoa intervention groups were lower than those of rats in the HF group after 12 weeks of intervention. In summary, quinoa exhibits a series of beneficial effects in the prevention of nonalcoholic fatty liver disease (NAFLD) and is suggested to be a component of a daily diet for the prevention of NAFLD. |
| first_indexed | 2024-03-11T04:19:21Z |
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| language | English |
| last_indexed | 2024-03-11T04:19:21Z |
| publishDate | 2023-04-01 |
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| spelling | doaj.art-8cc736cad84745db8dfed84e47b46bf72023-11-17T22:54:46ZengMDPI AGFoods2304-81582023-04-01129178010.3390/foods12091780Quinoa Ameliorates Hepatic Steatosis, Oxidative Stress, Inflammation and Regulates the Gut Microbiota in Nonalcoholic Fatty Liver Disease RatsLingyue Zhong0Wei Lyu1Zihan Lin2Jun Lu3Yanlou Geng4Lihua Song5Heng Zhang6School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaNational Semi-Arid Agriculture Engineering Technology Research Center, Shijiazhuang 050051, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaCAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 201602, ChinaNational Semi-Arid Agriculture Engineering Technology Research Center, Shijiazhuang 050051, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaCAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 201602, ChinaA long-term high-fat diet causes hepatic steatosis, which further leads to oxidative stress and inflammation. In this study, we firstly investigated the regulation effects of different amounts of quinoa on hepatic steatosis, oxidative stress, and inflammation of rats fed a high-fat diet, then the gut microbiota was dynamically determined. Sprague–Dawley (SD, male) rats were randomized into four groups: normal controls (NC, fed standard chow), model groups (HF, fed a high-fat diet), low quinoa intake (HF + LQ), and high quinoa intake (HF + HQ) groups, which were supplemented with 9% and 27% quinoa in the high-fat feed (equivalent to 100 g/day and 300 g/day human intake, respectively). The results showed that quinoa intake significantly inhibited the hepatomegaly and splenomegaly, ameliorated hepatic steatosis pathologically; effectively rescued the decrease in the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) and the increase in malondialdehyde (MDA). The levels of tumor necrosis factor-α (TNF-α), interleukin-10 (IL-10), transforming growth factor-β (TGF-β), and leptin in rats of two quinoa groups were close to those of the NC group. Besides, high quinoa intake significantly increased the relative abundance of <i>Akkermansia</i>, and low quinoa intake significantly increased the relative abundance of <i>Blautia</i> at the genus level. The relative abundances of <i>Blautia</i> and <i>Dorea</i> in rats in the HF + HQ group were lower than those in rats in the HF + LQ group. In addition, the relative abundances of <i>Clostridium</i> and <i>Turicibacter</i> of rats in the two quinoa intervention groups were lower than those of rats in the HF group after 12 weeks of intervention. In summary, quinoa exhibits a series of beneficial effects in the prevention of nonalcoholic fatty liver disease (NAFLD) and is suggested to be a component of a daily diet for the prevention of NAFLD.https://www.mdpi.com/2304-8158/12/9/1780gut microbiotahigh fat dietNAFLDquinoa intake |
| spellingShingle | Lingyue Zhong Wei Lyu Zihan Lin Jun Lu Yanlou Geng Lihua Song Heng Zhang Quinoa Ameliorates Hepatic Steatosis, Oxidative Stress, Inflammation and Regulates the Gut Microbiota in Nonalcoholic Fatty Liver Disease Rats Foods gut microbiota high fat diet NAFLD quinoa intake |
| title | Quinoa Ameliorates Hepatic Steatosis, Oxidative Stress, Inflammation and Regulates the Gut Microbiota in Nonalcoholic Fatty Liver Disease Rats |
| title_full | Quinoa Ameliorates Hepatic Steatosis, Oxidative Stress, Inflammation and Regulates the Gut Microbiota in Nonalcoholic Fatty Liver Disease Rats |
| title_fullStr | Quinoa Ameliorates Hepatic Steatosis, Oxidative Stress, Inflammation and Regulates the Gut Microbiota in Nonalcoholic Fatty Liver Disease Rats |
| title_full_unstemmed | Quinoa Ameliorates Hepatic Steatosis, Oxidative Stress, Inflammation and Regulates the Gut Microbiota in Nonalcoholic Fatty Liver Disease Rats |
| title_short | Quinoa Ameliorates Hepatic Steatosis, Oxidative Stress, Inflammation and Regulates the Gut Microbiota in Nonalcoholic Fatty Liver Disease Rats |
| title_sort | quinoa ameliorates hepatic steatosis oxidative stress inflammation and regulates the gut microbiota in nonalcoholic fatty liver disease rats |
| topic | gut microbiota high fat diet NAFLD quinoa intake |
| url | https://www.mdpi.com/2304-8158/12/9/1780 |
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