Anti-Inflammatory and Antiatopic Effects of <i>Rorippa cantoniensis</i> (Lour.) Ohwi in RAW 264.7 and HaCaT Cells
This study evaluated the effects of <i>Rorippa cantoniensis</i> (Lour.) ohwi extract (RCE) on factors associated with inflammation-related skin lesions in RAW 264.7 and HaCaT cells. RCE inhibited the levels of proinflammatory mediators and cytokines such as nitric oxide (NO), prostagland...
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MDPI AG
2023-07-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/14/5463 |
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| author | Min-Jin Kim Buyng Su Hwang Yong Hwang Yong Tae Jeong Dae Won Jeong Young Taek Oh |
| author_facet | Min-Jin Kim Buyng Su Hwang Yong Hwang Yong Tae Jeong Dae Won Jeong Young Taek Oh |
| author_sort | Min-Jin Kim |
| collection | DOAJ |
| description | This study evaluated the effects of <i>Rorippa cantoniensis</i> (Lour.) ohwi extract (RCE) on factors associated with inflammation-related skin lesions in RAW 264.7 and HaCaT cells. RCE inhibited the levels of proinflammatory mediators and cytokines such as nitric oxide (NO), prostaglandin E2 (PGE<sub>2</sub>), interleukin (IL)-6, and tumor necrosis factor (TNF)-α in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. In addition, RCE significantly inhibited the expression of chemokines and cytokines such as MDC/CCL22, TARC/CCL17, RANTES/CCL5, CTSS, IL-6, IL-1β, and TNF-α in HaCaT cells costimulated by TNF-α and interferon (IFN)-γ in a concentration-dependent manner. These results suggest that RCE attenuated the TNF-α- and IFN-γ-induced release of proinflammatory chemokines and cytokines probably by suppressing the activation of MAPK (JNK and p38), NF-κB, and STAT1 signaling. Moreover, RCE significantly increased the expression of skin components such as hyaluronic acid and aquaporin, which play important roles in the physical and chemical barriers of the skin. These results suggest that RCE has significant anti-inflammatory and antiatopic activities, which may be beneficial for the topical treatment of inflammatory skin disorders. |
| first_indexed | 2024-03-11T00:47:04Z |
| format | Article |
| id | doaj.art-8cc7ac1ea4e04750942130ca3f06fe60 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T00:47:04Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-8cc7ac1ea4e04750942130ca3f06fe602023-11-18T20:42:29ZengMDPI AGMolecules1420-30492023-07-012814546310.3390/molecules28145463Anti-Inflammatory and Antiatopic Effects of <i>Rorippa cantoniensis</i> (Lour.) Ohwi in RAW 264.7 and HaCaT CellsMin-Jin Kim0Buyng Su Hwang1Yong Hwang2Yong Tae Jeong3Dae Won Jeong4Young Taek Oh5Nakdonggang National Institute of Biological Resources, 137, Donam 2-gil, Sangju-si 37242, Republic of KoreaNakdonggang National Institute of Biological Resources, 137, Donam 2-gil, Sangju-si 37242, Republic of KoreaNakdonggang National Institute of Biological Resources, 137, Donam 2-gil, Sangju-si 37242, Republic of KoreaNakdonggang National Institute of Biological Resources, 137, Donam 2-gil, Sangju-si 37242, Republic of KoreaNakdonggang National Institute of Biological Resources, 137, Donam 2-gil, Sangju-si 37242, Republic of KoreaNakdonggang National Institute of Biological Resources, 137, Donam 2-gil, Sangju-si 37242, Republic of KoreaThis study evaluated the effects of <i>Rorippa cantoniensis</i> (Lour.) ohwi extract (RCE) on factors associated with inflammation-related skin lesions in RAW 264.7 and HaCaT cells. RCE inhibited the levels of proinflammatory mediators and cytokines such as nitric oxide (NO), prostaglandin E2 (PGE<sub>2</sub>), interleukin (IL)-6, and tumor necrosis factor (TNF)-α in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. In addition, RCE significantly inhibited the expression of chemokines and cytokines such as MDC/CCL22, TARC/CCL17, RANTES/CCL5, CTSS, IL-6, IL-1β, and TNF-α in HaCaT cells costimulated by TNF-α and interferon (IFN)-γ in a concentration-dependent manner. These results suggest that RCE attenuated the TNF-α- and IFN-γ-induced release of proinflammatory chemokines and cytokines probably by suppressing the activation of MAPK (JNK and p38), NF-κB, and STAT1 signaling. Moreover, RCE significantly increased the expression of skin components such as hyaluronic acid and aquaporin, which play important roles in the physical and chemical barriers of the skin. These results suggest that RCE has significant anti-inflammatory and antiatopic activities, which may be beneficial for the topical treatment of inflammatory skin disorders.https://www.mdpi.com/1420-3049/28/14/5463inflammatoryantiatopic activityMAPKNF-κBSTAT1<i>Rorippa cantoniensis</i> (Lour.) Ohwi |
| spellingShingle | Min-Jin Kim Buyng Su Hwang Yong Hwang Yong Tae Jeong Dae Won Jeong Young Taek Oh Anti-Inflammatory and Antiatopic Effects of <i>Rorippa cantoniensis</i> (Lour.) Ohwi in RAW 264.7 and HaCaT Cells Molecules inflammatory antiatopic activity MAPK NF-κB STAT1 <i>Rorippa cantoniensis</i> (Lour.) Ohwi |
| title | Anti-Inflammatory and Antiatopic Effects of <i>Rorippa cantoniensis</i> (Lour.) Ohwi in RAW 264.7 and HaCaT Cells |
| title_full | Anti-Inflammatory and Antiatopic Effects of <i>Rorippa cantoniensis</i> (Lour.) Ohwi in RAW 264.7 and HaCaT Cells |
| title_fullStr | Anti-Inflammatory and Antiatopic Effects of <i>Rorippa cantoniensis</i> (Lour.) Ohwi in RAW 264.7 and HaCaT Cells |
| title_full_unstemmed | Anti-Inflammatory and Antiatopic Effects of <i>Rorippa cantoniensis</i> (Lour.) Ohwi in RAW 264.7 and HaCaT Cells |
| title_short | Anti-Inflammatory and Antiatopic Effects of <i>Rorippa cantoniensis</i> (Lour.) Ohwi in RAW 264.7 and HaCaT Cells |
| title_sort | anti inflammatory and antiatopic effects of i rorippa cantoniensis i lour ohwi in raw 264 7 and hacat cells |
| topic | inflammatory antiatopic activity MAPK NF-κB STAT1 <i>Rorippa cantoniensis</i> (Lour.) Ohwi |
| url | https://www.mdpi.com/1420-3049/28/14/5463 |
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