Green seaweed Caulerpa racemosa - Chemical constituents, cytotoxicity in breast cancer cells and molecular docking simulation
Marine and terrestrial organisms are rich in chemical compounds with medicinal and pharmacological properties, including antitumor agents for chemoprevention. Caulerpa racemosa, a marine species, is a potential source of novel compounds with therapeutic agents for human cancer. This study aimed to d...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-06-01
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| Series: | Journal of Agriculture and Food Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S266615432300128X |
| _version_ | 1797823094744678400 |
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| author | Grace Sanger Djuhria Wonggo Nurmelita Taher Verly Dotulong Aurielle Annalicia Setiawan Happy Kurnia Permatasari Sidik Maulana Fahrul Nurkolis Apollinaire Tsopmo Bonglee Kim |
| author_facet | Grace Sanger Djuhria Wonggo Nurmelita Taher Verly Dotulong Aurielle Annalicia Setiawan Happy Kurnia Permatasari Sidik Maulana Fahrul Nurkolis Apollinaire Tsopmo Bonglee Kim |
| author_sort | Grace Sanger |
| collection | DOAJ |
| description | Marine and terrestrial organisms are rich in chemical compounds with medicinal and pharmacological properties, including antitumor agents for chemoprevention. Caulerpa racemosa, a marine species, is a potential source of novel compounds with therapeutic agents for human cancer. This study aimed to determine the anticancer activity of C. racemosa extracts in breast cancer cells, identify compounds, and determine the mechanism using computational models. Seaweed (C. racemosa) was taken from North Sulawesi, Indonesia; Followed by authentication and identification according to the previously published protocol and extracted with three different solvent: hexane, ethyl acetate, and ethanol. C. racemosa were evaluated for cytotoxicity against breast cancer MCF-7 cells using MTT (3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide) assay. Antioxidant activities were assessed based on free radical scavenging (2,2-diphenyl-1-picrylhydrazyl (DPPH)) and ferric-reducing antioxidant power (FRAP) assays. The phytochemical constituents were identified with a liquid chromatography-electrospray ionization quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF-MS) system. The interaction of the identified compounds with human epidermal growth factor 2 (HER2) protein was achieved by molecular docking with PyRx-vina application and protein-ligand complex visualization. The hexane extract exhibited the highest cytotoxicity against breast cancer cells, followed by the ethyl acetate and the ethanol extract (IC50 23.7 ± 2.0; 66.7 ± 5.8 and 182.7 ± 14.3 μg/mL). The best antioxidant sample for DPPH was the ethyl acetate extract (IC50 21.5 ± 2.0 μg/mL) while the hexane extract was the most active in the FRAP value (14.5 ± 1.3 μg gallic acid equivalent/g). Data from LC-ESI-QTOF-MS allowed the identification of 21 compounds. The molecular docking study showed that 12 of the compounds could prevent tumors in breast cancer by acting as inhibitors of the anti-apoptotic HER2 protein. C. racemosa has potential in the chemoprevention of breast cancer through its radical scavenging capacity and inhibition of the HER2 protein. More studies are needed to investigate the efficacy of extracts in different models. |
| first_indexed | 2024-03-13T10:18:56Z |
| format | Article |
| id | doaj.art-8cc85dbb3f9d425395a98027d03f2904 |
| institution | Directory Open Access Journal |
| issn | 2666-1543 |
| language | English |
| last_indexed | 2024-03-13T10:18:56Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Agriculture and Food Research |
| spelling | doaj.art-8cc85dbb3f9d425395a98027d03f29042023-05-21T04:35:54ZengElsevierJournal of Agriculture and Food Research2666-15432023-06-0112100621Green seaweed Caulerpa racemosa - Chemical constituents, cytotoxicity in breast cancer cells and molecular docking simulationGrace Sanger0Djuhria Wonggo1Nurmelita Taher2Verly Dotulong3Aurielle Annalicia Setiawan4Happy Kurnia Permatasari5Sidik Maulana6Fahrul Nurkolis7Apollinaire Tsopmo8Bonglee Kim9Department of Fishery Product Technology, Faculty of Fishery and Marine Science, Sam Ratulangi University, Manado, 95115, Indonesia; Corresponding author.Department of Fishery Product Technology, Faculty of Fishery and Marine Science, Sam Ratulangi University, Manado, 95115, IndonesiaDepartment of Fishery Product Technology, Faculty of Fishery and Marine Science, Sam Ratulangi University, Manado, 95115, IndonesiaDepartment of Fishery Product Technology, Faculty of Fishery and Marine Science, Sam Ratulangi University, Manado, 95115, IndonesiaMedical Study Programme, Faculty of Medicine, University of Brawijaya, IndonesiaDepartment of Biochemistry and Biomolecular, Faculty of Medicine, University of Brawijaya, IndonesiaDepartment of Medical-Surgical Nursing, Faculty of Nursing, Universitas Padjadjaran, Sumedang, 45363, IndonesiaDepartment of Biological Sciences, Faculty of Sciences and Technology, State Islamic University of Sunan Kalijaga (UIN Sunan Kalijaga), Yogyakarta, 55281, IndonesiaFood Science and Nutrition Program, Department of Chemistry, Carleton University, 1125 Colonel by Drive, Ottawa, ON, K1S 5B6, Canada; Institute of Biochemistry, Carleton University, Ottawa, ON, K1S 5B6, CanadaDepartment of Pathology, College of Korean Medicine, Kyung Hee University, Hoegidong Dongdaemun-gu, Seoul, 02447, Republic of Korea; Korean Medicine-Based Drug Repositioning Cancer Research Center, College of Korean Medicine, Kyung Hee University, Seoul, 02447, Republic of KoreaMarine and terrestrial organisms are rich in chemical compounds with medicinal and pharmacological properties, including antitumor agents for chemoprevention. Caulerpa racemosa, a marine species, is a potential source of novel compounds with therapeutic agents for human cancer. This study aimed to determine the anticancer activity of C. racemosa extracts in breast cancer cells, identify compounds, and determine the mechanism using computational models. Seaweed (C. racemosa) was taken from North Sulawesi, Indonesia; Followed by authentication and identification according to the previously published protocol and extracted with three different solvent: hexane, ethyl acetate, and ethanol. C. racemosa were evaluated for cytotoxicity against breast cancer MCF-7 cells using MTT (3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide) assay. Antioxidant activities were assessed based on free radical scavenging (2,2-diphenyl-1-picrylhydrazyl (DPPH)) and ferric-reducing antioxidant power (FRAP) assays. The phytochemical constituents were identified with a liquid chromatography-electrospray ionization quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF-MS) system. The interaction of the identified compounds with human epidermal growth factor 2 (HER2) protein was achieved by molecular docking with PyRx-vina application and protein-ligand complex visualization. The hexane extract exhibited the highest cytotoxicity against breast cancer cells, followed by the ethyl acetate and the ethanol extract (IC50 23.7 ± 2.0; 66.7 ± 5.8 and 182.7 ± 14.3 μg/mL). The best antioxidant sample for DPPH was the ethyl acetate extract (IC50 21.5 ± 2.0 μg/mL) while the hexane extract was the most active in the FRAP value (14.5 ± 1.3 μg gallic acid equivalent/g). Data from LC-ESI-QTOF-MS allowed the identification of 21 compounds. The molecular docking study showed that 12 of the compounds could prevent tumors in breast cancer by acting as inhibitors of the anti-apoptotic HER2 protein. C. racemosa has potential in the chemoprevention of breast cancer through its radical scavenging capacity and inhibition of the HER2 protein. More studies are needed to investigate the efficacy of extracts in different models.http://www.sciencedirect.com/science/article/pii/S266615432300128XAnticancer propertiesBreast cancerFunctional foodGreen algaeHER2MCF-7 |
| spellingShingle | Grace Sanger Djuhria Wonggo Nurmelita Taher Verly Dotulong Aurielle Annalicia Setiawan Happy Kurnia Permatasari Sidik Maulana Fahrul Nurkolis Apollinaire Tsopmo Bonglee Kim Green seaweed Caulerpa racemosa - Chemical constituents, cytotoxicity in breast cancer cells and molecular docking simulation Journal of Agriculture and Food Research Anticancer properties Breast cancer Functional food Green algae HER2 MCF-7 |
| title | Green seaweed Caulerpa racemosa - Chemical constituents, cytotoxicity in breast cancer cells and molecular docking simulation |
| title_full | Green seaweed Caulerpa racemosa - Chemical constituents, cytotoxicity in breast cancer cells and molecular docking simulation |
| title_fullStr | Green seaweed Caulerpa racemosa - Chemical constituents, cytotoxicity in breast cancer cells and molecular docking simulation |
| title_full_unstemmed | Green seaweed Caulerpa racemosa - Chemical constituents, cytotoxicity in breast cancer cells and molecular docking simulation |
| title_short | Green seaweed Caulerpa racemosa - Chemical constituents, cytotoxicity in breast cancer cells and molecular docking simulation |
| title_sort | green seaweed caulerpa racemosa chemical constituents cytotoxicity in breast cancer cells and molecular docking simulation |
| topic | Anticancer properties Breast cancer Functional food Green algae HER2 MCF-7 |
| url | http://www.sciencedirect.com/science/article/pii/S266615432300128X |
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