Effects of Coverlys TF150<sup>®</sup> on the Photosynthetic Characteristics of Grape
Grape rain-shelter cultivation is a widely employed practice in China. At present, the most commonly used rain shelter film materials are polyvinyl chloride (PVC), polyethylene (PE), ethylene-vinyl acetate copolymer (EVA), and polyolefin (PO). Coverlys TF150<sup>®</sup> is a woven fabric...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/23/16659 |
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| author | Zhonghan Li Enshun Jiang Minghui Liu Qinghua Sun Zhen Gao Yuanpeng Du |
| author_facet | Zhonghan Li Enshun Jiang Minghui Liu Qinghua Sun Zhen Gao Yuanpeng Du |
| author_sort | Zhonghan Li |
| collection | DOAJ |
| description | Grape rain-shelter cultivation is a widely employed practice in China. At present, the most commonly used rain shelter film materials are polyvinyl chloride (PVC), polyethylene (PE), ethylene-vinyl acetate copolymer (EVA), and polyolefin (PO). Coverlys TF150<sup>®</sup> is a woven fabric with an internal antifoggy PE coating that has not yet been popularized as a rain shelter film for grapes in China. To investigate the effects of Coverlys TF150<sup>®</sup> on grapes, we measured the microdomain environment, leaf development, and photosynthetic characteristics of ‘Miguang’ (<i>Vitis vinifera</i> × <i>V. labrusca</i>) under rain-shelter cultivation and performed transcriptome analysis. The results showed that Coverlys TF150<sup>®</sup> significantly reduced (<i>p</i> < 0.05) the light intensity, temperature, and humidity compared with PO film, increased the chlorophyll content and leaf thickness (particularly palisade tissue thickness), and increased stomatal density and stomatal opening from 10:00 to 14:00. Coverlys TF150<sup>®</sup> was observed to improve the maximum efficiency of photosystem II (F<sub>v</sub>/F<sub>m</sub>), photochemical quenching (qP), the electron transfer rate (ETR), and the actual photochemical efficiency (Φ<sub>PSII</sub>) from 10:00 to 14:00. Moreover, the net photosynthetic rate (P<sub>n</sub>), intercellular CO<sub>2</sub> concentration (C<sub>i</sub>), stomatal conductance (G<sub>s</sub>), and transpiration rate (T<sub>r</sub>) of grape leaves significantly increased (<i>p</i> < 0.05) from 10:00 to 14:00. RNA-Seq analysis of the grape leaves at 8:00, 10:00, and 12:00 revealed 1388, 1562, and 1436 differential genes at these points in time, respectively. KEGG enrichment analysis showed the occurrence of protein processing in the endoplasmic reticulum. Plant hormone signal transduction and plant-pathogen interaction were identified as the metabolic pathways with the highest differential gene expression enrichment. The psbA encoding D1 protein was significantly up-regulated in both CO10vsPO10 and CO12vsPO12, while the sHSPs family genes were significantly down-regulated in all time periods, and thus may play an important role in the maintenance of the photosystem II (PSII) activity in grape leaves under Coverlys TF150<sup>®</sup>. Compared with PO film, the PSI-related gene psaB was up-regulated, indicating the ability of Coverlys TF150<sup>®</sup> to better maintain PSI activity. Compared with PO film, the abolic acid receptacle-associated gene PYL1 was down-regulated at all time periods under the Coverlys TF150<sup>®</sup> treatment, while PP2C47 was significantly up-regulated in CO10vsPO10 and CO12vsPO12, inducing stomatal closure. The results reveal that Coverlys TF150<sup>®</sup> alleviates the stress of high temperature and strong light compared with PO film, improves the photosynthetic capacity of grape leaves, and reduces the midday depression of photosynthesis. |
| first_indexed | 2024-03-09T01:50:57Z |
| format | Article |
| id | doaj.art-8cc93498ffff4e2abc54530e6759db43 |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T01:50:57Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-8cc93498ffff4e2abc54530e6759db432023-12-08T15:16:41ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-11-0124231665910.3390/ijms242316659Effects of Coverlys TF150<sup>®</sup> on the Photosynthetic Characteristics of GrapeZhonghan Li0Enshun Jiang1Minghui Liu2Qinghua Sun3Zhen Gao4Yuanpeng Du5College of Horticultural Science and Engineering, Shandong Agricultural University, Tai’an 271018, ChinaShandong Institute of Pomology, Tai’an 271000, ChinaCollege of Horticultural Science and Engineering, Shandong Agricultural University, Tai’an 271018, ChinaCollege of Horticultural Science and Engineering, Shandong Agricultural University, Tai’an 271018, ChinaCollege of Horticultural Science and Engineering, Shandong Agricultural University, Tai’an 271018, ChinaCollege of Horticultural Science and Engineering, Shandong Agricultural University, Tai’an 271018, ChinaGrape rain-shelter cultivation is a widely employed practice in China. At present, the most commonly used rain shelter film materials are polyvinyl chloride (PVC), polyethylene (PE), ethylene-vinyl acetate copolymer (EVA), and polyolefin (PO). Coverlys TF150<sup>®</sup> is a woven fabric with an internal antifoggy PE coating that has not yet been popularized as a rain shelter film for grapes in China. To investigate the effects of Coverlys TF150<sup>®</sup> on grapes, we measured the microdomain environment, leaf development, and photosynthetic characteristics of ‘Miguang’ (<i>Vitis vinifera</i> × <i>V. labrusca</i>) under rain-shelter cultivation and performed transcriptome analysis. The results showed that Coverlys TF150<sup>®</sup> significantly reduced (<i>p</i> < 0.05) the light intensity, temperature, and humidity compared with PO film, increased the chlorophyll content and leaf thickness (particularly palisade tissue thickness), and increased stomatal density and stomatal opening from 10:00 to 14:00. Coverlys TF150<sup>®</sup> was observed to improve the maximum efficiency of photosystem II (F<sub>v</sub>/F<sub>m</sub>), photochemical quenching (qP), the electron transfer rate (ETR), and the actual photochemical efficiency (Φ<sub>PSII</sub>) from 10:00 to 14:00. Moreover, the net photosynthetic rate (P<sub>n</sub>), intercellular CO<sub>2</sub> concentration (C<sub>i</sub>), stomatal conductance (G<sub>s</sub>), and transpiration rate (T<sub>r</sub>) of grape leaves significantly increased (<i>p</i> < 0.05) from 10:00 to 14:00. RNA-Seq analysis of the grape leaves at 8:00, 10:00, and 12:00 revealed 1388, 1562, and 1436 differential genes at these points in time, respectively. KEGG enrichment analysis showed the occurrence of protein processing in the endoplasmic reticulum. Plant hormone signal transduction and plant-pathogen interaction were identified as the metabolic pathways with the highest differential gene expression enrichment. The psbA encoding D1 protein was significantly up-regulated in both CO10vsPO10 and CO12vsPO12, while the sHSPs family genes were significantly down-regulated in all time periods, and thus may play an important role in the maintenance of the photosystem II (PSII) activity in grape leaves under Coverlys TF150<sup>®</sup>. Compared with PO film, the PSI-related gene psaB was up-regulated, indicating the ability of Coverlys TF150<sup>®</sup> to better maintain PSI activity. Compared with PO film, the abolic acid receptacle-associated gene PYL1 was down-regulated at all time periods under the Coverlys TF150<sup>®</sup> treatment, while PP2C47 was significantly up-regulated in CO10vsPO10 and CO12vsPO12, inducing stomatal closure. The results reveal that Coverlys TF150<sup>®</sup> alleviates the stress of high temperature and strong light compared with PO film, improves the photosynthetic capacity of grape leaves, and reduces the midday depression of photosynthesis.https://www.mdpi.com/1422-0067/24/23/16659grape (<i>Vitis vinifera</i>)rain shelter filmphotosynthesischlorophyll fluorescencetranscriptomedifferentially expressed gene |
| spellingShingle | Zhonghan Li Enshun Jiang Minghui Liu Qinghua Sun Zhen Gao Yuanpeng Du Effects of Coverlys TF150<sup>®</sup> on the Photosynthetic Characteristics of Grape International Journal of Molecular Sciences grape (<i>Vitis vinifera</i>) rain shelter film photosynthesis chlorophyll fluorescence transcriptome differentially expressed gene |
| title | Effects of Coverlys TF150<sup>®</sup> on the Photosynthetic Characteristics of Grape |
| title_full | Effects of Coverlys TF150<sup>®</sup> on the Photosynthetic Characteristics of Grape |
| title_fullStr | Effects of Coverlys TF150<sup>®</sup> on the Photosynthetic Characteristics of Grape |
| title_full_unstemmed | Effects of Coverlys TF150<sup>®</sup> on the Photosynthetic Characteristics of Grape |
| title_short | Effects of Coverlys TF150<sup>®</sup> on the Photosynthetic Characteristics of Grape |
| title_sort | effects of coverlys tf150 sup r sup on the photosynthetic characteristics of grape |
| topic | grape (<i>Vitis vinifera</i>) rain shelter film photosynthesis chlorophyll fluorescence transcriptome differentially expressed gene |
| url | https://www.mdpi.com/1422-0067/24/23/16659 |
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