Introgression of Seedling Plant Resistance to Leaf Rust from <i>Agropyron cristatum</i> into Wheat by Induced Homoeologous Recombination
<i>Agropyron cristatum</i> (P genome) is a Triticeae species from the wheat tertiary gene pool which has economic importance as forage and also displays traits beneficial to wheat. Resistance to leaf rust was previously mapped to the short arm of chromosome 1P (1PS) in <i>A. crista...
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| Format: | Article |
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MDPI AG
2023-01-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/13/2/334 |
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| author | Adoración Cabrera Rafael Porras Carmen Palomino Josefina Carmen Sillero |
| author_facet | Adoración Cabrera Rafael Porras Carmen Palomino Josefina Carmen Sillero |
| author_sort | Adoración Cabrera |
| collection | DOAJ |
| description | <i>Agropyron cristatum</i> (P genome) is a Triticeae species from the wheat tertiary gene pool which has economic importance as forage and also displays traits beneficial to wheat. Resistance to leaf rust was previously mapped to the short arm of chromosome 1P (1PS) in <i>A. cristatum</i> by the development of a compensating Robertsonian translocation involving chromosome arm 1PS and the long arm of wheat chromosome 1B (1BL). In this study, chromosome arm 1PS was engineered using the <i>ph1b</i> mutation to induce 1BS/1PS homoeologous recombination and to obtain new translocations with shortened fragments of chromosome arm 1PS. Two translocations with different alien fragment sizes were identified by genomic in situ hybridization, wheat 1BS- and 1PS-specific molecular markers and gene-specific markers for glutenin, <i>Glu-B3</i> and gliadin <i>Gli-B1</i> seed storage protein. One translocation (called type 1) replaces a proximal segment of 1PS chromatin, and the other (called type 2) replaces a distal 1PS segment and introduces the <i>Glu-B3</i> and <i>Gli-B1</i> wheat storage protein loci. Six specific EST-STS markers for chromosome arm 1PS amplified PCR products in the recombinant type 2 translocation line. Resistance analysis showed that the type 2 translocation was highly resistant to a virulent race of leaf rust pathogen. The new wheat–<i>A. cristatum</i> translocations obtained yield material with seedling plant resistance to leaf rust and seed storage protein loci. |
| first_indexed | 2024-03-11T09:17:06Z |
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| id | doaj.art-8cca6b4b0c8d4652aba067666cde5a3c |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-11T09:17:06Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-8cca6b4b0c8d4652aba067666cde5a3c2023-11-16T18:33:05ZengMDPI AGAgronomy2073-43952023-01-0113233410.3390/agronomy13020334Introgression of Seedling Plant Resistance to Leaf Rust from <i>Agropyron cristatum</i> into Wheat by Induced Homoeologous RecombinationAdoración Cabrera0Rafael Porras1Carmen Palomino2Josefina Carmen Sillero3Departamento de Genética, Escuela Técnica Superior de Ingeniería Agronómica y de Montes, Edificio Gregor Mendel, Campus de Rabanales, Universidad de Córdoba, CeiA3, 14071 Córdoba, SpainInstituto de Investigación y Formación Agraria y Pesquera, (IFAPA) Alameda del Obispo, Area of Plant Breeding and Biotechnology, Avda. Menéndez Pidal s/n, 14004 Córdoba, SpainDepartamento de Genética, Escuela Técnica Superior de Ingeniería Agronómica y de Montes, Edificio Gregor Mendel, Campus de Rabanales, Universidad de Córdoba, CeiA3, 14071 Córdoba, SpainInstituto de Investigación y Formación Agraria y Pesquera, (IFAPA) Alameda del Obispo, Area of Plant Breeding and Biotechnology, Avda. Menéndez Pidal s/n, 14004 Córdoba, Spain<i>Agropyron cristatum</i> (P genome) is a Triticeae species from the wheat tertiary gene pool which has economic importance as forage and also displays traits beneficial to wheat. Resistance to leaf rust was previously mapped to the short arm of chromosome 1P (1PS) in <i>A. cristatum</i> by the development of a compensating Robertsonian translocation involving chromosome arm 1PS and the long arm of wheat chromosome 1B (1BL). In this study, chromosome arm 1PS was engineered using the <i>ph1b</i> mutation to induce 1BS/1PS homoeologous recombination and to obtain new translocations with shortened fragments of chromosome arm 1PS. Two translocations with different alien fragment sizes were identified by genomic in situ hybridization, wheat 1BS- and 1PS-specific molecular markers and gene-specific markers for glutenin, <i>Glu-B3</i> and gliadin <i>Gli-B1</i> seed storage protein. One translocation (called type 1) replaces a proximal segment of 1PS chromatin, and the other (called type 2) replaces a distal 1PS segment and introduces the <i>Glu-B3</i> and <i>Gli-B1</i> wheat storage protein loci. Six specific EST-STS markers for chromosome arm 1PS amplified PCR products in the recombinant type 2 translocation line. Resistance analysis showed that the type 2 translocation was highly resistant to a virulent race of leaf rust pathogen. The new wheat–<i>A. cristatum</i> translocations obtained yield material with seedling plant resistance to leaf rust and seed storage protein loci.https://www.mdpi.com/2073-4395/13/2/334<i>Agropyron cristatum</i>homoeologous pairingleaf rust resistancewheat |
| spellingShingle | Adoración Cabrera Rafael Porras Carmen Palomino Josefina Carmen Sillero Introgression of Seedling Plant Resistance to Leaf Rust from <i>Agropyron cristatum</i> into Wheat by Induced Homoeologous Recombination Agronomy <i>Agropyron cristatum</i> homoeologous pairing leaf rust resistance wheat |
| title | Introgression of Seedling Plant Resistance to Leaf Rust from <i>Agropyron cristatum</i> into Wheat by Induced Homoeologous Recombination |
| title_full | Introgression of Seedling Plant Resistance to Leaf Rust from <i>Agropyron cristatum</i> into Wheat by Induced Homoeologous Recombination |
| title_fullStr | Introgression of Seedling Plant Resistance to Leaf Rust from <i>Agropyron cristatum</i> into Wheat by Induced Homoeologous Recombination |
| title_full_unstemmed | Introgression of Seedling Plant Resistance to Leaf Rust from <i>Agropyron cristatum</i> into Wheat by Induced Homoeologous Recombination |
| title_short | Introgression of Seedling Plant Resistance to Leaf Rust from <i>Agropyron cristatum</i> into Wheat by Induced Homoeologous Recombination |
| title_sort | introgression of seedling plant resistance to leaf rust from i agropyron cristatum i into wheat by induced homoeologous recombination |
| topic | <i>Agropyron cristatum</i> homoeologous pairing leaf rust resistance wheat |
| url | https://www.mdpi.com/2073-4395/13/2/334 |
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