Expression analyses of some Beauveria bassiana genes in response to cuticles of four different insects
Beauveria spp. as entomopathogenic fungi have extremely diverse insect host ranges.Here, a comparative transcript analysis of a Bassiasin I (BSNI), a Dipeptidyl peptidase (DPP), a Cytochrom P450 monooxygenase (CYP52), a Mitogen activated protein kinase (MAPK), and a Neuronal calcium sensor 1 (NCS) i...
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| Format: | Article |
| Language: | English |
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University of Tarbiat Modares
2015-12-01
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| Series: | Journal of Crop Protection |
| Subjects: | |
| Online Access: | http://jcp.modares.ac.ir/article-3-3677-en.html |
| _version_ | 1811256579123576832 |
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| author | Masoumeh Kordi Naser Farrokhi Abolfazl Masoudi Ali Derakhshan Shadmehri Shahrokh Gharanjik |
| author_facet | Masoumeh Kordi Naser Farrokhi Abolfazl Masoudi Ali Derakhshan Shadmehri Shahrokh Gharanjik |
| author_sort | Masoumeh Kordi |
| collection | DOAJ |
| description | Beauveria spp. as entomopathogenic fungi have extremely diverse insect host ranges.Here, a comparative transcript analysis of a Bassiasin I (BSNI), a Dipeptidyl peptidase (DPP), a Cytochrom P450 monooxygenase (CYP52), a Mitogen activated protein kinase (MAPK), and a Neuronal calcium sensor 1 (NCS) in two isolates of Beauveria bassiana., with low and high virulence, were carried out. For this, induction media, containing isolated cuticles from four insects, and a check medium lacking any cuticular extract were prepared and isolates were allowed to grow on them. Interestingly, up-regulation of BSNI and DPP in induction media was noted in comparison to the check, following normalization with γ-ACTIN. CYP52 was up-regulated in synthetic medium in comparison to cuticular extract. MAPK expression was greater in most virulent isolate once compared with the low virulent isolate. The expression of MAPK was greater in the presence of Caliptamus italicus cuticles and synthetic medium as opposed to others. NCS expression remained on changed in all media tested and can be suggested as a reference gene in transcriptomics of Beauveria spp. genes in future. |
| first_indexed | 2024-04-12T17:43:35Z |
| format | Article |
| id | doaj.art-8cca89ebbc1046ebbf8135f0ba69ed82 |
| institution | Directory Open Access Journal |
| issn | 2251-9041 2251-905X |
| language | English |
| last_indexed | 2024-04-12T17:43:35Z |
| publishDate | 2015-12-01 |
| publisher | University of Tarbiat Modares |
| record_format | Article |
| series | Journal of Crop Protection |
| spelling | doaj.art-8cca89ebbc1046ebbf8135f0ba69ed822022-12-22T03:22:44ZengUniversity of Tarbiat ModaresJournal of Crop Protection2251-90412251-905X2015-12-0145675690Expression analyses of some Beauveria bassiana genes in response to cuticles of four different insectsMasoumeh Kordi0Naser Farrokhi1Abolfazl Masoudi2Ali Derakhshan Shadmehri3Shahrokh Gharanjik4 Biotechnology Research Department, Shahrood University, Shahrood, Iran. Department of Biotechnology Engineering, Faculty of Energy Engineering and New Technologies, Shahid Beheshti University, Tehran, Iran. Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahrood University, Sahrood, Iran. Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahrood University, Sahrood, Iran. Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahrood University, Sahrood, Iran. Beauveria spp. as entomopathogenic fungi have extremely diverse insect host ranges.Here, a comparative transcript analysis of a Bassiasin I (BSNI), a Dipeptidyl peptidase (DPP), a Cytochrom P450 monooxygenase (CYP52), a Mitogen activated protein kinase (MAPK), and a Neuronal calcium sensor 1 (NCS) in two isolates of Beauveria bassiana., with low and high virulence, were carried out. For this, induction media, containing isolated cuticles from four insects, and a check medium lacking any cuticular extract were prepared and isolates were allowed to grow on them. Interestingly, up-regulation of BSNI and DPP in induction media was noted in comparison to the check, following normalization with γ-ACTIN. CYP52 was up-regulated in synthetic medium in comparison to cuticular extract. MAPK expression was greater in most virulent isolate once compared with the low virulent isolate. The expression of MAPK was greater in the presence of Caliptamus italicus cuticles and synthetic medium as opposed to others. NCS expression remained on changed in all media tested and can be suggested as a reference gene in transcriptomics of Beauveria spp. genes in future.http://jcp.modares.ac.ir/article-3-3677-en.htmlbassiasin idipeptidyl peptidasecytochrome p450 monooxygenasemitogen activated protein kinaseneuronal calcium sensor |
| spellingShingle | Masoumeh Kordi Naser Farrokhi Abolfazl Masoudi Ali Derakhshan Shadmehri Shahrokh Gharanjik Expression analyses of some Beauveria bassiana genes in response to cuticles of four different insects Journal of Crop Protection bassiasin i dipeptidyl peptidase cytochrome p450 monooxygenase mitogen activated protein kinase neuronal calcium sensor |
| title | Expression analyses of some Beauveria bassiana genes in response to cuticles of four different insects |
| title_full | Expression analyses of some Beauveria bassiana genes in response to cuticles of four different insects |
| title_fullStr | Expression analyses of some Beauveria bassiana genes in response to cuticles of four different insects |
| title_full_unstemmed | Expression analyses of some Beauveria bassiana genes in response to cuticles of four different insects |
| title_short | Expression analyses of some Beauveria bassiana genes in response to cuticles of four different insects |
| title_sort | expression analyses of some beauveria bassiana genes in response to cuticles of four different insects |
| topic | bassiasin i dipeptidyl peptidase cytochrome p450 monooxygenase mitogen activated protein kinase neuronal calcium sensor |
| url | http://jcp.modares.ac.ir/article-3-3677-en.html |
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