Activation of human erythrocyte glutathione – s – transferase (EC. 2.5.1.18) by caffeine (1, 3, 7 – trimethylxanthine)
Caffeine (1,3,7 – trimethylxanthine) was extracted from Nescafe ® (product number CC5AA) a brand of “instant coffees” produced by Nestle foods, Lagos, Nigeria, using Harris method adapted from FDALS (1982) and confirmed by Wagenar test (Arnand, 1984). Various concentrations (5.0mM, 10.0mM, 15.0mM, 2...
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| Format: | Article |
| Language: | English |
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Joint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP)
2004-03-01
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| Series: | Journal of Applied Sciences and Environmental Management |
| Online Access: | https://www.ajol.info/index.php/jasem/article/view/17210 |
| _version_ | 1797227844057694208 |
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| author | A.I. SPIFF A.A. UWAKWE |
| author_facet | A.I. SPIFF A.A. UWAKWE |
| author_sort | A.I. SPIFF |
| collection | DOAJ |
| description | Caffeine (1,3,7 – trimethylxanthine) was extracted from Nescafe ® (product number CC5AA) a brand of “instant coffees” produced by Nestle foods, Lagos, Nigeria, using Harris method adapted from FDALS (1982) and confirmed by Wagenar test (Arnand, 1984). Various concentrations (5.0mM, 10.0mM, 15.0mM, 20.0mM, 25.0mM and 50.0mM) of the isolated caffeine were tested in-vitro on their possible effect on human erythrocyte (red cell) glutathione – S – transferase (EC. 2.5.1.18) activity. The result indicated significant (P < 0.05) activation of the erythrocyte enzyme (GST) by caffeine in a concentration – dependent manner i.e. higher concentrations produced greater activation of the enzyme. For instance at caffeine concentrations of 5.0mM, 25.0mM and 50.0mM, red cell GST activity was increased by 1.99 folds (299.43%), 4.99 folds (598.85%) and 6.98 folds (798.28%) respectively. These results may point to a possible binding – deterioration of caffeine by the human erythrocyte GST.
Journal of Applied Sciences and Environmental Management Vol. 7(2) 2003: 45-48 |
| first_indexed | 2024-04-24T14:47:15Z |
| format | Article |
| id | doaj.art-8cd210bc4e7d4ebbb69eb245175d8f84 |
| institution | Directory Open Access Journal |
| issn | 2659-1502 2659-1499 |
| language | English |
| last_indexed | 2024-04-24T14:47:15Z |
| publishDate | 2004-03-01 |
| publisher | Joint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP) |
| record_format | Article |
| series | Journal of Applied Sciences and Environmental Management |
| spelling | doaj.art-8cd210bc4e7d4ebbb69eb245175d8f842024-04-02T19:55:03ZengJoint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP)Journal of Applied Sciences and Environmental Management2659-15022659-14992004-03-017210.4314/jasem.v7i2.17210Activation of human erythrocyte glutathione – s – transferase (EC. 2.5.1.18) by caffeine (1, 3, 7 – trimethylxanthine)A.I. SPIFF0A.A. UWAKWE1Department of Chemistry, University of Port Harcourt, Port Harcourt, Nigeria.Department of Biochemistry, University of Port Harcourt, Port Harcourt, NigeriaCaffeine (1,3,7 – trimethylxanthine) was extracted from Nescafe ® (product number CC5AA) a brand of “instant coffees” produced by Nestle foods, Lagos, Nigeria, using Harris method adapted from FDALS (1982) and confirmed by Wagenar test (Arnand, 1984). Various concentrations (5.0mM, 10.0mM, 15.0mM, 20.0mM, 25.0mM and 50.0mM) of the isolated caffeine were tested in-vitro on their possible effect on human erythrocyte (red cell) glutathione – S – transferase (EC. 2.5.1.18) activity. The result indicated significant (P < 0.05) activation of the erythrocyte enzyme (GST) by caffeine in a concentration – dependent manner i.e. higher concentrations produced greater activation of the enzyme. For instance at caffeine concentrations of 5.0mM, 25.0mM and 50.0mM, red cell GST activity was increased by 1.99 folds (299.43%), 4.99 folds (598.85%) and 6.98 folds (798.28%) respectively. These results may point to a possible binding – deterioration of caffeine by the human erythrocyte GST. Journal of Applied Sciences and Environmental Management Vol. 7(2) 2003: 45-48https://www.ajol.info/index.php/jasem/article/view/17210 |
| spellingShingle | A.I. SPIFF A.A. UWAKWE Activation of human erythrocyte glutathione – s – transferase (EC. 2.5.1.18) by caffeine (1, 3, 7 – trimethylxanthine) Journal of Applied Sciences and Environmental Management |
| title | Activation of human erythrocyte glutathione – s – transferase (EC. 2.5.1.18) by caffeine (1, 3, 7 – trimethylxanthine) |
| title_full | Activation of human erythrocyte glutathione – s – transferase (EC. 2.5.1.18) by caffeine (1, 3, 7 – trimethylxanthine) |
| title_fullStr | Activation of human erythrocyte glutathione – s – transferase (EC. 2.5.1.18) by caffeine (1, 3, 7 – trimethylxanthine) |
| title_full_unstemmed | Activation of human erythrocyte glutathione – s – transferase (EC. 2.5.1.18) by caffeine (1, 3, 7 – trimethylxanthine) |
| title_short | Activation of human erythrocyte glutathione – s – transferase (EC. 2.5.1.18) by caffeine (1, 3, 7 – trimethylxanthine) |
| title_sort | activation of human erythrocyte glutathione s transferase ec 2 5 1 18 by caffeine 1 3 7 trimethylxanthine |
| url | https://www.ajol.info/index.php/jasem/article/view/17210 |
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