Soil microbial improvement using enriched vinasse as a new abundant waste
Abstract This study proposes the use of vinasse, an inexpensive and readily available waste biopolymer, as a fundamental component of a waste culture medium that can enhance the effectiveness and cost-efficiency of the microbial-induced calcite precipitation (MICP) method for sustainable soil improv...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-12-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-49401-w |
| _version_ | 1797388353688043520 |
|---|---|
| author | Tahereh Kariminia Mohammad A. Rowshanzamir S. Mahdi Abtahi Sabihe Soleimanian-Zad Hamid Mortazavi Bak Alireza Baghbanan |
| author_facet | Tahereh Kariminia Mohammad A. Rowshanzamir S. Mahdi Abtahi Sabihe Soleimanian-Zad Hamid Mortazavi Bak Alireza Baghbanan |
| author_sort | Tahereh Kariminia |
| collection | DOAJ |
| description | Abstract This study proposes the use of vinasse, an inexpensive and readily available waste biopolymer, as a fundamental component of a waste culture medium that can enhance the effectiveness and cost-efficiency of the microbial-induced calcite precipitation (MICP) method for sustainable soil improvement. Vinasse enriched with urea, sodium caseinate, or whey protein concentrate is employed to optimize bacterial growth and urease activity of Sporosarcina pasteurii (S. pasteurii) bacterium. The best culture medium is analyzed using Taguchi design of experiments (TDOE) and statistical analysis, considering the concentration of vinasse and urea as effective parameters during growth time. To test the best culture medium for bio-treated soil, direct shear tests were performed on loose and bio-treated sand. The results demonstrate a substantial cost reduction from $0.455 to $0.005 per liter when using the new culture medium (vinasse and urea) compared to the conventional Nutrient Broth (NB) culture medium. Additionally, the new medium enhances soil shear strength, increasing the friction angle by 2.5 degrees and cohesion to 20.7 kPa compared to the conventional medium. Furthermore, the recycling of vinasse as a waste product can promote the progress of a circular economy and reduce environmental pollution. As ground improvement is essential for many construction projects, especially those that require high shear strength or are built on loose soil, this study provides a promising approach to achieving cost-effective and sustainable soil microbial improvement using enriched vinasse. |
| first_indexed | 2024-03-08T22:39:32Z |
| format | Article |
| id | doaj.art-8cd482616c264fdca3c0f9b9d5e0cf3c |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-08T22:39:32Z |
| publishDate | 2023-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-8cd482616c264fdca3c0f9b9d5e0cf3c2023-12-17T12:15:16ZengNature PortfolioScientific Reports2045-23222023-12-0113111510.1038/s41598-023-49401-wSoil microbial improvement using enriched vinasse as a new abundant wasteTahereh Kariminia0Mohammad A. Rowshanzamir1S. Mahdi Abtahi2Sabihe Soleimanian-Zad3Hamid Mortazavi Bak4Alireza Baghbanan5Department of Civil Engineering, Isfahan University of TechnologyDepartment of Civil Engineering, Isfahan University of TechnologyDepartment of Civil Engineering, Isfahan University of TechnologyDepartment of Food Science and Technology, College of Agriculture, Isfahan University of TechnologySchool of Civil and Environmental Engineering, University of New South WalesDepartment of Mining Engineering, Isfahan University of TechnologyAbstract This study proposes the use of vinasse, an inexpensive and readily available waste biopolymer, as a fundamental component of a waste culture medium that can enhance the effectiveness and cost-efficiency of the microbial-induced calcite precipitation (MICP) method for sustainable soil improvement. Vinasse enriched with urea, sodium caseinate, or whey protein concentrate is employed to optimize bacterial growth and urease activity of Sporosarcina pasteurii (S. pasteurii) bacterium. The best culture medium is analyzed using Taguchi design of experiments (TDOE) and statistical analysis, considering the concentration of vinasse and urea as effective parameters during growth time. To test the best culture medium for bio-treated soil, direct shear tests were performed on loose and bio-treated sand. The results demonstrate a substantial cost reduction from $0.455 to $0.005 per liter when using the new culture medium (vinasse and urea) compared to the conventional Nutrient Broth (NB) culture medium. Additionally, the new medium enhances soil shear strength, increasing the friction angle by 2.5 degrees and cohesion to 20.7 kPa compared to the conventional medium. Furthermore, the recycling of vinasse as a waste product can promote the progress of a circular economy and reduce environmental pollution. As ground improvement is essential for many construction projects, especially those that require high shear strength or are built on loose soil, this study provides a promising approach to achieving cost-effective and sustainable soil microbial improvement using enriched vinasse.https://doi.org/10.1038/s41598-023-49401-w |
| spellingShingle | Tahereh Kariminia Mohammad A. Rowshanzamir S. Mahdi Abtahi Sabihe Soleimanian-Zad Hamid Mortazavi Bak Alireza Baghbanan Soil microbial improvement using enriched vinasse as a new abundant waste Scientific Reports |
| title | Soil microbial improvement using enriched vinasse as a new abundant waste |
| title_full | Soil microbial improvement using enriched vinasse as a new abundant waste |
| title_fullStr | Soil microbial improvement using enriched vinasse as a new abundant waste |
| title_full_unstemmed | Soil microbial improvement using enriched vinasse as a new abundant waste |
| title_short | Soil microbial improvement using enriched vinasse as a new abundant waste |
| title_sort | soil microbial improvement using enriched vinasse as a new abundant waste |
| url | https://doi.org/10.1038/s41598-023-49401-w |
| work_keys_str_mv | AT taherehkariminia soilmicrobialimprovementusingenrichedvinasseasanewabundantwaste AT mohammadarowshanzamir soilmicrobialimprovementusingenrichedvinasseasanewabundantwaste AT smahdiabtahi soilmicrobialimprovementusingenrichedvinasseasanewabundantwaste AT sabihesoleimanianzad soilmicrobialimprovementusingenrichedvinasseasanewabundantwaste AT hamidmortazavibak soilmicrobialimprovementusingenrichedvinasseasanewabundantwaste AT alirezabaghbanan soilmicrobialimprovementusingenrichedvinasseasanewabundantwaste |