IN SILICO ANALYSIS AND OVARY FATTY ACID COMPOSITION OF COBIA FISH (RACHYCENTRON CANADUM)
There has been not much information available related to the diet formulation for female Cobia (Rachycentron canadum) fish. This study characterized ovary fatty acid composition of female Cobia in their different gonad maturation stages and predicted the mechanism of HUFA (DHA/docosahexaenoic acid,...
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| Format: | Article |
| Language: | English |
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iVolga Press
2020-07-01
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| Series: | Russian Journal of Agricultural and Socio-Economic Sciences |
| Subjects: | |
| Online Access: | https://rjoas.com/issue-2020-07/article_21.pdf |
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| author | Asmanik Nursyam H. Widodo M.S. Ekawati A.W. Riyadi P.H. Amin M. |
| author_facet | Asmanik Nursyam H. Widodo M.S. Ekawati A.W. Riyadi P.H. Amin M. |
| author_sort | Asmanik |
| collection | DOAJ |
| description | There has been not much information available related to the diet formulation for female Cobia (Rachycentron canadum) fish. This study characterized ovary fatty acid composition of female Cobia in their different gonad maturation stages and predicted the mechanism of HUFA (DHA/docosahexaenoic acid, EPA/eicosapentaenoic acid, and AA/arachidonic acid) in supporting gonad maturation through an in silico analysis. Fish samples consisted of three (3) categories: (1) prospective broodstocks – immature (initial development); (2) prospective broodstocks – maturing (middle development); and (3) broodstocks – mature (advanced development). The in silico analysis was done through a molecular docking between ligand (DHA, EPA, and AA) and receptors Lpl, Fatp, Fabp, and Mttp. Our findings showed that fatty acids tended to increase along with the development of the ovary. The highest fatty acids for group 1 was C16:0>C18:1n9c>DHA, for group 2 was C18:1n9c>C16:0>DHA, and for group 3 was DHA>C16:0>C18:1n9c. DHA increased significantly during gonad maturation and the vice versa happened to EPA, while AA increased significantly at the end of gonad maturation. This affected, among others, the ratio of DHA/EPA and DHA/AA—DHA/EPA ration increased along with gonad maturation, while the opposite happened for DHA/AA ratio. The results of the in silico analysis showed that DHA had the smallest binding affinity for docking with Lpl, Fatp, and Fapb compared to EPA and AA. This means that DHA was predicted to have a strong binding with receptors during the oocyte lipidation. DHA also had the smallest binding affinity for docking with Mttp compared to EPA and AA. This means that DHA was predicted to have a strong binding with receptors in Vtg lipidation compared to EPA and AA. |
| first_indexed | 2024-03-12T18:09:30Z |
| format | Article |
| id | doaj.art-8cd545ae6902490c88bd94bf20b016da |
| institution | Directory Open Access Journal |
| issn | 2226-1184 |
| language | English |
| last_indexed | 2024-03-12T18:09:30Z |
| publishDate | 2020-07-01 |
| publisher | iVolga Press |
| record_format | Article |
| series | Russian Journal of Agricultural and Socio-Economic Sciences |
| spelling | doaj.art-8cd545ae6902490c88bd94bf20b016da2023-08-02T09:17:03ZengiVolga PressRussian Journal of Agricultural and Socio-Economic Sciences2226-11842020-07-01103717017810.18551/rjoas.2020-07.21IN SILICO ANALYSIS AND OVARY FATTY ACID COMPOSITION OF COBIA FISH (RACHYCENTRON CANADUM)Asmanik0Nursyam H.1Widodo M.S.2Ekawati A.W.3Riyadi P.H.4Amin M.5Faculty of Fisheries and Marine Science, University of BrawijayaFaculty of Fisheries and Marine Science, University of BrawijayaFaculty of Fisheries and Marine Science, University of BrawijayaFaculty of Fisheries and Marine Science, University of BrawijayaFaculty of Fisheries and Marine Science, University of DiponegoroDepartment of Biology, Faculty of Mathematics and Natural Sciences, State University of MalangThere has been not much information available related to the diet formulation for female Cobia (Rachycentron canadum) fish. This study characterized ovary fatty acid composition of female Cobia in their different gonad maturation stages and predicted the mechanism of HUFA (DHA/docosahexaenoic acid, EPA/eicosapentaenoic acid, and AA/arachidonic acid) in supporting gonad maturation through an in silico analysis. Fish samples consisted of three (3) categories: (1) prospective broodstocks – immature (initial development); (2) prospective broodstocks – maturing (middle development); and (3) broodstocks – mature (advanced development). The in silico analysis was done through a molecular docking between ligand (DHA, EPA, and AA) and receptors Lpl, Fatp, Fabp, and Mttp. Our findings showed that fatty acids tended to increase along with the development of the ovary. The highest fatty acids for group 1 was C16:0>C18:1n9c>DHA, for group 2 was C18:1n9c>C16:0>DHA, and for group 3 was DHA>C16:0>C18:1n9c. DHA increased significantly during gonad maturation and the vice versa happened to EPA, while AA increased significantly at the end of gonad maturation. This affected, among others, the ratio of DHA/EPA and DHA/AA—DHA/EPA ration increased along with gonad maturation, while the opposite happened for DHA/AA ratio. The results of the in silico analysis showed that DHA had the smallest binding affinity for docking with Lpl, Fatp, and Fapb compared to EPA and AA. This means that DHA was predicted to have a strong binding with receptors during the oocyte lipidation. DHA also had the smallest binding affinity for docking with Mttp compared to EPA and AA. This means that DHA was predicted to have a strong binding with receptors in Vtg lipidation compared to EPA and AA.https://rjoas.com/issue-2020-07/article_21.pdfcobia (rachycentron canadum)fatty acidovaryin silico |
| spellingShingle | Asmanik Nursyam H. Widodo M.S. Ekawati A.W. Riyadi P.H. Amin M. IN SILICO ANALYSIS AND OVARY FATTY ACID COMPOSITION OF COBIA FISH (RACHYCENTRON CANADUM) Russian Journal of Agricultural and Socio-Economic Sciences cobia (rachycentron canadum) fatty acid ovary in silico |
| title | IN SILICO ANALYSIS AND OVARY FATTY ACID COMPOSITION OF COBIA FISH (RACHYCENTRON CANADUM) |
| title_full | IN SILICO ANALYSIS AND OVARY FATTY ACID COMPOSITION OF COBIA FISH (RACHYCENTRON CANADUM) |
| title_fullStr | IN SILICO ANALYSIS AND OVARY FATTY ACID COMPOSITION OF COBIA FISH (RACHYCENTRON CANADUM) |
| title_full_unstemmed | IN SILICO ANALYSIS AND OVARY FATTY ACID COMPOSITION OF COBIA FISH (RACHYCENTRON CANADUM) |
| title_short | IN SILICO ANALYSIS AND OVARY FATTY ACID COMPOSITION OF COBIA FISH (RACHYCENTRON CANADUM) |
| title_sort | in silico analysis and ovary fatty acid composition of cobia fish rachycentron canadum |
| topic | cobia (rachycentron canadum) fatty acid ovary in silico |
| url | https://rjoas.com/issue-2020-07/article_21.pdf |
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