Anti-Influenza Virus Potential of Probiotic Strain <i>Lactoplantibacillus plantarum</i> YML015 Isolated from Korean Fermented Vegetable
Lactic acid bacteria are one of the potential natural remedies used worldwide, commonly known as probiotics. Here, the aim of this research investigation was to isolate a probiotic <i>Lactobacilli</i> strain, YLM015, from the popular Korean fermented vegetable “Kimchi” and to evaluate it...
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MDPI AG
2022-10-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/8/11/572 |
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| author | Rajib Majumder Md Badrul Alam Keshav Raj Paudel Khandaker Asif Ahmed Hari Prasad Devkota Sang-Han Lee Philip M. Hansbro Yong-Ha Park |
| author_facet | Rajib Majumder Md Badrul Alam Keshav Raj Paudel Khandaker Asif Ahmed Hari Prasad Devkota Sang-Han Lee Philip M. Hansbro Yong-Ha Park |
| author_sort | Rajib Majumder |
| collection | DOAJ |
| description | Lactic acid bacteria are one of the potential natural remedies used worldwide, commonly known as probiotics. Here, the aim of this research investigation was to isolate a probiotic <i>Lactobacilli</i> strain, YLM015, from the popular Korean fermented vegetable “Kimchi” and to evaluate its anti-viral potential against influenza virus A (IFVA) H1N1 using the MDCK cell line in vitro, and in embryonated eggs in ovo. The YML015 strain was selected from among the 1200 <i>Lactobacilli</i> isolates for further studies based on its potent anti-viral efficacy. YML015 was identified and characterized as <i>Lactoplantibacillus plantarum</i> YML015 based on the 16S rRNA gene sequencing and biochemically with an API 50 CHL Kit. In ovo assay experienced with embryonated eggs and the hemagglutination inhibition method, as well as cytopathogenic reduction assay, was performed individually to observe anti-influenza viral activity of YML015 against influenza virus A H1N1. Additionally, YML015 was classified for its non-resistance nature as safe for humans and animals as confirmed by the antibiotic susceptibility (MIC) test, cell viability, and hemolysis assay. The heat stability test was also experienced by using different heat-treated cell-free supernatant (CFS) samples of YML015. As a result, YML015 showed highly potent anti-viral activity against influenza virus A H1N1 in vitro in the MDCK cell line. Overall findings suggest that anti-influenza viral activity of <i>L. plantarum</i> YML015 makes it a potential candidate of choice for use as an influential probiotic in pharmacological preparations to protect humans and animals from flu and viral infection. |
| first_indexed | 2024-03-09T19:05:36Z |
| format | Article |
| id | doaj.art-8cd595e7708d44659cdbb68f4f53caaa |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-09T19:05:36Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-8cd595e7708d44659cdbb68f4f53caaa2023-11-24T04:37:38ZengMDPI AGFermentation2311-56372022-10-0181157210.3390/fermentation8110572Anti-Influenza Virus Potential of Probiotic Strain <i>Lactoplantibacillus plantarum</i> YML015 Isolated from Korean Fermented VegetableRajib Majumder0Md Badrul Alam1Keshav Raj Paudel2Khandaker Asif Ahmed3Hari Prasad Devkota4Sang-Han Lee5Philip M. Hansbro6Yong-Ha Park7Centre for Inflammation, Centenary Institute and University of Technology Sydney, Faculty of Science, School of Life Sciences, Sydney, NSW 2007, AustraliaDepartment of Food Science and Biotechnology, Graduate School, Kyunpook National University, Daegu 37224, KoreaCentre for Inflammation, Centenary Institute and University of Technology Sydney, Faculty of Science, School of Life Sciences, Sydney, NSW 2007, AustraliaApplied Biosciences, Macquarie University, Sydney, NSW 2109, AustraliaGraduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto 862-0973, JapanDepartment of Food Science and Biotechnology, Graduate School, Kyunpook National University, Daegu 37224, KoreaCentre for Inflammation, Centenary Institute and University of Technology Sydney, Faculty of Science, School of Life Sciences, Sydney, NSW 2007, AustraliaDepartment of Applied Microbiology and Biotechnology, Yeungnam University, Gyeongsan 38541, KoreaLactic acid bacteria are one of the potential natural remedies used worldwide, commonly known as probiotics. Here, the aim of this research investigation was to isolate a probiotic <i>Lactobacilli</i> strain, YLM015, from the popular Korean fermented vegetable “Kimchi” and to evaluate its anti-viral potential against influenza virus A (IFVA) H1N1 using the MDCK cell line in vitro, and in embryonated eggs in ovo. The YML015 strain was selected from among the 1200 <i>Lactobacilli</i> isolates for further studies based on its potent anti-viral efficacy. YML015 was identified and characterized as <i>Lactoplantibacillus plantarum</i> YML015 based on the 16S rRNA gene sequencing and biochemically with an API 50 CHL Kit. In ovo assay experienced with embryonated eggs and the hemagglutination inhibition method, as well as cytopathogenic reduction assay, was performed individually to observe anti-influenza viral activity of YML015 against influenza virus A H1N1. Additionally, YML015 was classified for its non-resistance nature as safe for humans and animals as confirmed by the antibiotic susceptibility (MIC) test, cell viability, and hemolysis assay. The heat stability test was also experienced by using different heat-treated cell-free supernatant (CFS) samples of YML015. As a result, YML015 showed highly potent anti-viral activity against influenza virus A H1N1 in vitro in the MDCK cell line. Overall findings suggest that anti-influenza viral activity of <i>L. plantarum</i> YML015 makes it a potential candidate of choice for use as an influential probiotic in pharmacological preparations to protect humans and animals from flu and viral infection.https://www.mdpi.com/2311-5637/8/11/572probiotic<i>L. plantarum</i> YML015antiviral activity |
| spellingShingle | Rajib Majumder Md Badrul Alam Keshav Raj Paudel Khandaker Asif Ahmed Hari Prasad Devkota Sang-Han Lee Philip M. Hansbro Yong-Ha Park Anti-Influenza Virus Potential of Probiotic Strain <i>Lactoplantibacillus plantarum</i> YML015 Isolated from Korean Fermented Vegetable Fermentation probiotic <i>L. plantarum</i> YML015 antiviral activity |
| title | Anti-Influenza Virus Potential of Probiotic Strain <i>Lactoplantibacillus plantarum</i> YML015 Isolated from Korean Fermented Vegetable |
| title_full | Anti-Influenza Virus Potential of Probiotic Strain <i>Lactoplantibacillus plantarum</i> YML015 Isolated from Korean Fermented Vegetable |
| title_fullStr | Anti-Influenza Virus Potential of Probiotic Strain <i>Lactoplantibacillus plantarum</i> YML015 Isolated from Korean Fermented Vegetable |
| title_full_unstemmed | Anti-Influenza Virus Potential of Probiotic Strain <i>Lactoplantibacillus plantarum</i> YML015 Isolated from Korean Fermented Vegetable |
| title_short | Anti-Influenza Virus Potential of Probiotic Strain <i>Lactoplantibacillus plantarum</i> YML015 Isolated from Korean Fermented Vegetable |
| title_sort | anti influenza virus potential of probiotic strain i lactoplantibacillus plantarum i yml015 isolated from korean fermented vegetable |
| topic | probiotic <i>L. plantarum</i> YML015 antiviral activity |
| url | https://www.mdpi.com/2311-5637/8/11/572 |
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