Warburg-associated acidification represses lactic fermentation independently of lactate, contribution from real-time NMR on cell-free systems
Abstract Lactate accumulation and acidification in tumours are a cancer hallmark associated with the Warburg effect. Lactic acidosis correlates with cancer malignancy, and the benefit it offers to tumours has been the subject of numerous hypotheses. Strikingly, lactic acidosis enhances cancer cell s...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-44783-3 |
| _version_ | 1797559629702496256 |
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| author | Zoé Daverio Maxime Kolkman Johan Perrier Lexane Brunet Nadia Bendridi Corinne Sanglar Marie-Agnès Berger Baptiste Panthu Gilles J. P. Rautureau |
| author_facet | Zoé Daverio Maxime Kolkman Johan Perrier Lexane Brunet Nadia Bendridi Corinne Sanglar Marie-Agnès Berger Baptiste Panthu Gilles J. P. Rautureau |
| author_sort | Zoé Daverio |
| collection | DOAJ |
| description | Abstract Lactate accumulation and acidification in tumours are a cancer hallmark associated with the Warburg effect. Lactic acidosis correlates with cancer malignancy, and the benefit it offers to tumours has been the subject of numerous hypotheses. Strikingly, lactic acidosis enhances cancer cell survival to environmental glucose depletion by repressing high-rate glycolysis and lactic fermentation, and promoting an oxidative metabolism involving reactivated respiration. We used real-time NMR to evaluate how cytosolic lactate accumulation up to 40 mM and acidification up to pH 6.5 individually impact glucose consumption, lactate production and pyruvate evolution in isolated cytosols. We used a reductive cell-free system (CFS) to specifically study cytosolic metabolism independently of other Warburg-regulatory mechanisms found in the cell. We assessed the impact of lactate and acidification on the Warburg metabolism of cancer cytosols, and whether this effect extended to different cytosolic phenotypes of lactic fermentation and cancer. We observed that moderate acidification, independently of lactate concentration, drastically reduces the glucose consumption rate and halts lactate production in different lactic fermentation phenotypes. In parallel, for Warburg-type CFS lactate supplementation induces pyruvate accumulation at control pH, and can maintain a higher cytosolic pyruvate pool at low pH. Altogether, we demonstrate that intracellular acidification accounts for the direct repression of lactic fermentation by the Warburg-associated lactic acidosis. |
| first_indexed | 2024-03-10T17:48:02Z |
| format | Article |
| id | doaj.art-8cd5baad18be4c728dc663eda9f56d24 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-10T17:48:02Z |
| publishDate | 2023-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-8cd5baad18be4c728dc663eda9f56d242023-11-20T09:27:32ZengNature PortfolioScientific Reports2045-23222023-10-0113111110.1038/s41598-023-44783-3Warburg-associated acidification represses lactic fermentation independently of lactate, contribution from real-time NMR on cell-free systemsZoé Daverio0Maxime Kolkman1Johan Perrier2Lexane Brunet3Nadia Bendridi4Corinne Sanglar5Marie-Agnès Berger6Baptiste Panthu7Gilles J. P. Rautureau8Laboratoire CarMeN, UMR INSERM U1060/INRAE U1397, University of Lyon, Université Claude Bernard Lyon 1Laboratoire CarMeN, UMR INSERM U1060/INRAE U1397, University of Lyon, Université Claude Bernard Lyon 1Laboratoire CarMeN, UMR INSERM U1060/INRAE U1397, University of Lyon, Université Claude Bernard Lyon 1Laboratoire CarMeN, UMR INSERM U1060/INRAE U1397, University of Lyon, Université Claude Bernard Lyon 1Laboratoire CarMeN, UMR INSERM U1060/INRAE U1397, University of Lyon, Université Claude Bernard Lyon 1Institut des Sciences Analytiques, UMR5280 CNRS, University of Lyon, Université Claude Bernard Lyon 1Laboratoire CarMeN, UMR INSERM U1060/INRAE U1397, University of Lyon, Université Claude Bernard Lyon 1Laboratoire CarMeN, UMR INSERM U1060/INRAE U1397, University of Lyon, Université Claude Bernard Lyon 1Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, ICBMS UMR 5246, University of Lyon, Université Claude Bernard Lyon 1Abstract Lactate accumulation and acidification in tumours are a cancer hallmark associated with the Warburg effect. Lactic acidosis correlates with cancer malignancy, and the benefit it offers to tumours has been the subject of numerous hypotheses. Strikingly, lactic acidosis enhances cancer cell survival to environmental glucose depletion by repressing high-rate glycolysis and lactic fermentation, and promoting an oxidative metabolism involving reactivated respiration. We used real-time NMR to evaluate how cytosolic lactate accumulation up to 40 mM and acidification up to pH 6.5 individually impact glucose consumption, lactate production and pyruvate evolution in isolated cytosols. We used a reductive cell-free system (CFS) to specifically study cytosolic metabolism independently of other Warburg-regulatory mechanisms found in the cell. We assessed the impact of lactate and acidification on the Warburg metabolism of cancer cytosols, and whether this effect extended to different cytosolic phenotypes of lactic fermentation and cancer. We observed that moderate acidification, independently of lactate concentration, drastically reduces the glucose consumption rate and halts lactate production in different lactic fermentation phenotypes. In parallel, for Warburg-type CFS lactate supplementation induces pyruvate accumulation at control pH, and can maintain a higher cytosolic pyruvate pool at low pH. Altogether, we demonstrate that intracellular acidification accounts for the direct repression of lactic fermentation by the Warburg-associated lactic acidosis.https://doi.org/10.1038/s41598-023-44783-3 |
| spellingShingle | Zoé Daverio Maxime Kolkman Johan Perrier Lexane Brunet Nadia Bendridi Corinne Sanglar Marie-Agnès Berger Baptiste Panthu Gilles J. P. Rautureau Warburg-associated acidification represses lactic fermentation independently of lactate, contribution from real-time NMR on cell-free systems Scientific Reports |
| title | Warburg-associated acidification represses lactic fermentation independently of lactate, contribution from real-time NMR on cell-free systems |
| title_full | Warburg-associated acidification represses lactic fermentation independently of lactate, contribution from real-time NMR on cell-free systems |
| title_fullStr | Warburg-associated acidification represses lactic fermentation independently of lactate, contribution from real-time NMR on cell-free systems |
| title_full_unstemmed | Warburg-associated acidification represses lactic fermentation independently of lactate, contribution from real-time NMR on cell-free systems |
| title_short | Warburg-associated acidification represses lactic fermentation independently of lactate, contribution from real-time NMR on cell-free systems |
| title_sort | warburg associated acidification represses lactic fermentation independently of lactate contribution from real time nmr on cell free systems |
| url | https://doi.org/10.1038/s41598-023-44783-3 |
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