Gab2 Promotes Invasion and Metastasis of Gastric Cancer via Regulating EMT
Objective To investigate the expression of Grb2 binding protein-2(Gab2) in gastric cancers and its significance and mechanism in the invasiveness and metastasis of gastric cancer. Methods Immunohistochemical method was used to detect the expression of Gab2, E-cadherin, N-cadherin and MMP-9 in gastri...
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| Format: | Article |
| Language: | zho |
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Magazine House of Cancer Research on Prevention and Treatment
2018-06-01
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| Series: | Zhongliu Fangzhi Yanjiu |
| Subjects: | |
| Online Access: | http://html.rhhz.net/ZLFZYJ/html/8578.2018.17.0881.htm |
| _version_ | 1819168790824353792 |
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| author | CHEN Meiling JIANG Yingxiao WANG Shuxiao SUN Xiuning ZHANG Xiaoqian ZHENG Shuxian ZHANG Baogang SHI Lihong |
| author_facet | CHEN Meiling JIANG Yingxiao WANG Shuxiao SUN Xiuning ZHANG Xiaoqian ZHENG Shuxian ZHANG Baogang SHI Lihong |
| author_sort | CHEN Meiling |
| collection | DOAJ |
| description | Objective To investigate the expression of Grb2 binding protein-2(Gab2) in gastric cancers and its significance and mechanism in the invasiveness and metastasis of gastric cancer. Methods Immunohistochemical method was used to detect the expression of Gab2, E-cadherin, N-cadherin and MMP-9 in gastric cancer tissues. The relationship between Gab2 expression and lymphatic metastasis was analysed. SiRNA plasmid targeting Gab2 and empty vector plasmid were transfected into SGC7901 cells and named siGab2/SGC7901 and NC/SGC7901 cells respectively. RT-PCR method was applied to analyze mRNA expression of Gab2 in siGab2/SGC7901 and NC/SGC7901 cells. In vitro matrigel invasion assay was used to detect the invasiveness of siGab2/SGC7901 and SGC7901 cells. Western blot was applied to analyze protein expression. Results Gab2 expression in gastric cancer tissues was related with lymphatic metastasis significantly(P=0.002), E-cadherin expression level negatively (r=-0.693, P=0.000), and N-cadherin and MMP-9 levels positively (r=0.407, P=0.021; r=0.335, P=0.017). After microRNA interference, the mRNA and protein expressions of Gab2 in siGab2/SGC7901 cells were decreased obviously, moreover, the quantity of gastric cancer cells which invaded and penetrated matrigel membrane was decreased. Western blot showed that the expressions of N-cadherin and MMP-9 were decreased and E-cadherin expression was increased in siGab2/SGC7901 cells. Conclusion Gab2 plays a key role in the invasiveness and metastasis of gastric cancer by regulating EMT. |
| first_indexed | 2024-12-22T19:09:12Z |
| format | Article |
| id | doaj.art-8cd6085fd9354c9f9aa7f17c06b10b91 |
| institution | Directory Open Access Journal |
| issn | 1000-8578 1000-8578 |
| language | zho |
| last_indexed | 2024-12-22T19:09:12Z |
| publishDate | 2018-06-01 |
| publisher | Magazine House of Cancer Research on Prevention and Treatment |
| record_format | Article |
| series | Zhongliu Fangzhi Yanjiu |
| spelling | doaj.art-8cd6085fd9354c9f9aa7f17c06b10b912022-12-21T18:15:43ZzhoMagazine House of Cancer Research on Prevention and TreatmentZhongliu Fangzhi Yanjiu1000-85781000-85782018-06-0145638138510.3971/j.issn.1000-8578.2018.17.08818578.2018.17.0881Gab2 Promotes Invasion and Metastasis of Gastric Cancer via Regulating EMTCHEN Meiling0JIANG Yingxiao1WANG Shuxiao2SUN Xiuning3ZHANG Xiaoqian4ZHENG Shuxian5ZHANG Baogang6SHI Lihong7Shandong Province Key Laboratory of Applied Pharmacology, Weifang Medical University, Weifang 261053, ChinaDepartment of Radiotherapy, Weifang People's Hospital, Weifang 261041, ChinaShandong Province Key Laboratory of Applied Pharmacology, Weifang Medical University, Weifang 261053, ChinaDepartment of Pathogeny Microbiology, Weifang Medical University, Weifang 261053, ChinaDepartment of Gastroenterology, Affiliated Hospital of Weifang Medical University, Weifang 261042, ChinaDepartment of Pathology, Weifang Medical University, Weifang 261053, ChinaDepartment of Pathology, Weifang Medical University, Weifang 261053, ChinaShandong Province Key Laboratory of Applied Pharmacology, Weifang Medical University, Weifang 261053, ChinaObjective To investigate the expression of Grb2 binding protein-2(Gab2) in gastric cancers and its significance and mechanism in the invasiveness and metastasis of gastric cancer. Methods Immunohistochemical method was used to detect the expression of Gab2, E-cadherin, N-cadherin and MMP-9 in gastric cancer tissues. The relationship between Gab2 expression and lymphatic metastasis was analysed. SiRNA plasmid targeting Gab2 and empty vector plasmid were transfected into SGC7901 cells and named siGab2/SGC7901 and NC/SGC7901 cells respectively. RT-PCR method was applied to analyze mRNA expression of Gab2 in siGab2/SGC7901 and NC/SGC7901 cells. In vitro matrigel invasion assay was used to detect the invasiveness of siGab2/SGC7901 and SGC7901 cells. Western blot was applied to analyze protein expression. Results Gab2 expression in gastric cancer tissues was related with lymphatic metastasis significantly(P=0.002), E-cadherin expression level negatively (r=-0.693, P=0.000), and N-cadherin and MMP-9 levels positively (r=0.407, P=0.021; r=0.335, P=0.017). After microRNA interference, the mRNA and protein expressions of Gab2 in siGab2/SGC7901 cells were decreased obviously, moreover, the quantity of gastric cancer cells which invaded and penetrated matrigel membrane was decreased. Western blot showed that the expressions of N-cadherin and MMP-9 were decreased and E-cadherin expression was increased in siGab2/SGC7901 cells. Conclusion Gab2 plays a key role in the invasiveness and metastasis of gastric cancer by regulating EMT.http://html.rhhz.net/ZLFZYJ/html/8578.2018.17.0881.htmgastric cancersgc7901gab2emtmmp-9invasiveness |
| spellingShingle | CHEN Meiling JIANG Yingxiao WANG Shuxiao SUN Xiuning ZHANG Xiaoqian ZHENG Shuxian ZHANG Baogang SHI Lihong Gab2 Promotes Invasion and Metastasis of Gastric Cancer via Regulating EMT Zhongliu Fangzhi Yanjiu gastric cancer sgc7901 gab2 emt mmp-9 invasiveness |
| title | Gab2 Promotes Invasion and Metastasis of Gastric Cancer via Regulating EMT |
| title_full | Gab2 Promotes Invasion and Metastasis of Gastric Cancer via Regulating EMT |
| title_fullStr | Gab2 Promotes Invasion and Metastasis of Gastric Cancer via Regulating EMT |
| title_full_unstemmed | Gab2 Promotes Invasion and Metastasis of Gastric Cancer via Regulating EMT |
| title_short | Gab2 Promotes Invasion and Metastasis of Gastric Cancer via Regulating EMT |
| title_sort | gab2 promotes invasion and metastasis of gastric cancer via regulating emt |
| topic | gastric cancer sgc7901 gab2 emt mmp-9 invasiveness |
| url | http://html.rhhz.net/ZLFZYJ/html/8578.2018.17.0881.htm |
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