Design of biomimetic cellular scaffolds for co-culture system and their application
The extracellular matrix of most natural tissues comprises various types of cells, including fibroblasts, stem cells, and endothelial cells, which communicate with each other directly or indirectly to regulate matrix production and cell functionality. To engineer multicellular interactions in vitro,...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2017-08-01
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| Series: | Journal of Tissue Engineering |
| Online Access: | https://doi.org/10.1177/2041731417724640 |
| _version_ | 1818146574344126464 |
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| author | Yun-Min Kook Yoon Jeong Kangwon Lee Won-Gun Koh |
| author_facet | Yun-Min Kook Yoon Jeong Kangwon Lee Won-Gun Koh |
| author_sort | Yun-Min Kook |
| collection | DOAJ |
| description | The extracellular matrix of most natural tissues comprises various types of cells, including fibroblasts, stem cells, and endothelial cells, which communicate with each other directly or indirectly to regulate matrix production and cell functionality. To engineer multicellular interactions in vitro, co-culture systems have achieved tremendous success achieving a more realistic microenvironment of in vivo metabolism than monoculture system in the past several decades. Recently, the fields of tissue engineering and regenerative medicine have primarily focused on three-dimensional co-culture systems using cellular scaffolds, because of their physical and biological relevance to the extracellular matrix of actual tissues. This review discusses several materials and methods to create co-culture systems, including hydrogels, electrospun fibers, microfluidic devices, and patterning for biomimetic co-culture system and their applications for specific tissue regeneration. Consequently, we believe that culture systems with appropriate physical and biochemical properties should be developed, and direct or indirect cell–cell interactions in the remodeled tissue must be considered to obtain an optimal tissue-specific microenvironment. |
| first_indexed | 2024-12-11T12:21:31Z |
| format | Article |
| id | doaj.art-8cd6381b4d754d0a9bf116732288e2cd |
| institution | Directory Open Access Journal |
| issn | 2041-7314 |
| language | English |
| last_indexed | 2024-12-11T12:21:31Z |
| publishDate | 2017-08-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Tissue Engineering |
| spelling | doaj.art-8cd6381b4d754d0a9bf116732288e2cd2022-12-22T01:07:31ZengSAGE PublishingJournal of Tissue Engineering2041-73142017-08-01810.1177/2041731417724640Design of biomimetic cellular scaffolds for co-culture system and their applicationYun-Min Kook0Yoon Jeong1Kangwon Lee2Won-Gun Koh3Department of Chemical and Biomolecular Engineering, Yonsei University, Seoul, Republic of KoreaProgram in Nanoscience and Technology, Graduate School of Convergence Science and Technology, Seoul National University, Seoul, KoreaAdvanced Institutes of Convergence Technology, Suwon, KoreaDepartment of Chemical and Biomolecular Engineering, Yonsei University, Seoul, Republic of KoreaThe extracellular matrix of most natural tissues comprises various types of cells, including fibroblasts, stem cells, and endothelial cells, which communicate with each other directly or indirectly to regulate matrix production and cell functionality. To engineer multicellular interactions in vitro, co-culture systems have achieved tremendous success achieving a more realistic microenvironment of in vivo metabolism than monoculture system in the past several decades. Recently, the fields of tissue engineering and regenerative medicine have primarily focused on three-dimensional co-culture systems using cellular scaffolds, because of their physical and biological relevance to the extracellular matrix of actual tissues. This review discusses several materials and methods to create co-culture systems, including hydrogels, electrospun fibers, microfluidic devices, and patterning for biomimetic co-culture system and their applications for specific tissue regeneration. Consequently, we believe that culture systems with appropriate physical and biochemical properties should be developed, and direct or indirect cell–cell interactions in the remodeled tissue must be considered to obtain an optimal tissue-specific microenvironment.https://doi.org/10.1177/2041731417724640 |
| spellingShingle | Yun-Min Kook Yoon Jeong Kangwon Lee Won-Gun Koh Design of biomimetic cellular scaffolds for co-culture system and their application Journal of Tissue Engineering |
| title | Design of biomimetic cellular scaffolds for co-culture system and their application |
| title_full | Design of biomimetic cellular scaffolds for co-culture system and their application |
| title_fullStr | Design of biomimetic cellular scaffolds for co-culture system and their application |
| title_full_unstemmed | Design of biomimetic cellular scaffolds for co-culture system and their application |
| title_short | Design of biomimetic cellular scaffolds for co-culture system and their application |
| title_sort | design of biomimetic cellular scaffolds for co culture system and their application |
| url | https://doi.org/10.1177/2041731417724640 |
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