Seed germination and antioxidant enzyme activity in seedlings of diploid and tetraploid bahiagrass under water restriction
ABSTRACT: The objective of the following research was to investigate the physiological responses of two bahiagrass genotypes (Paspalum notatum Flügge) submitted to water restriction, induced by polyethylene glycol (PEG6000), during seed germination. Seeds of cv. Pensacola (diploid) and Bagual ecotyp...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Santa Maria
2020-04-01
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| Series: | Ciência Rural |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000500202&tlng=en |
| _version_ | 1828131491869097984 |
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| author | Gabriel Streck Bortolin Alfredo José Salinas-Arcienega Yutcelia Carolina Galviz-Fajardo Luciano do Amarante Carlos Eduardo da Silva Pedroso João Carlos Pinto Oliveira Mauricio Marini Köpp Andrea Bicca Noguez Martins Lilian Vanussa Madruga de Tunes |
| author_facet | Gabriel Streck Bortolin Alfredo José Salinas-Arcienega Yutcelia Carolina Galviz-Fajardo Luciano do Amarante Carlos Eduardo da Silva Pedroso João Carlos Pinto Oliveira Mauricio Marini Köpp Andrea Bicca Noguez Martins Lilian Vanussa Madruga de Tunes |
| author_sort | Gabriel Streck Bortolin |
| collection | DOAJ |
| description | ABSTRACT: The objective of the following research was to investigate the physiological responses of two bahiagrass genotypes (Paspalum notatum Flügge) submitted to water restriction, induced by polyethylene glycol (PEG6000), during seed germination. Seeds of cv. Pensacola (diploid) and Bagual ecotype (tetraploid) were placed on paper moistened with osmotic solutions at potentials of 0.0; -0.1; -0.2 and -0.3 MPa. Composed of two subsamples of 100 seeds and four experimental units per treatment, the germination test was conducted in a germination chamber under alternating temperatures of 30 °C (8 hours) and 20 °C (16 hours), with presence of light during the higher temperature. Final germination percentage and germination speed index of the seeds, as well as early growth, activity and expression of antioxidant enzymes of seedlings were evaluated. The water restriction induced by PEG6000 reduced the percentage of germination in Pensacola (from 74% to 59%) and Bagual (34% to 10%). For the latter, the evaluation of seedlings showed higher dry mass in relation to cv. Pensacola (1.7 times higher in root and 1.6 times in shoot). In response to moderate water restriction, the genotypes showed increases in root growth (1.7 times higher in -0.1 compared to 0.0 MPa), both in expression and enzymatic activity (superoxide dismutase and catalase). |
| first_indexed | 2024-04-11T16:49:01Z |
| format | Article |
| id | doaj.art-8cd652b26c8840d092d7ce60f89faaf0 |
| institution | Directory Open Access Journal |
| issn | 1678-4596 |
| language | English |
| last_indexed | 2024-04-11T16:49:01Z |
| publishDate | 2020-04-01 |
| publisher | Universidade Federal de Santa Maria |
| record_format | Article |
| series | Ciência Rural |
| spelling | doaj.art-8cd652b26c8840d092d7ce60f89faaf02022-12-22T04:13:30ZengUniversidade Federal de Santa MariaCiência Rural1678-45962020-04-0150510.1590/0103-8478cr20190382Seed germination and antioxidant enzyme activity in seedlings of diploid and tetraploid bahiagrass under water restrictionGabriel Streck Bortolinhttps://orcid.org/0000-0001-5552-3720Alfredo José Salinas-Arcienegahttps://orcid.org/0000-0001-9377-4803Yutcelia Carolina Galviz-Fajardohttps://orcid.org/0000-0003-3811-5877Luciano do Amarantehttps://orcid.org/0000-0002-4219-9404Carlos Eduardo da Silva Pedrosohttps://orcid.org/0000-0003-3253-6152João Carlos Pinto Oliveirahttps://orcid.org/0000-0003-2415-6651Mauricio Marini Köpphttps://orcid.org/0000-0002-3961-1955Andrea Bicca Noguez Martinshttps://orcid.org/0000-0001-5480-5208Lilian Vanussa Madruga de Tuneshttps://orcid.org/0000-0001-7562-1926ABSTRACT: The objective of the following research was to investigate the physiological responses of two bahiagrass genotypes (Paspalum notatum Flügge) submitted to water restriction, induced by polyethylene glycol (PEG6000), during seed germination. Seeds of cv. Pensacola (diploid) and Bagual ecotype (tetraploid) were placed on paper moistened with osmotic solutions at potentials of 0.0; -0.1; -0.2 and -0.3 MPa. Composed of two subsamples of 100 seeds and four experimental units per treatment, the germination test was conducted in a germination chamber under alternating temperatures of 30 °C (8 hours) and 20 °C (16 hours), with presence of light during the higher temperature. Final germination percentage and germination speed index of the seeds, as well as early growth, activity and expression of antioxidant enzymes of seedlings were evaluated. The water restriction induced by PEG6000 reduced the percentage of germination in Pensacola (from 74% to 59%) and Bagual (34% to 10%). For the latter, the evaluation of seedlings showed higher dry mass in relation to cv. Pensacola (1.7 times higher in root and 1.6 times in shoot). In response to moderate water restriction, the genotypes showed increases in root growth (1.7 times higher in -0.1 compared to 0.0 MPa), both in expression and enzymatic activity (superoxide dismutase and catalase).http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000500202&tlng=enPaspalum notatum Flüggeecotypepolyethylene glycoldrought stressoxidative stress |
| spellingShingle | Gabriel Streck Bortolin Alfredo José Salinas-Arcienega Yutcelia Carolina Galviz-Fajardo Luciano do Amarante Carlos Eduardo da Silva Pedroso João Carlos Pinto Oliveira Mauricio Marini Köpp Andrea Bicca Noguez Martins Lilian Vanussa Madruga de Tunes Seed germination and antioxidant enzyme activity in seedlings of diploid and tetraploid bahiagrass under water restriction Ciência Rural Paspalum notatum Flügge ecotype polyethylene glycol drought stress oxidative stress |
| title | Seed germination and antioxidant enzyme activity in seedlings of diploid and tetraploid bahiagrass under water restriction |
| title_full | Seed germination and antioxidant enzyme activity in seedlings of diploid and tetraploid bahiagrass under water restriction |
| title_fullStr | Seed germination and antioxidant enzyme activity in seedlings of diploid and tetraploid bahiagrass under water restriction |
| title_full_unstemmed | Seed germination and antioxidant enzyme activity in seedlings of diploid and tetraploid bahiagrass under water restriction |
| title_short | Seed germination and antioxidant enzyme activity in seedlings of diploid and tetraploid bahiagrass under water restriction |
| title_sort | seed germination and antioxidant enzyme activity in seedlings of diploid and tetraploid bahiagrass under water restriction |
| topic | Paspalum notatum Flügge ecotype polyethylene glycol drought stress oxidative stress |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000500202&tlng=en |
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