Simultaneous Determination of Fucoxanthin and Its Deacetylated Metabolite Fucoxanthinol in Rat Plasma by Liquid Chromatography-Tandem Mass Spectrometry
Fucoxanthin and its deacetylated metabolite fucoxanthinol are two major carotenoids that have been confirmed to possess various pharmacological properties. In the present study, fucoxanthinol was identified as the deacetylated metabolite of fucoxanthin, after intravenous (i.v.) and intragastric gava...
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MDPI AG
2015-10-01
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| Series: | Marine Drugs |
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| Online Access: | http://www.mdpi.com/1660-3397/13/10/6521 |
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| author | Yiping Zhang Hao Wu Hongmei Wen Hua Fang Zhuan Hong Ruizao Yi Rui Liu |
| author_facet | Yiping Zhang Hao Wu Hongmei Wen Hua Fang Zhuan Hong Ruizao Yi Rui Liu |
| author_sort | Yiping Zhang |
| collection | DOAJ |
| description | Fucoxanthin and its deacetylated metabolite fucoxanthinol are two major carotenoids that have been confirmed to possess various pharmacological properties. In the present study, fucoxanthinol was identified as the deacetylated metabolite of fucoxanthin, after intravenous (i.v.) and intragastric gavage (i.g.) administration to rats at doses of 2 and 65 mg/kg, respectively, by liquid chromatography-tandem mass spectrometric (LC-MS/MS) analysis. Next, an accurate and precise LC-MS/MS method was developed to quantitatively determine fucoxanthin and fucoxanthinol in rat plasma. Plasma samples were resolved by LC-MS/MS on a reverse-phase SB-C18 column that was equilibrated and eluted with acetonitrile (A)/aqueous 0.1% formic acid (B; 92/8, v/v) at a flow rate of 0.5 mL/min. Analytes were monitored by multiple-reaction monitoring (MRM) under positive electrospray ionization mode. The precursor/product transitions (m/z) were 659.3→109.0 for fucoxanthin, 617.2→109.0 for fucoxanthinol, and 429.4→313.2 for the internal standard (IS). Calibration curves for fucoxanthin and fucoxanthinol were linear over concentrations ranging from 1.53 to 720 and 1.17 to 600 ng/mL, respectively. The inter- and intraday accuracy and precision were within ±15%. The method was applied successfully in a pharmacokinetic study and the resulting oral fucoxanthin bioavailability calculated. |
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| last_indexed | 2024-04-13T08:04:49Z |
| publishDate | 2015-10-01 |
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| series | Marine Drugs |
| spelling | doaj.art-8cd694170d544b38ad35e16aeba21d122022-12-22T02:55:12ZengMDPI AGMarine Drugs1660-33972015-10-0113106521653610.3390/md13106521md13106521Simultaneous Determination of Fucoxanthin and Its Deacetylated Metabolite Fucoxanthinol in Rat Plasma by Liquid Chromatography-Tandem Mass SpectrometryYiping Zhang0Hao Wu1Hongmei Wen2Hua Fang3Zhuan Hong4Ruizao Yi5Rui Liu6Third Institute of Oceanophy Sgratate Oceanic Administration, Xiamen 361005, Fujian, ChinaCollege of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu, ChinaCollege of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu, ChinaThird Institute of Oceanophy Sgratate Oceanic Administration, Xiamen 361005, Fujian, ChinaThird Institute of Oceanophy Sgratate Oceanic Administration, Xiamen 361005, Fujian, ChinaThird Institute of Oceanophy Sgratate Oceanic Administration, Xiamen 361005, Fujian, ChinaCollege of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu, ChinaFucoxanthin and its deacetylated metabolite fucoxanthinol are two major carotenoids that have been confirmed to possess various pharmacological properties. In the present study, fucoxanthinol was identified as the deacetylated metabolite of fucoxanthin, after intravenous (i.v.) and intragastric gavage (i.g.) administration to rats at doses of 2 and 65 mg/kg, respectively, by liquid chromatography-tandem mass spectrometric (LC-MS/MS) analysis. Next, an accurate and precise LC-MS/MS method was developed to quantitatively determine fucoxanthin and fucoxanthinol in rat plasma. Plasma samples were resolved by LC-MS/MS on a reverse-phase SB-C18 column that was equilibrated and eluted with acetonitrile (A)/aqueous 0.1% formic acid (B; 92/8, v/v) at a flow rate of 0.5 mL/min. Analytes were monitored by multiple-reaction monitoring (MRM) under positive electrospray ionization mode. The precursor/product transitions (m/z) were 659.3→109.0 for fucoxanthin, 617.2→109.0 for fucoxanthinol, and 429.4→313.2 for the internal standard (IS). Calibration curves for fucoxanthin and fucoxanthinol were linear over concentrations ranging from 1.53 to 720 and 1.17 to 600 ng/mL, respectively. The inter- and intraday accuracy and precision were within ±15%. The method was applied successfully in a pharmacokinetic study and the resulting oral fucoxanthin bioavailability calculated.http://www.mdpi.com/1660-3397/13/10/6521fucoxanthinfucoxanthinolmetaboliteLC-MS/MSpharmacokinetics |
| spellingShingle | Yiping Zhang Hao Wu Hongmei Wen Hua Fang Zhuan Hong Ruizao Yi Rui Liu Simultaneous Determination of Fucoxanthin and Its Deacetylated Metabolite Fucoxanthinol in Rat Plasma by Liquid Chromatography-Tandem Mass Spectrometry Marine Drugs fucoxanthin fucoxanthinol metabolite LC-MS/MS pharmacokinetics |
| title | Simultaneous Determination of Fucoxanthin and Its Deacetylated Metabolite Fucoxanthinol in Rat Plasma by Liquid Chromatography-Tandem Mass Spectrometry |
| title_full | Simultaneous Determination of Fucoxanthin and Its Deacetylated Metabolite Fucoxanthinol in Rat Plasma by Liquid Chromatography-Tandem Mass Spectrometry |
| title_fullStr | Simultaneous Determination of Fucoxanthin and Its Deacetylated Metabolite Fucoxanthinol in Rat Plasma by Liquid Chromatography-Tandem Mass Spectrometry |
| title_full_unstemmed | Simultaneous Determination of Fucoxanthin and Its Deacetylated Metabolite Fucoxanthinol in Rat Plasma by Liquid Chromatography-Tandem Mass Spectrometry |
| title_short | Simultaneous Determination of Fucoxanthin and Its Deacetylated Metabolite Fucoxanthinol in Rat Plasma by Liquid Chromatography-Tandem Mass Spectrometry |
| title_sort | simultaneous determination of fucoxanthin and its deacetylated metabolite fucoxanthinol in rat plasma by liquid chromatography tandem mass spectrometry |
| topic | fucoxanthin fucoxanthinol metabolite LC-MS/MS pharmacokinetics |
| url | http://www.mdpi.com/1660-3397/13/10/6521 |
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