Valorization of a Pyrolytic Aqueous Condensate and Its Main Components for L-Malic Acid Production with <i>Aspergillus oryzae</i> DSM 1863
Pyrolytic aqueous condensate (PAC) might serve as a cost-effective substrate for microbial malic acid production, as it is an unused side stream of the fast pyrolysis of lignocellulosic biomass that contains acetol and acetate as potential carbon sources. In the present study, shake flask cultures w...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/8/3/107 |
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| author | Christin Kubisch Katrin Ochsenreither |
| author_facet | Christin Kubisch Katrin Ochsenreither |
| author_sort | Christin Kubisch |
| collection | DOAJ |
| description | Pyrolytic aqueous condensate (PAC) might serve as a cost-effective substrate for microbial malic acid production, as it is an unused side stream of the fast pyrolysis of lignocellulosic biomass that contains acetol and acetate as potential carbon sources. In the present study, shake flask cultures were performed to evaluate the suitability of acetol and its combination with acetate as substrates for growth and L-malate production with the filamentous fungus <i>Aspergillus oryzae</i>. Acetol concentrations of up to 40 g/L were shown to be utilized for fungal growth. In combination with acetate, co-metabolization of both substrates for biomass and malate formation was observed, although the maximum tolerated acetol concentration decreased to 20 g/L. Furthermore, malate production on PAC detoxified by a combination of rotary evaporation, overliming and activated carbon treatment was studied. In shake flasks, cultivation using 100% PAC resulted in the production of 3.37 ± 0.61 g/L malate, which was considerably improved by pH adjustment up to 9.77 ± 0.55 g/L. A successful scale-up to 0.5-L bioreactors was conducted, achieving comparable yields and productivities to the shake flask cultures. Accordingly, fungal malate production using PAC was successfully demonstrated, paving the way for a bio-based production of the acid. |
| first_indexed | 2024-03-09T19:52:19Z |
| format | Article |
| id | doaj.art-8cdcdb160156480490e203daf57131d2 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-09T19:52:19Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-8cdcdb160156480490e203daf57131d22023-11-24T01:08:29ZengMDPI AGFermentation2311-56372022-02-018310710.3390/fermentation8030107Valorization of a Pyrolytic Aqueous Condensate and Its Main Components for L-Malic Acid Production with <i>Aspergillus oryzae</i> DSM 1863Christin Kubisch0Katrin Ochsenreither1Institute of Process Engineering in Life Sciences 2-Technical Biology, Karlsruhe Institute of Technology (KIT), Fritz-Haber-Weg 4, 76131 Karlsruhe, GermanyInstitute of Process Engineering in Life Sciences 2-Technical Biology, Karlsruhe Institute of Technology (KIT), Fritz-Haber-Weg 4, 76131 Karlsruhe, GermanyPyrolytic aqueous condensate (PAC) might serve as a cost-effective substrate for microbial malic acid production, as it is an unused side stream of the fast pyrolysis of lignocellulosic biomass that contains acetol and acetate as potential carbon sources. In the present study, shake flask cultures were performed to evaluate the suitability of acetol and its combination with acetate as substrates for growth and L-malate production with the filamentous fungus <i>Aspergillus oryzae</i>. Acetol concentrations of up to 40 g/L were shown to be utilized for fungal growth. In combination with acetate, co-metabolization of both substrates for biomass and malate formation was observed, although the maximum tolerated acetol concentration decreased to 20 g/L. Furthermore, malate production on PAC detoxified by a combination of rotary evaporation, overliming and activated carbon treatment was studied. In shake flasks, cultivation using 100% PAC resulted in the production of 3.37 ± 0.61 g/L malate, which was considerably improved by pH adjustment up to 9.77 ± 0.55 g/L. A successful scale-up to 0.5-L bioreactors was conducted, achieving comparable yields and productivities to the shake flask cultures. Accordingly, fungal malate production using PAC was successfully demonstrated, paving the way for a bio-based production of the acid.https://www.mdpi.com/2311-5637/8/3/107pyrolysis condensateacetol utilizationacetateco-metabolizationmalate productionfungal biotechnology |
| spellingShingle | Christin Kubisch Katrin Ochsenreither Valorization of a Pyrolytic Aqueous Condensate and Its Main Components for L-Malic Acid Production with <i>Aspergillus oryzae</i> DSM 1863 Fermentation pyrolysis condensate acetol utilization acetate co-metabolization malate production fungal biotechnology |
| title | Valorization of a Pyrolytic Aqueous Condensate and Its Main Components for L-Malic Acid Production with <i>Aspergillus oryzae</i> DSM 1863 |
| title_full | Valorization of a Pyrolytic Aqueous Condensate and Its Main Components for L-Malic Acid Production with <i>Aspergillus oryzae</i> DSM 1863 |
| title_fullStr | Valorization of a Pyrolytic Aqueous Condensate and Its Main Components for L-Malic Acid Production with <i>Aspergillus oryzae</i> DSM 1863 |
| title_full_unstemmed | Valorization of a Pyrolytic Aqueous Condensate and Its Main Components for L-Malic Acid Production with <i>Aspergillus oryzae</i> DSM 1863 |
| title_short | Valorization of a Pyrolytic Aqueous Condensate and Its Main Components for L-Malic Acid Production with <i>Aspergillus oryzae</i> DSM 1863 |
| title_sort | valorization of a pyrolytic aqueous condensate and its main components for l malic acid production with i aspergillus oryzae i dsm 1863 |
| topic | pyrolysis condensate acetol utilization acetate co-metabolization malate production fungal biotechnology |
| url | https://www.mdpi.com/2311-5637/8/3/107 |
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