The Compound 2-Hexyl, 5-Propyl Resorcinol Has a Key Role in Biofilm Formation by the Biocontrol Rhizobacterium Pseudomonas chlororaphis PCL1606
The production of the compound 2-hexyl-5-propyl resorcinol (HPR) by the biocontrol rhizobacterium Pseudomonas chlororaphis PCL1606 (PcPCL1606) is crucial for fungal antagonism and biocontrol activity that protects plants against the phytopathogenic fungus Rosellinia necatrix. The production of HPR i...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.00396/full |
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| author | Claudia E. Calderón Claudia E. Calderón Sandra Tienda Sandra Tienda Zaira Heredia-Ponce Zaira Heredia-Ponce Eva Arrebola Eva Arrebola Gerardo Cárcamo-Oyarce Leo Eberl Francisco M. Cazorla Francisco M. Cazorla |
| author_facet | Claudia E. Calderón Claudia E. Calderón Sandra Tienda Sandra Tienda Zaira Heredia-Ponce Zaira Heredia-Ponce Eva Arrebola Eva Arrebola Gerardo Cárcamo-Oyarce Leo Eberl Francisco M. Cazorla Francisco M. Cazorla |
| author_sort | Claudia E. Calderón |
| collection | DOAJ |
| description | The production of the compound 2-hexyl-5-propyl resorcinol (HPR) by the biocontrol rhizobacterium Pseudomonas chlororaphis PCL1606 (PcPCL1606) is crucial for fungal antagonism and biocontrol activity that protects plants against the phytopathogenic fungus Rosellinia necatrix. The production of HPR is also involved in avocado root colonization during the biocontrol process. This pleiotrophic response prompted us to study the potential role of HPR production in biofilm formation. The swimming motility of PcPLL1606 is enhanced by the disruption of HPR production. Mutants impaired in HPR production, revealed that adhesion, colony morphology, and typical air–liquid interphase pellicles were all dependent on HPR production. The role of HPR production in biofilm architecture was also analyzed in flow chamber experiments. These experiments revealed that the HPR mutant cells had less tight unions than those producing HPR, suggesting an involvement of HPR in the production of the biofilm matrix. |
| first_indexed | 2024-04-14T01:26:22Z |
| format | Article |
| id | doaj.art-8cde41bcdbfd4871afd743f7e9c10fa9 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-14T01:26:22Z |
| publishDate | 2019-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-8cde41bcdbfd4871afd743f7e9c10fa92022-12-22T02:20:25ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-02-011010.3389/fmicb.2019.00396433674The Compound 2-Hexyl, 5-Propyl Resorcinol Has a Key Role in Biofilm Formation by the Biocontrol Rhizobacterium Pseudomonas chlororaphis PCL1606Claudia E. Calderón0Claudia E. Calderón1Sandra Tienda2Sandra Tienda3Zaira Heredia-Ponce4Zaira Heredia-Ponce5Eva Arrebola6Eva Arrebola7Gerardo Cárcamo-Oyarce8Leo Eberl9Francisco M. Cazorla10Francisco M. Cazorla11Departamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, Málaga, SpainInstituto de Hortofruticultura Subtropical y Mediterránea “La Mayora,” Consejo Superior de Investigaciones Científicas, Universidad de Málaga, IHSM-UMA-CSIC, Málaga, SpainDepartamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, Málaga, SpainInstituto de Hortofruticultura Subtropical y Mediterránea “La Mayora,” Consejo Superior de Investigaciones Científicas, Universidad de Málaga, IHSM-UMA-CSIC, Málaga, SpainDepartamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, Málaga, SpainInstituto de Hortofruticultura Subtropical y Mediterránea “La Mayora,” Consejo Superior de Investigaciones Científicas, Universidad de Málaga, IHSM-UMA-CSIC, Málaga, SpainDepartamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, Málaga, SpainInstituto de Hortofruticultura Subtropical y Mediterránea “La Mayora,” Consejo Superior de Investigaciones Científicas, Universidad de Málaga, IHSM-UMA-CSIC, Málaga, SpainDepartment of Plant and Microbial Biology, University of Zürich, Zurich, SwitzerlandDepartment of Plant and Microbial Biology, University of Zürich, Zurich, SwitzerlandDepartamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, Málaga, SpainInstituto de Hortofruticultura Subtropical y Mediterránea “La Mayora,” Consejo Superior de Investigaciones Científicas, Universidad de Málaga, IHSM-UMA-CSIC, Málaga, SpainThe production of the compound 2-hexyl-5-propyl resorcinol (HPR) by the biocontrol rhizobacterium Pseudomonas chlororaphis PCL1606 (PcPCL1606) is crucial for fungal antagonism and biocontrol activity that protects plants against the phytopathogenic fungus Rosellinia necatrix. The production of HPR is also involved in avocado root colonization during the biocontrol process. This pleiotrophic response prompted us to study the potential role of HPR production in biofilm formation. The swimming motility of PcPLL1606 is enhanced by the disruption of HPR production. Mutants impaired in HPR production, revealed that adhesion, colony morphology, and typical air–liquid interphase pellicles were all dependent on HPR production. The role of HPR production in biofilm architecture was also analyzed in flow chamber experiments. These experiments revealed that the HPR mutant cells had less tight unions than those producing HPR, suggesting an involvement of HPR in the production of the biofilm matrix.https://www.frontiersin.org/article/10.3389/fmicb.2019.00396/fullantifungalbiocontrolbiofilmmotilityadhesionconfocal laser scanning microscopy |
| spellingShingle | Claudia E. Calderón Claudia E. Calderón Sandra Tienda Sandra Tienda Zaira Heredia-Ponce Zaira Heredia-Ponce Eva Arrebola Eva Arrebola Gerardo Cárcamo-Oyarce Leo Eberl Francisco M. Cazorla Francisco M. Cazorla The Compound 2-Hexyl, 5-Propyl Resorcinol Has a Key Role in Biofilm Formation by the Biocontrol Rhizobacterium Pseudomonas chlororaphis PCL1606 Frontiers in Microbiology antifungal biocontrol biofilm motility adhesion confocal laser scanning microscopy |
| title | The Compound 2-Hexyl, 5-Propyl Resorcinol Has a Key Role in Biofilm Formation by the Biocontrol Rhizobacterium Pseudomonas chlororaphis PCL1606 |
| title_full | The Compound 2-Hexyl, 5-Propyl Resorcinol Has a Key Role in Biofilm Formation by the Biocontrol Rhizobacterium Pseudomonas chlororaphis PCL1606 |
| title_fullStr | The Compound 2-Hexyl, 5-Propyl Resorcinol Has a Key Role in Biofilm Formation by the Biocontrol Rhizobacterium Pseudomonas chlororaphis PCL1606 |
| title_full_unstemmed | The Compound 2-Hexyl, 5-Propyl Resorcinol Has a Key Role in Biofilm Formation by the Biocontrol Rhizobacterium Pseudomonas chlororaphis PCL1606 |
| title_short | The Compound 2-Hexyl, 5-Propyl Resorcinol Has a Key Role in Biofilm Formation by the Biocontrol Rhizobacterium Pseudomonas chlororaphis PCL1606 |
| title_sort | compound 2 hexyl 5 propyl resorcinol has a key role in biofilm formation by the biocontrol rhizobacterium pseudomonas chlororaphis pcl1606 |
| topic | antifungal biocontrol biofilm motility adhesion confocal laser scanning microscopy |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.00396/full |
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