Evaluation of Long Noncoding RNA-NORAD in Breast Tumor Tissues of Iranian Women
Background: Breast cancer is the leading cause of cancer death for women worldwide. Identification of novel molecular markers that are involved in tumor development has allowed cancer diagnosis, targeted therapy and monitoring the response to cancer treatment. Long non-coding RNAs (lncRNAs) are invo...
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| Format: | Article |
| Language: | fas |
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Isfahan University of Medical Sciences
2022-08-01
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| Series: | مجله دانشکده پزشکی اصفهان |
| Subjects: | |
| Online Access: | https://jims.mui.ac.ir/article_26122_dd85bebd0a217de93b544e749501a96b.pdf |
| _version_ | 1797699147131781120 |
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| author | Gazal Orak Maryam Cheraghzadeh Fatemeh Maghsoodi Fereshteh Ameli Maryam Adelipour |
| author_facet | Gazal Orak Maryam Cheraghzadeh Fatemeh Maghsoodi Fereshteh Ameli Maryam Adelipour |
| author_sort | Gazal Orak |
| collection | DOAJ |
| description | Background: Breast cancer is the leading cause of cancer death for women worldwide. Identification of novel molecular markers that are involved in tumor development has allowed cancer diagnosis, targeted therapy and monitoring the response to cancer treatment. Long non-coding RNAs (lncRNAs) are involved in the regulation of various cellular processes, including chromosome transcription and remodeling. This study aimed to investigate the expression and significance of long noncoding RNA NORAD (Non-Coding RNA Activated by DNA Damage) (lncRNA-NORAD) in breast cancer.
Methods: Breast cancer samples were obtained from Iran National Tumor bank. Total RNA was extracted from each sample and then treated with DNase. Q-PCR was used to detect the mRNA expression of lncRNA-NORAD in breast cancer and adjacent noncancerous tissues as respective controls.
Findings: Analysis of Real Time-PCR data showed that NORAD gene expression increased significantly in the breast tumor tissues compared to adjacent noncancerous tissues (P < 0.05).
Conclusion: Since the expression of lncRNA-NORAD gene is increased in breast tumor tissues compared to the normal tissue adjacent to the tumor, this gene can be considered as a suitable biomarker for breast cancer. |
| first_indexed | 2024-03-12T04:03:48Z |
| format | Article |
| id | doaj.art-8cdfb31d5acd42d39ad26dc7325459d5 |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T04:03:48Z |
| publishDate | 2022-08-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-8cdfb31d5acd42d39ad26dc7325459d52023-09-03T11:29:14ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2022-08-014067746747310.48305/jims.v40.i677.046726122Evaluation of Long Noncoding RNA-NORAD in Breast Tumor Tissues of Iranian WomenGazal Orak0Maryam Cheraghzadeh1Fatemeh Maghsoodi2Fereshteh Ameli3Maryam Adelipour4MSc Student of Clinical Biochemistry, Department of Clinical Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranAssistant Professor, Department of Clinical Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranMSc of Biostatistics, Department of Public Health, School of Health, Abadan University of Medical Sciences, Abadan, IranAssistant Professor, Department of Pathology, School of Medicine, Tehran University of Medical Sciences, Tehran, IranAssistant Professor, Department of Clinical Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranBackground: Breast cancer is the leading cause of cancer death for women worldwide. Identification of novel molecular markers that are involved in tumor development has allowed cancer diagnosis, targeted therapy and monitoring the response to cancer treatment. Long non-coding RNAs (lncRNAs) are involved in the regulation of various cellular processes, including chromosome transcription and remodeling. This study aimed to investigate the expression and significance of long noncoding RNA NORAD (Non-Coding RNA Activated by DNA Damage) (lncRNA-NORAD) in breast cancer. Methods: Breast cancer samples were obtained from Iran National Tumor bank. Total RNA was extracted from each sample and then treated with DNase. Q-PCR was used to detect the mRNA expression of lncRNA-NORAD in breast cancer and adjacent noncancerous tissues as respective controls. Findings: Analysis of Real Time-PCR data showed that NORAD gene expression increased significantly in the breast tumor tissues compared to adjacent noncancerous tissues (P < 0.05). Conclusion: Since the expression of lncRNA-NORAD gene is increased in breast tumor tissues compared to the normal tissue adjacent to the tumor, this gene can be considered as a suitable biomarker for breast cancer.https://jims.mui.ac.ir/article_26122_dd85bebd0a217de93b544e749501a96b.pdfbiomarkersbreast neoplasmslong noncoding rnagene expressionnorad |
| spellingShingle | Gazal Orak Maryam Cheraghzadeh Fatemeh Maghsoodi Fereshteh Ameli Maryam Adelipour Evaluation of Long Noncoding RNA-NORAD in Breast Tumor Tissues of Iranian Women مجله دانشکده پزشکی اصفهان biomarkers breast neoplasms long noncoding rna gene expression norad |
| title | Evaluation of Long Noncoding RNA-NORAD in Breast Tumor Tissues of Iranian Women |
| title_full | Evaluation of Long Noncoding RNA-NORAD in Breast Tumor Tissues of Iranian Women |
| title_fullStr | Evaluation of Long Noncoding RNA-NORAD in Breast Tumor Tissues of Iranian Women |
| title_full_unstemmed | Evaluation of Long Noncoding RNA-NORAD in Breast Tumor Tissues of Iranian Women |
| title_short | Evaluation of Long Noncoding RNA-NORAD in Breast Tumor Tissues of Iranian Women |
| title_sort | evaluation of long noncoding rna norad in breast tumor tissues of iranian women |
| topic | biomarkers breast neoplasms long noncoding rna gene expression norad |
| url | https://jims.mui.ac.ir/article_26122_dd85bebd0a217de93b544e749501a96b.pdf |
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