The oomycete-specific BAG subfamily maintains protein homeostasis and promotes pathogenicity in an atypical HSP70-independent manner
Summary: Protein homeostasis is vital for organisms and requires chaperones like the conserved Bcl-2-associated athanogene (BAG) co-chaperones that bind to the heat shock protein 70 (HSP70) through their C-terminal BAG domain (BD). Here, we show an unconventional BAG subfamily exclusively found in o...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124723014031 |
| _version_ | 1797448064332464128 |
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| author | Gan Ai Jierui Si Yang Cheng Rui Meng Zishan Wu Ruofei Xu Xiaodan Wang Ying Zhai Hao Peng Yurong Li Daolong Dou Maofeng Jing |
| author_facet | Gan Ai Jierui Si Yang Cheng Rui Meng Zishan Wu Ruofei Xu Xiaodan Wang Ying Zhai Hao Peng Yurong Li Daolong Dou Maofeng Jing |
| author_sort | Gan Ai |
| collection | DOAJ |
| description | Summary: Protein homeostasis is vital for organisms and requires chaperones like the conserved Bcl-2-associated athanogene (BAG) co-chaperones that bind to the heat shock protein 70 (HSP70) through their C-terminal BAG domain (BD). Here, we show an unconventional BAG subfamily exclusively found in oomycetes. Oomycete BAGs feature an atypical N-terminal BD with a short and oomycete-specific α1 helix (α1′), plus a C-terminal small heat shock protein (sHSP) domain. In oomycete pathogen Phytophthora sojae, both BD-α1′ and sHSP domains are required for P. sojae BAG (PsBAG) function in cyst germination, pathogenicity, and unfolded protein response assisting in 26S proteasome-mediated degradation of misfolded proteins. PsBAGs form homo- and heterodimers through their unique BD-α1′ to function properly, with no recruitment of HSP70s to form the common BAG-HSP70 complex found in other eukaryotes. Our study highlights an oomycete-exclusive protein homeostasis mechanism mediated by atypical BAGs, which provides a potential target for oomycete disease control. |
| first_indexed | 2024-03-09T14:04:58Z |
| format | Article |
| id | doaj.art-8ce36a4a3dc34cd0be33a69bcf5af7a1 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-03-09T14:04:58Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-8ce36a4a3dc34cd0be33a69bcf5af7a12023-11-30T05:07:02ZengElsevierCell Reports2211-12472023-11-014211113391The oomycete-specific BAG subfamily maintains protein homeostasis and promotes pathogenicity in an atypical HSP70-independent mannerGan Ai0Jierui Si1Yang Cheng2Rui Meng3Zishan Wu4Ruofei Xu5Xiaodan Wang6Ying Zhai7Hao Peng8Yurong Li9Daolong Dou10Maofeng Jing11Department of Plant Pathology, Key Laboratory of Soybean Disease and Pest Control (Ministry of Agriculture and Rural Affairs), Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Plant Pathology, Key Laboratory of Soybean Disease and Pest Control (Ministry of Agriculture and Rural Affairs), Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Plant Pathology, Key Laboratory of Soybean Disease and Pest Control (Ministry of Agriculture and Rural Affairs), Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Plant Pathology, Key Laboratory of Soybean Disease and Pest Control (Ministry of Agriculture and Rural Affairs), Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Plant Pathology, Key Laboratory of Soybean Disease and Pest Control (Ministry of Agriculture and Rural Affairs), Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Plant Pathology, Key Laboratory of Soybean Disease and Pest Control (Ministry of Agriculture and Rural Affairs), Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Plant Pathology, China Agricultural University, Beijing 100091, ChinaUSDA-ARS, Crop Diseases, Pests and Genetics Research Unit, Parlier, CA 93648, USAUSDA-ARS, Crop Diseases, Pests and Genetics Research Unit, Parlier, CA 93648, USACorteva Agriscience, Johnston, IA 50131, USADepartment of Plant Pathology, Key Laboratory of Soybean Disease and Pest Control (Ministry of Agriculture and Rural Affairs), Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Plant Pathology, Key Laboratory of Soybean Disease and Pest Control (Ministry of Agriculture and Rural Affairs), Nanjing Agricultural University, Nanjing 210095, China; Corresponding authorSummary: Protein homeostasis is vital for organisms and requires chaperones like the conserved Bcl-2-associated athanogene (BAG) co-chaperones that bind to the heat shock protein 70 (HSP70) through their C-terminal BAG domain (BD). Here, we show an unconventional BAG subfamily exclusively found in oomycetes. Oomycete BAGs feature an atypical N-terminal BD with a short and oomycete-specific α1 helix (α1′), plus a C-terminal small heat shock protein (sHSP) domain. In oomycete pathogen Phytophthora sojae, both BD-α1′ and sHSP domains are required for P. sojae BAG (PsBAG) function in cyst germination, pathogenicity, and unfolded protein response assisting in 26S proteasome-mediated degradation of misfolded proteins. PsBAGs form homo- and heterodimers through their unique BD-α1′ to function properly, with no recruitment of HSP70s to form the common BAG-HSP70 complex found in other eukaryotes. Our study highlights an oomycete-exclusive protein homeostasis mechanism mediated by atypical BAGs, which provides a potential target for oomycete disease control.http://www.sciencedirect.com/science/article/pii/S2211124723014031CP: PlantsCP: Microbiology |
| spellingShingle | Gan Ai Jierui Si Yang Cheng Rui Meng Zishan Wu Ruofei Xu Xiaodan Wang Ying Zhai Hao Peng Yurong Li Daolong Dou Maofeng Jing The oomycete-specific BAG subfamily maintains protein homeostasis and promotes pathogenicity in an atypical HSP70-independent manner Cell Reports CP: Plants CP: Microbiology |
| title | The oomycete-specific BAG subfamily maintains protein homeostasis and promotes pathogenicity in an atypical HSP70-independent manner |
| title_full | The oomycete-specific BAG subfamily maintains protein homeostasis and promotes pathogenicity in an atypical HSP70-independent manner |
| title_fullStr | The oomycete-specific BAG subfamily maintains protein homeostasis and promotes pathogenicity in an atypical HSP70-independent manner |
| title_full_unstemmed | The oomycete-specific BAG subfamily maintains protein homeostasis and promotes pathogenicity in an atypical HSP70-independent manner |
| title_short | The oomycete-specific BAG subfamily maintains protein homeostasis and promotes pathogenicity in an atypical HSP70-independent manner |
| title_sort | oomycete specific bag subfamily maintains protein homeostasis and promotes pathogenicity in an atypical hsp70 independent manner |
| topic | CP: Plants CP: Microbiology |
| url | http://www.sciencedirect.com/science/article/pii/S2211124723014031 |
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