Antibacterial effect and cytotoxic effect of supercritical extraction of thyme and thyme extract obtained through hydrodistillation process

Investigations of antibacterial effect of supercritical extraction of thyme (TNKE) and thyme extract obtained by hydrodistillation (THD) covered 18 bacteria strains from the genuses Staphylococcus, Streptococcus and Enterococcus, including the strains MRSA, the strains VRE, as well as the reference...

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Main Authors: Mišić Dušan, Nišavić Jakov, Žižović Irena, Ivanović Jasna
Format: Article
Language:English
Published: Faculty of Veterinary Medicine, Belgrade 2009-01-01
Series:Veterinarski Glasnik
Subjects:
Online Access:http://www.doiserbia.nb.rs/img/doi/0350-2457/2009/0350-24570902017M.pdf
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author Mišić Dušan
Nišavić Jakov
Žižović Irena
Ivanović Jasna
author_facet Mišić Dušan
Nišavić Jakov
Žižović Irena
Ivanović Jasna
author_sort Mišić Dušan
collection DOAJ
description Investigations of antibacterial effect of supercritical extraction of thyme (TNKE) and thyme extract obtained by hydrodistillation (THD) covered 18 bacteria strains from the genuses Staphylococcus, Streptococcus and Enterococcus, including the strains MRSA, the strains VRE, as well as the reference strains S.pyogenes ATCC 19615, S. agalactiae ATCC 27959, S. aureus ATCC 11632, and S.aureus ATCC 25923. The anibacterial effect of thyme extracts was examined using the microdillution method in bouillon, and the cytotoxic effect of thyme extract was examined on the VERO cell line. The obtained values for MIC THD extract ranged from 160μg/mL to 1280μg/mL for all bacteria strains covered by the investigations. The obtained values for MIC TNKE extract were 1280 μg/mL to 2560 μg/mL for all examined strains except for 1 strain of S. intermedius of 320 μg/mL. The examined extracts revealed a cytotoxic effect on the VERO cell line in concentrations higher than 40 μg/mL.
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spelling doaj.art-8ce628d06dc1442795c24e6abb3a42a22022-12-21T19:23:16ZengFaculty of Veterinary Medicine, BelgradeVeterinarski Glasnik0350-24572406-07712009-01-01631-2172710.2298/VETGL0902017M0350-24570902017MAntibacterial effect and cytotoxic effect of supercritical extraction of thyme and thyme extract obtained through hydrodistillation processMišić Dušan0Nišavić Jakov1Žižović Irena2Ivanović Jasna3Katedra za mikrobiologiju, Fakultet veterinarske medicine, Beograd%SR13-01.02istaKatedra za organsku hemijsku tehnologiju, Tehnološko-metalurški fakultet, Beograd%SR13-01.15.09istaInvestigations of antibacterial effect of supercritical extraction of thyme (TNKE) and thyme extract obtained by hydrodistillation (THD) covered 18 bacteria strains from the genuses Staphylococcus, Streptococcus and Enterococcus, including the strains MRSA, the strains VRE, as well as the reference strains S.pyogenes ATCC 19615, S. agalactiae ATCC 27959, S. aureus ATCC 11632, and S.aureus ATCC 25923. The anibacterial effect of thyme extracts was examined using the microdillution method in bouillon, and the cytotoxic effect of thyme extract was examined on the VERO cell line. The obtained values for MIC THD extract ranged from 160μg/mL to 1280μg/mL for all bacteria strains covered by the investigations. The obtained values for MIC TNKE extract were 1280 μg/mL to 2560 μg/mL for all examined strains except for 1 strain of S. intermedius of 320 μg/mL. The examined extracts revealed a cytotoxic effect on the VERO cell line in concentrations higher than 40 μg/mL.http://www.doiserbia.nb.rs/img/doi/0350-2457/2009/0350-24570902017M.pdfextractsthymeantibacterial effectcytotoxic effect
spellingShingle Mišić Dušan
Nišavić Jakov
Žižović Irena
Ivanović Jasna
Antibacterial effect and cytotoxic effect of supercritical extraction of thyme and thyme extract obtained through hydrodistillation process
Veterinarski Glasnik
extracts
thyme
antibacterial effect
cytotoxic effect
title Antibacterial effect and cytotoxic effect of supercritical extraction of thyme and thyme extract obtained through hydrodistillation process
title_full Antibacterial effect and cytotoxic effect of supercritical extraction of thyme and thyme extract obtained through hydrodistillation process
title_fullStr Antibacterial effect and cytotoxic effect of supercritical extraction of thyme and thyme extract obtained through hydrodistillation process
title_full_unstemmed Antibacterial effect and cytotoxic effect of supercritical extraction of thyme and thyme extract obtained through hydrodistillation process
title_short Antibacterial effect and cytotoxic effect of supercritical extraction of thyme and thyme extract obtained through hydrodistillation process
title_sort antibacterial effect and cytotoxic effect of supercritical extraction of thyme and thyme extract obtained through hydrodistillation process
topic extracts
thyme
antibacterial effect
cytotoxic effect
url http://www.doiserbia.nb.rs/img/doi/0350-2457/2009/0350-24570902017M.pdf
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AT nisavicjakov antibacterialeffectandcytotoxiceffectofsupercriticalextractionofthymeandthymeextractobtainedthroughhydrodistillationprocess
AT zizovicirena antibacterialeffectandcytotoxiceffectofsupercriticalextractionofthymeandthymeextractobtainedthroughhydrodistillationprocess
AT ivanovicjasna antibacterialeffectandcytotoxiceffectofsupercriticalextractionofthymeandthymeextractobtainedthroughhydrodistillationprocess