Small RNA-Seq Transcriptome Profiling of Mesothelial and Mesothelioma Cell Lines Revealed microRNA Dysregulation after Exposure to Asbestos-like Fibers
Environmental exposure to fibers of respirable size has been identified as a risk for public health. Experimental evidence has revealed that a variety of fibers, including fluoro-edenite, can develop chronic respiratory diseases and elicit carcinogenic effects in humans. Fluoro-edenite (FE) is a sil...
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| Format: | Article |
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MDPI AG
2023-02-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/11/2/538 |
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| author | Veronica Filetti Claudia Lombardo Carla Loreto George Dounias Massimo Bracci Serena Matera Lucia Rapisarda Venerando Rapisarda Caterina Ledda Ermanno Vitale |
| author_facet | Veronica Filetti Claudia Lombardo Carla Loreto George Dounias Massimo Bracci Serena Matera Lucia Rapisarda Venerando Rapisarda Caterina Ledda Ermanno Vitale |
| author_sort | Veronica Filetti |
| collection | DOAJ |
| description | Environmental exposure to fibers of respirable size has been identified as a risk for public health. Experimental evidence has revealed that a variety of fibers, including fluoro-edenite, can develop chronic respiratory diseases and elicit carcinogenic effects in humans. Fluoro-edenite (FE) is a silicate mineral first found in Biancavilla (Sicily, Italy) in 1997. Environmental exposure to its fibers has been correlated with a cluster of malignant pleural mesotheliomas. This neoplasm represents a public health problem due to its long latency and to its aggression not alerted by specific symptoms. Having several biomarkers providing us with data on the health state of those exposed to FE fibers or allowing an early diagnosis on malignant pleural mesothelioma, still asymptomatic patients, would be a remarkable goal. To these purposes, we reported the miRNA transcriptome in human normal mesothelial cell line (MeT-5A) and in the human malignant mesothelioma cell line (JU77) exposed and not exposed to FE fibers. The results showed a difference in the number of deregulated miRNAs between tumor and nontumor samples both exposed and not exposed to FE fibers. As a matter of fact, the effect of exposure to FE fibers is more evident in the expression of miRNA in the tumor samples than in the nontumor samples. In the present paper, several pathways involved in the pathogenesis of malignant pleural mesothelioma have been analyzed. We especially noticed the involvement of pathways that have important functions in inflammatory processes, angiogenesis, apoptosis, and necrosis. Besides this amount of data, further studies will be designed for the selection of the most significant miRNAs to test and validate their diagnostic potential, alone or in combination with other protein biomarkers, in high-risk individuals’ liquid biopsy to have a noninvasive tool of diagnosis for this neoplasm. |
| first_indexed | 2024-03-11T09:07:06Z |
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| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-11T09:07:06Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-8ceb516c0d7d4b2fa088332859f1d8e52023-11-16T19:19:42ZengMDPI AGBiomedicines2227-90592023-02-0111253810.3390/biomedicines11020538Small RNA-Seq Transcriptome Profiling of Mesothelial and Mesothelioma Cell Lines Revealed microRNA Dysregulation after Exposure to Asbestos-like FibersVeronica Filetti0Claudia Lombardo1Carla Loreto2George Dounias3Massimo Bracci4Serena Matera5Lucia Rapisarda6Venerando Rapisarda7Caterina Ledda8Ermanno Vitale9Occupational Medicine, Department of Clinical and Experimental Medicine, University of Catania, 95123 Catania, ItalyHuman Anatomy, Department of Medical and Surgical Sciences and Advanced Technologies, University of Catania, 95123 Catania, ItalyHuman Anatomy and Histology, Department of Biomedical and Biotechnological Sciences, University of Catania, 95123 Catania, ItalyDepartment of Occupational and Environmental Health, University of West Attica, 10563 Athens, GreeceOccupational Medicine, Department of Clinical and Molecular Sciences, Polytechnic University of Marche, 60126 Ancona, ItalyOccupational Medicine, Department of Clinical and Experimental Medicine, University of Catania, 95123 Catania, ItalyOccupational Medicine, Department of Clinical and Experimental Medicine, University of Catania, 95123 Catania, ItalyOccupational Medicine, Department of Clinical and Experimental Medicine, University of Catania, 95123 Catania, ItalyOccupational Medicine, Department of Clinical and Experimental Medicine, University of Catania, 95123 Catania, ItalyOccupational Medicine, Department of Clinical and Experimental Medicine, University of Catania, 95123 Catania, ItalyEnvironmental exposure to fibers of respirable size has been identified as a risk for public health. Experimental evidence has revealed that a variety of fibers, including fluoro-edenite, can develop chronic respiratory diseases and elicit carcinogenic effects in humans. Fluoro-edenite (FE) is a silicate mineral first found in Biancavilla (Sicily, Italy) in 1997. Environmental exposure to its fibers has been correlated with a cluster of malignant pleural mesotheliomas. This neoplasm represents a public health problem due to its long latency and to its aggression not alerted by specific symptoms. Having several biomarkers providing us with data on the health state of those exposed to FE fibers or allowing an early diagnosis on malignant pleural mesothelioma, still asymptomatic patients, would be a remarkable goal. To these purposes, we reported the miRNA transcriptome in human normal mesothelial cell line (MeT-5A) and in the human malignant mesothelioma cell line (JU77) exposed and not exposed to FE fibers. The results showed a difference in the number of deregulated miRNAs between tumor and nontumor samples both exposed and not exposed to FE fibers. As a matter of fact, the effect of exposure to FE fibers is more evident in the expression of miRNA in the tumor samples than in the nontumor samples. In the present paper, several pathways involved in the pathogenesis of malignant pleural mesothelioma have been analyzed. We especially noticed the involvement of pathways that have important functions in inflammatory processes, angiogenesis, apoptosis, and necrosis. Besides this amount of data, further studies will be designed for the selection of the most significant miRNAs to test and validate their diagnostic potential, alone or in combination with other protein biomarkers, in high-risk individuals’ liquid biopsy to have a noninvasive tool of diagnosis for this neoplasm.https://www.mdpi.com/2227-9059/11/2/538malignant mesotheliomaasbestos-like fibersfluoro-edenitesmall RNA-Seq transcriptome profilingbiomarkers |
| spellingShingle | Veronica Filetti Claudia Lombardo Carla Loreto George Dounias Massimo Bracci Serena Matera Lucia Rapisarda Venerando Rapisarda Caterina Ledda Ermanno Vitale Small RNA-Seq Transcriptome Profiling of Mesothelial and Mesothelioma Cell Lines Revealed microRNA Dysregulation after Exposure to Asbestos-like Fibers Biomedicines malignant mesothelioma asbestos-like fibers fluoro-edenite small RNA-Seq transcriptome profiling biomarkers |
| title | Small RNA-Seq Transcriptome Profiling of Mesothelial and Mesothelioma Cell Lines Revealed microRNA Dysregulation after Exposure to Asbestos-like Fibers |
| title_full | Small RNA-Seq Transcriptome Profiling of Mesothelial and Mesothelioma Cell Lines Revealed microRNA Dysregulation after Exposure to Asbestos-like Fibers |
| title_fullStr | Small RNA-Seq Transcriptome Profiling of Mesothelial and Mesothelioma Cell Lines Revealed microRNA Dysregulation after Exposure to Asbestos-like Fibers |
| title_full_unstemmed | Small RNA-Seq Transcriptome Profiling of Mesothelial and Mesothelioma Cell Lines Revealed microRNA Dysregulation after Exposure to Asbestos-like Fibers |
| title_short | Small RNA-Seq Transcriptome Profiling of Mesothelial and Mesothelioma Cell Lines Revealed microRNA Dysregulation after Exposure to Asbestos-like Fibers |
| title_sort | small rna seq transcriptome profiling of mesothelial and mesothelioma cell lines revealed microrna dysregulation after exposure to asbestos like fibers |
| topic | malignant mesothelioma asbestos-like fibers fluoro-edenite small RNA-Seq transcriptome profiling biomarkers |
| url | https://www.mdpi.com/2227-9059/11/2/538 |
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