Comparison of commercial kits for apoprotein A-I and apoprotein B with standardized apoprotein A-I and B radioimmunoassays performed at the Northwest Lipid Research Center.

There has recently been a proliferation of commercial kits available for apoproteins A-I and B. Since reference procedures for apoproteins have not yet been established we have elected to compare apoprotein kit methods with highly standardized apoprotein B and A-I radioimmunoassays developed at the...

Full description

Bibliographic Details
Main Authors: J J Albers, J L Adolphson
Format: Article
Language:English
Published: Elsevier 1988-01-01
Series:Journal of Lipid Research
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520385576
_version_ 1818650659361128448
author J J Albers
J L Adolphson
author_facet J J Albers
J L Adolphson
author_sort J J Albers
collection DOAJ
description There has recently been a proliferation of commercial kits available for apoproteins A-I and B. Since reference procedures for apoproteins have not yet been established we have elected to compare apoprotein kit methods with highly standardized apoprotein B and A-I radioimmunoassays developed at the Northwest Lipid Research Center. Commercial radial immunodiffusion kits for apoproteins A-I and B were obtained from three separate companies, Calbiochem, Daiichi Pure Chemicals, and Tago, and a commercial radioimmunoassay kit for apoprotein A-I was obtained from Ventrex Laboratories. Considerable differences were observed between the commercial kit methods and the Northwest Lipid Research radioimmunoassay methods. Some of the differences between methods were related to the assigned value of the reference materials. Other differences between methods were clearly method-dependent.
first_indexed 2024-12-17T01:53:44Z
format Article
id doaj.art-8cf1148f1c154a398ddbddb9e32ed665
institution Directory Open Access Journal
issn 0022-2275
language English
last_indexed 2024-12-17T01:53:44Z
publishDate 1988-01-01
publisher Elsevier
record_format Article
series Journal of Lipid Research
spelling doaj.art-8cf1148f1c154a398ddbddb9e32ed6652022-12-21T22:08:00ZengElsevierJournal of Lipid Research0022-22751988-01-01291102108Comparison of commercial kits for apoprotein A-I and apoprotein B with standardized apoprotein A-I and B radioimmunoassays performed at the Northwest Lipid Research Center.J J Albers0J L Adolphson1Department of Medicine and Pathology, University of Washington School of Medicine, Seattle 98104.Department of Medicine and Pathology, University of Washington School of Medicine, Seattle 98104.There has recently been a proliferation of commercial kits available for apoproteins A-I and B. Since reference procedures for apoproteins have not yet been established we have elected to compare apoprotein kit methods with highly standardized apoprotein B and A-I radioimmunoassays developed at the Northwest Lipid Research Center. Commercial radial immunodiffusion kits for apoproteins A-I and B were obtained from three separate companies, Calbiochem, Daiichi Pure Chemicals, and Tago, and a commercial radioimmunoassay kit for apoprotein A-I was obtained from Ventrex Laboratories. Considerable differences were observed between the commercial kit methods and the Northwest Lipid Research radioimmunoassay methods. Some of the differences between methods were related to the assigned value of the reference materials. Other differences between methods were clearly method-dependent.http://www.sciencedirect.com/science/article/pii/S0022227520385576
spellingShingle J J Albers
J L Adolphson
Comparison of commercial kits for apoprotein A-I and apoprotein B with standardized apoprotein A-I and B radioimmunoassays performed at the Northwest Lipid Research Center.
Journal of Lipid Research
title Comparison of commercial kits for apoprotein A-I and apoprotein B with standardized apoprotein A-I and B radioimmunoassays performed at the Northwest Lipid Research Center.
title_full Comparison of commercial kits for apoprotein A-I and apoprotein B with standardized apoprotein A-I and B radioimmunoassays performed at the Northwest Lipid Research Center.
title_fullStr Comparison of commercial kits for apoprotein A-I and apoprotein B with standardized apoprotein A-I and B radioimmunoassays performed at the Northwest Lipid Research Center.
title_full_unstemmed Comparison of commercial kits for apoprotein A-I and apoprotein B with standardized apoprotein A-I and B radioimmunoassays performed at the Northwest Lipid Research Center.
title_short Comparison of commercial kits for apoprotein A-I and apoprotein B with standardized apoprotein A-I and B radioimmunoassays performed at the Northwest Lipid Research Center.
title_sort comparison of commercial kits for apoprotein a i and apoprotein b with standardized apoprotein a i and b radioimmunoassays performed at the northwest lipid research center
url http://www.sciencedirect.com/science/article/pii/S0022227520385576
work_keys_str_mv AT jjalbers comparisonofcommercialkitsforapoproteinaiandapoproteinbwithstandardizedapoproteinaiandbradioimmunoassaysperformedatthenorthwestlipidresearchcenter
AT jladolphson comparisonofcommercialkitsforapoproteinaiandapoproteinbwithstandardizedapoproteinaiandbradioimmunoassaysperformedatthenorthwestlipidresearchcenter