An endophytic Schizophyllum commune Fr. exhibits in-vitro and in-vivo antidiabetic activity in streptozotocin induced diabetic rats
Abstract The present study aimed at isolation of endophytic basidiomycetous fungi and evaluation of their in-vitro and in-vivo antidiabetic potential. Preliminary screening for in-vitro activity was carried out using α-glucosidase inhibition assay. An endophytic isolate Sch1 (isolated from Aloe vera...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2021-04-01
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| Series: | AMB Express |
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| Online Access: | https://doi.org/10.1186/s13568-021-01219-3 |
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| author | Avinash Sharma Rajvir Kaur Jasleen Kaur Saweta Garg Rajbir Bhatti Amarjeet Kaur |
| author_facet | Avinash Sharma Rajvir Kaur Jasleen Kaur Saweta Garg Rajbir Bhatti Amarjeet Kaur |
| author_sort | Avinash Sharma |
| collection | DOAJ |
| description | Abstract The present study aimed at isolation of endophytic basidiomycetous fungi and evaluation of their in-vitro and in-vivo antidiabetic potential. Preliminary screening for in-vitro activity was carried out using α-glucosidase inhibition assay. An endophytic isolate Sch1 (isolated from Aloe vera), identified to be Schizophyllum commune Fr. on molecular basis, exhibiting more than 90% α-glucosidase inhibitiory activity was selected for further studies. Detailed in-vivo investigations for antidiabetic potential of ethyl acetate extract of S. commune (Sch1), at two different doses, were carried out in streptozotocin induced diabetic Wistar rats. Treatment of diabetic rats with S. commune extract caused significant decrease in blood glucose level and increase in body weight after 14 days experimental period. It significantly restored renal parameters including creatinine, blood urea nitrogen, fractional excretion of sodium, and potassium level in diabetic rats. Improvement in lipid profile and level of antioxidant parameters viz. reduced glutathione, thiobarbituric acid reactive species, and superoxide anion generation was also observed after treatment. Liver enzymes (serum glutamic pyruvic transaminase, serum glutamic-oxaloacetic transaminases, and alkaline phosphatase) homeostasis was found to be markedly improved in diabetic rats administered with S. commune extract. The effects were more pronounced at higher concentration and comparable to acarbose which was used as positive control. Phytochemical analysis revealed the presence of phenolics and terpenoids in the ethyl acetate extract. This is the first report highlighting the therapeutic potential of an endophytic S. commune in the management of diabetes. |
| first_indexed | 2024-12-20T12:09:39Z |
| format | Article |
| id | doaj.art-8cf223a412cb426494b7b936358546b7 |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-20T12:09:39Z |
| publishDate | 2021-04-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-8cf223a412cb426494b7b936358546b72022-12-21T19:41:16ZengSpringerOpenAMB Express2191-08552021-04-0111111110.1186/s13568-021-01219-3An endophytic Schizophyllum commune Fr. exhibits in-vitro and in-vivo antidiabetic activity in streptozotocin induced diabetic ratsAvinash Sharma0Rajvir Kaur1Jasleen Kaur2Saweta Garg3Rajbir Bhatti4Amarjeet Kaur5Department of Microbiology, Guru Nanak Dev UniversityDepartment of Microbiology, Guru Nanak Dev UniversityDepartment of Microbiology, Guru Nanak Dev UniversityDepartment of Pharmaceutical Sciences, Guru Nanak Dev UniversityDepartment of Pharmaceutical Sciences, Guru Nanak Dev UniversityDepartment of Microbiology, Guru Nanak Dev UniversityAbstract The present study aimed at isolation of endophytic basidiomycetous fungi and evaluation of their in-vitro and in-vivo antidiabetic potential. Preliminary screening for in-vitro activity was carried out using α-glucosidase inhibition assay. An endophytic isolate Sch1 (isolated from Aloe vera), identified to be Schizophyllum commune Fr. on molecular basis, exhibiting more than 90% α-glucosidase inhibitiory activity was selected for further studies. Detailed in-vivo investigations for antidiabetic potential of ethyl acetate extract of S. commune (Sch1), at two different doses, were carried out in streptozotocin induced diabetic Wistar rats. Treatment of diabetic rats with S. commune extract caused significant decrease in blood glucose level and increase in body weight after 14 days experimental period. It significantly restored renal parameters including creatinine, blood urea nitrogen, fractional excretion of sodium, and potassium level in diabetic rats. Improvement in lipid profile and level of antioxidant parameters viz. reduced glutathione, thiobarbituric acid reactive species, and superoxide anion generation was also observed after treatment. Liver enzymes (serum glutamic pyruvic transaminase, serum glutamic-oxaloacetic transaminases, and alkaline phosphatase) homeostasis was found to be markedly improved in diabetic rats administered with S. commune extract. The effects were more pronounced at higher concentration and comparable to acarbose which was used as positive control. Phytochemical analysis revealed the presence of phenolics and terpenoids in the ethyl acetate extract. This is the first report highlighting the therapeutic potential of an endophytic S. commune in the management of diabetes.https://doi.org/10.1186/s13568-021-01219-3Schizophyllum communeBasidiomycetesα-Glucosidase inhibitionAntidiabetic |
| spellingShingle | Avinash Sharma Rajvir Kaur Jasleen Kaur Saweta Garg Rajbir Bhatti Amarjeet Kaur An endophytic Schizophyllum commune Fr. exhibits in-vitro and in-vivo antidiabetic activity in streptozotocin induced diabetic rats AMB Express Schizophyllum commune Basidiomycetes α-Glucosidase inhibition Antidiabetic |
| title | An endophytic Schizophyllum commune Fr. exhibits in-vitro and in-vivo antidiabetic activity in streptozotocin induced diabetic rats |
| title_full | An endophytic Schizophyllum commune Fr. exhibits in-vitro and in-vivo antidiabetic activity in streptozotocin induced diabetic rats |
| title_fullStr | An endophytic Schizophyllum commune Fr. exhibits in-vitro and in-vivo antidiabetic activity in streptozotocin induced diabetic rats |
| title_full_unstemmed | An endophytic Schizophyllum commune Fr. exhibits in-vitro and in-vivo antidiabetic activity in streptozotocin induced diabetic rats |
| title_short | An endophytic Schizophyllum commune Fr. exhibits in-vitro and in-vivo antidiabetic activity in streptozotocin induced diabetic rats |
| title_sort | endophytic schizophyllum commune fr exhibits in vitro and in vivo antidiabetic activity in streptozotocin induced diabetic rats |
| topic | Schizophyllum commune Basidiomycetes α-Glucosidase inhibition Antidiabetic |
| url | https://doi.org/10.1186/s13568-021-01219-3 |
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