Molecular characterization of Cryptosporidium in calves from rural settlements in the Northwest region of the state of São Paulo, Brazil
The study was conducted on 25 properties of the settlements São José I and Salvador, located in the municipalities of Brejo Alegre and Birigui, in the State of São Paulo, Brazil. A record of variables was elaborated and included data such as gender, breed and age of the animals. A total of 231 stool...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Londrina
2019-02-01
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| Series: | Semina: Ciências Agrárias |
| Subjects: | |
| Online Access: | http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/33245 |
| _version_ | 1811296446731780096 |
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| author | Lucas Vinicius Shigaki de Matos Luiz da Silveira Neto Bruno César Miranda Oliveira Miriam Yumi Makatu Julia Cestari Pierucci Milena Araúz Viol Jancarlo Ferreira Gomes Gilson Pereira de Oliveira Giovanni Widmer Katia Denise Saraiva Bresciani |
| author_facet | Lucas Vinicius Shigaki de Matos Luiz da Silveira Neto Bruno César Miranda Oliveira Miriam Yumi Makatu Julia Cestari Pierucci Milena Araúz Viol Jancarlo Ferreira Gomes Gilson Pereira de Oliveira Giovanni Widmer Katia Denise Saraiva Bresciani |
| author_sort | Lucas Vinicius Shigaki de Matos |
| collection | DOAJ |
| description | The study was conducted on 25 properties of the settlements São José I and Salvador, located in the municipalities of Brejo Alegre and Birigui, in the State of São Paulo, Brazil. A record of variables was elaborated and included data such as gender, breed and age of the animals. A total of 231 stool samples were collected from bovines aged one to six months, 128 being females and 103 males, 131 crossbred and 100 Holstein. Among the 231 samples, 17 (7.36%) were positive for Cryptosporidium spp. both by malachite green negative staining and by nested-PCR. Of the 17 positive samples, 14 were sequenced in agarose gel. These sequences were detected between 99% and 100% of genetic similarity for the following species. One sequence was similar to C. parvum (AB513880.1), one to C. bovis (MF074602.1), two to C. ryanae (KT922233.1), one to C. felis (KM977642.1) and nine were similar for C. andersoni reference MF350628. C. andersoni was found in animals aged 2–6 months, an age group which is different from those described by several authors. The presence of C. parvum indicates that the calves in the studied region should be considered a potential source for zoonotic transmission. For the first time to our knowledge, C. felis was identified in cattle in America. |
| first_indexed | 2024-04-13T05:48:35Z |
| format | Article |
| id | doaj.art-8cf461b1375f4c2094c391bcfd891168 |
| institution | Directory Open Access Journal |
| issn | 1676-546X 1679-0359 |
| language | English |
| last_indexed | 2024-04-13T05:48:35Z |
| publishDate | 2019-02-01 |
| publisher | Universidade Estadual de Londrina |
| record_format | Article |
| series | Semina: Ciências Agrárias |
| spelling | doaj.art-8cf461b1375f4c2094c391bcfd8911682022-12-22T02:59:52ZengUniversidade Estadual de LondrinaSemina: Ciências Agrárias1676-546X1679-03592019-02-0140149149610.5433/1679-0359.2019v40n1p49118299Molecular characterization of Cryptosporidium in calves from rural settlements in the Northwest region of the state of São Paulo, BrazilLucas Vinicius Shigaki de Matos0Luiz da Silveira Neto1Bruno César Miranda Oliveira2Miriam Yumi Makatu3Julia Cestari Pierucci4Milena Araúz Viol5Jancarlo Ferreira Gomes6Gilson Pereira de Oliveira7Giovanni Widmer8Katia Denise Saraiva Bresciani9Universidade Estadual PaulistaUniversidade Federal do TocantinsFaculdade de Medicina Veterinária de AraçatubaFaculdade de Medicina Veterinária de AraçatubaUniversidade Estadual PaulistaFaculdade de Medicina Veterinária de AraçatubaUniversidade Estadual de CampinasUniversidade Estadual PaulistaTufts CummingsFaculdade de Medicina Veterinária de AraçatubaThe study was conducted on 25 properties of the settlements São José I and Salvador, located in the municipalities of Brejo Alegre and Birigui, in the State of São Paulo, Brazil. A record of variables was elaborated and included data such as gender, breed and age of the animals. A total of 231 stool samples were collected from bovines aged one to six months, 128 being females and 103 males, 131 crossbred and 100 Holstein. Among the 231 samples, 17 (7.36%) were positive for Cryptosporidium spp. both by malachite green negative staining and by nested-PCR. Of the 17 positive samples, 14 were sequenced in agarose gel. These sequences were detected between 99% and 100% of genetic similarity for the following species. One sequence was similar to C. parvum (AB513880.1), one to C. bovis (MF074602.1), two to C. ryanae (KT922233.1), one to C. felis (KM977642.1) and nine were similar for C. andersoni reference MF350628. C. andersoni was found in animals aged 2–6 months, an age group which is different from those described by several authors. The presence of C. parvum indicates that the calves in the studied region should be considered a potential source for zoonotic transmission. For the first time to our knowledge, C. felis was identified in cattle in America.http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/33245CalvesCryptosporidiosisMolecular characterizationNested-PCR. |
| spellingShingle | Lucas Vinicius Shigaki de Matos Luiz da Silveira Neto Bruno César Miranda Oliveira Miriam Yumi Makatu Julia Cestari Pierucci Milena Araúz Viol Jancarlo Ferreira Gomes Gilson Pereira de Oliveira Giovanni Widmer Katia Denise Saraiva Bresciani Molecular characterization of Cryptosporidium in calves from rural settlements in the Northwest region of the state of São Paulo, Brazil Semina: Ciências Agrárias Calves Cryptosporidiosis Molecular characterization Nested-PCR. |
| title | Molecular characterization of Cryptosporidium in calves from rural settlements in the Northwest region of the state of São Paulo, Brazil |
| title_full | Molecular characterization of Cryptosporidium in calves from rural settlements in the Northwest region of the state of São Paulo, Brazil |
| title_fullStr | Molecular characterization of Cryptosporidium in calves from rural settlements in the Northwest region of the state of São Paulo, Brazil |
| title_full_unstemmed | Molecular characterization of Cryptosporidium in calves from rural settlements in the Northwest region of the state of São Paulo, Brazil |
| title_short | Molecular characterization of Cryptosporidium in calves from rural settlements in the Northwest region of the state of São Paulo, Brazil |
| title_sort | molecular characterization of cryptosporidium in calves from rural settlements in the northwest region of the state of sao paulo brazil |
| topic | Calves Cryptosporidiosis Molecular characterization Nested-PCR. |
| url | http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/33245 |
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