Diverse bacteria elicit distinct neutrophil responses in a physiologically relevant model of infection
Summary: An efficient neutrophil response is critical for fighting bacterial infections, which remain a significant global health concern; therefore, modulating neutrophil function could be an effective therapeutic approach. While we have a general understanding of how neutrophils respond to bacteri...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223027049 |
| _version_ | 1797389422779432960 |
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| author | Isaac M. Richardson Christopher J. Calo Eric L. Ginter Elise Niehaus Kayla A. Pacheco Laurel E. Hind |
| author_facet | Isaac M. Richardson Christopher J. Calo Eric L. Ginter Elise Niehaus Kayla A. Pacheco Laurel E. Hind |
| author_sort | Isaac M. Richardson |
| collection | DOAJ |
| description | Summary: An efficient neutrophil response is critical for fighting bacterial infections, which remain a significant global health concern; therefore, modulating neutrophil function could be an effective therapeutic approach. While we have a general understanding of how neutrophils respond to bacteria, how neutrophil function differs in response to diverse bacterial infections remains unclear. Here, we use a microfluidic infection-on-a-chip device to investigate the neutrophil response to four bacterial species: Pseudomonas aeruginosa, Salmonella enterica, Listeria monocytogenes, and Staphylococcus aureus. We find enhanced neutrophil extravasation to L. monocytogenes, a limited overall response to S. aureus, and identify IL-6 as universally important for neutrophil extravasation. Furthermore, we demonstrate a higher percentage of neutrophils generate reactive oxygen species (ROS) when combating gram-negative bacteria versus gram-positive bacteria. For all bacterial species, we found the percentage of neutrophils producing ROS increased following extravasation through an endothelium, underscoring the importance of studying neutrophil function in physiologically relevant models. |
| first_indexed | 2024-03-08T22:56:39Z |
| format | Article |
| id | doaj.art-8cf49633dd23441db61359426aa72380 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-08T22:56:39Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-8cf49633dd23441db61359426aa723802023-12-16T06:08:47ZengElsevieriScience2589-00422024-01-01271108627Diverse bacteria elicit distinct neutrophil responses in a physiologically relevant model of infectionIsaac M. Richardson0Christopher J. Calo1Eric L. Ginter2Elise Niehaus3Kayla A. Pacheco4Laurel E. Hind5Department of Chemical and Biological Engineering, University of Colorado – Boulder, Boulder, CO 80303, USADepartment of Chemical and Biological Engineering, University of Colorado – Boulder, Boulder, CO 80303, USADepartment of Chemical and Biological Engineering, University of Colorado – Boulder, Boulder, CO 80303, USADepartment of Chemical and Biological Engineering, University of Colorado – Boulder, Boulder, CO 80303, USADepartment of Chemical and Biological Engineering, University of Colorado – Boulder, Boulder, CO 80303, USADepartment of Chemical and Biological Engineering, University of Colorado – Boulder, Boulder, CO 80303, USA; Corresponding authorSummary: An efficient neutrophil response is critical for fighting bacterial infections, which remain a significant global health concern; therefore, modulating neutrophil function could be an effective therapeutic approach. While we have a general understanding of how neutrophils respond to bacteria, how neutrophil function differs in response to diverse bacterial infections remains unclear. Here, we use a microfluidic infection-on-a-chip device to investigate the neutrophil response to four bacterial species: Pseudomonas aeruginosa, Salmonella enterica, Listeria monocytogenes, and Staphylococcus aureus. We find enhanced neutrophil extravasation to L. monocytogenes, a limited overall response to S. aureus, and identify IL-6 as universally important for neutrophil extravasation. Furthermore, we demonstrate a higher percentage of neutrophils generate reactive oxygen species (ROS) when combating gram-negative bacteria versus gram-positive bacteria. For all bacterial species, we found the percentage of neutrophils producing ROS increased following extravasation through an endothelium, underscoring the importance of studying neutrophil function in physiologically relevant models.http://www.sciencedirect.com/science/article/pii/S2589004223027049ImmunologyBacteriology |
| spellingShingle | Isaac M. Richardson Christopher J. Calo Eric L. Ginter Elise Niehaus Kayla A. Pacheco Laurel E. Hind Diverse bacteria elicit distinct neutrophil responses in a physiologically relevant model of infection iScience Immunology Bacteriology |
| title | Diverse bacteria elicit distinct neutrophil responses in a physiologically relevant model of infection |
| title_full | Diverse bacteria elicit distinct neutrophil responses in a physiologically relevant model of infection |
| title_fullStr | Diverse bacteria elicit distinct neutrophil responses in a physiologically relevant model of infection |
| title_full_unstemmed | Diverse bacteria elicit distinct neutrophil responses in a physiologically relevant model of infection |
| title_short | Diverse bacteria elicit distinct neutrophil responses in a physiologically relevant model of infection |
| title_sort | diverse bacteria elicit distinct neutrophil responses in a physiologically relevant model of infection |
| topic | Immunology Bacteriology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223027049 |
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