Imaging Mass Cytometry Analysis of immune Checkpoint Inhibitor-Related Pneumonitis: A Case Report
Immune checkpoint inhibitor-related pneumonitis (CIP) is a rare but well-recognized immune-related adverse event (irAE), causes 35% of irAE related deaths. However, the mechanism of CIP remains unclear and no evidence-based treatment except for glucocorticoids is available. Herein, we report the cas...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.899971/full |
| _version_ | 1818529221577801728 |
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| author | Yuan Cheng Xiao-Ming Wang Qin Hu Kunyan Sun Xiang Zhao Meng Zhang Guangfa Wang He Wang Yan Xiong |
| author_facet | Yuan Cheng Xiao-Ming Wang Qin Hu Kunyan Sun Xiang Zhao Meng Zhang Guangfa Wang He Wang Yan Xiong |
| author_sort | Yuan Cheng |
| collection | DOAJ |
| description | Immune checkpoint inhibitor-related pneumonitis (CIP) is a rare but well-recognized immune-related adverse event (irAE), causes 35% of irAE related deaths. However, the mechanism of CIP remains unclear and no evidence-based treatment except for glucocorticoids is available. Herein, we report the case of a patient with metastatic bladder cancer who received tislelizumab and was diagnosed with CIP. The patient underwent transbronchial cryobiopsy. The patient was treated with glucocorticoid, but CIP recurred when the glucocorticoid tapering. The paraffine-embedded lung tissue was sectioned, stained with 31 heavy-metal tagged antibodies, and analyzed using imaging mass cytometry (IMC) technology. We identified multiple immune cell subsets in the lung tissue and observed the infiltration of memory T cells and the CD4+ DC subset. The data indicated the great potential of IMC technology in the identification and characterization of irAEs. Further investigation is warranted to identify the mechanism of action of CIP. |
| first_indexed | 2024-12-11T17:04:09Z |
| format | Article |
| id | doaj.art-8cf4ae3075f8468a8d048d76a41b72ea |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-11T17:04:09Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-8cf4ae3075f8468a8d048d76a41b72ea2022-12-22T00:57:44ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-07-011310.3389/fimmu.2022.899971899971Imaging Mass Cytometry Analysis of immune Checkpoint Inhibitor-Related Pneumonitis: A Case ReportYuan Cheng0Xiao-Ming Wang1Qin Hu2Kunyan Sun3Xiang Zhao4Meng Zhang5Guangfa Wang6He Wang7Yan Xiong8Department of Respiratory and Critical Care Medicine, Peking University First Hospital, Beijing, ChinaSchool of Basic Medical Sciences, Wuhan University, Wuhan, ChinaFaculty of Environment and Life, Beijing University of Technology, Beijing, ChinaDepartment of Respiratory and Critical Care Medicine, Peking University First Hospital, Beijing, ChinaDepartment of Respiratory and Critical Care Medicine, Peking University First Hospital, Beijing, ChinaDepartment of Respiratory and Critical Care Medicine, Peking University First Hospital, Beijing, ChinaDepartment of Respiratory and Critical Care Medicine, Peking University First Hospital, Beijing, ChinaDepartment of Radiology, Peking University First Hospital, Beijing, ChinaDepartment of Pathology, Peking University First Hospital, Beijing, ChinaImmune checkpoint inhibitor-related pneumonitis (CIP) is a rare but well-recognized immune-related adverse event (irAE), causes 35% of irAE related deaths. However, the mechanism of CIP remains unclear and no evidence-based treatment except for glucocorticoids is available. Herein, we report the case of a patient with metastatic bladder cancer who received tislelizumab and was diagnosed with CIP. The patient underwent transbronchial cryobiopsy. The patient was treated with glucocorticoid, but CIP recurred when the glucocorticoid tapering. The paraffine-embedded lung tissue was sectioned, stained with 31 heavy-metal tagged antibodies, and analyzed using imaging mass cytometry (IMC) technology. We identified multiple immune cell subsets in the lung tissue and observed the infiltration of memory T cells and the CD4+ DC subset. The data indicated the great potential of IMC technology in the identification and characterization of irAEs. Further investigation is warranted to identify the mechanism of action of CIP.https://www.frontiersin.org/articles/10.3389/fimmu.2022.899971/fullimaging mass cytometryimmune-related adverse eventimmune checkpoint inhibitor-related pneumonitismemory T cellsheavy-metal tagged antibodies |
| spellingShingle | Yuan Cheng Xiao-Ming Wang Qin Hu Kunyan Sun Xiang Zhao Meng Zhang Guangfa Wang He Wang Yan Xiong Imaging Mass Cytometry Analysis of immune Checkpoint Inhibitor-Related Pneumonitis: A Case Report Frontiers in Immunology imaging mass cytometry immune-related adverse event immune checkpoint inhibitor-related pneumonitis memory T cells heavy-metal tagged antibodies |
| title | Imaging Mass Cytometry Analysis of immune Checkpoint Inhibitor-Related Pneumonitis: A Case Report |
| title_full | Imaging Mass Cytometry Analysis of immune Checkpoint Inhibitor-Related Pneumonitis: A Case Report |
| title_fullStr | Imaging Mass Cytometry Analysis of immune Checkpoint Inhibitor-Related Pneumonitis: A Case Report |
| title_full_unstemmed | Imaging Mass Cytometry Analysis of immune Checkpoint Inhibitor-Related Pneumonitis: A Case Report |
| title_short | Imaging Mass Cytometry Analysis of immune Checkpoint Inhibitor-Related Pneumonitis: A Case Report |
| title_sort | imaging mass cytometry analysis of immune checkpoint inhibitor related pneumonitis a case report |
| topic | imaging mass cytometry immune-related adverse event immune checkpoint inhibitor-related pneumonitis memory T cells heavy-metal tagged antibodies |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.899971/full |
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