Necrotrophic Fungus Triggers Expression of Multiple Resistance Components in Resistant and Susceptible Wheat Cultivars
Tan spot of wheat, caused by Pyrenophora tritici-repentis (Ptr), results in a yield loss through chlorosis and necrosis of healthy leaf tissue. The major objective of this study was to compare gene expression in resistant and susceptible wheat cultivars after infection with Ptr ToxA-producing race 2...
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| Format: | Article |
| Language: | English |
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Hanrimwon Publishing Company
2021-04-01
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| Series: | The Plant Pathology Journal |
| Subjects: | |
| Online Access: | http://www.ppjonline.org/upload/pdf/PPJ-OA-06-2020-0109.pdf |
| _version_ | 1811214604560236544 |
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| author | Ethan J. Andersen Madhav P. Nepal Shaukat Ali |
| author_facet | Ethan J. Andersen Madhav P. Nepal Shaukat Ali |
| author_sort | Ethan J. Andersen |
| collection | DOAJ |
| description | Tan spot of wheat, caused by Pyrenophora tritici-repentis (Ptr), results in a yield loss through chlorosis and necrosis of healthy leaf tissue. The major objective of this study was to compare gene expression in resistant and susceptible wheat cultivars after infection with Ptr ToxA-producing race 2 and direct infiltration with Ptr ToxA proteins. Greenhouse experiments included exposure of the wheat cultivars to pathogen inoculum or direct infiltration of leaf tissue with Ptr-ToxA protein isolate. Samples from the experiments were subjected to RNA sequencing. Results showed that ToxA RNA sequences were first detected in samples collected eight hours after treatments indicating that upon Ptr contact with wheat tissue, Ptr started expressing ToxA. The resistant wheat cultivar, in response to Ptr inoculum, expressed genes associated with plant resistance responses that were not expressed in the susceptible cultivar; genes of interest included five chitinases, eight transporters, five pathogen-detecting receptors, and multiple classes of signaling factors. Resistant and susceptible wheat cultivars therefore differed in their response in the expression of genes that encode chitinases, transporters, wall-associated kinases, permeases, and wound-induced proteins, among others. Plants exposed to Ptr inoculum expressed transcription factors, kinases, receptors, and peroxidases, which are not expressed as highly in the control samples or samples infiltrated with ToxA. Several of the differentially expressed genes between cultivars were found in the Ptr resistance QTLs on chromosomes 1A, 2D, 3B, and 5A. Future studies should elucidate the specific roles these genes play in the wheat response to Ptr. |
| first_indexed | 2024-04-12T06:07:22Z |
| format | Article |
| id | doaj.art-8cf80f1cc41841e5831a01f603e212de |
| institution | Directory Open Access Journal |
| issn | 1598-2254 2093-9280 |
| language | English |
| last_indexed | 2024-04-12T06:07:22Z |
| publishDate | 2021-04-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | The Plant Pathology Journal |
| spelling | doaj.art-8cf80f1cc41841e5831a01f603e212de2022-12-22T03:44:49ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542093-92802021-04-013729911410.5423/PPJ.OA.06.2020.01092235Necrotrophic Fungus Triggers Expression of Multiple Resistance Components in Resistant and Susceptible Wheat CultivarsEthan J. Andersen0Madhav P. Nepal1Shaukat Ali2 Department of Biology, Francis Marion University, Florence, SC 29506, USA Department of Biology and Microbiology, South Dakota State University, Brookings, SD 57007, USA Department of Agronomy, Horticulture and Plant Science, South Dakota State University, Brookings, SD 57007, USATan spot of wheat, caused by Pyrenophora tritici-repentis (Ptr), results in a yield loss through chlorosis and necrosis of healthy leaf tissue. The major objective of this study was to compare gene expression in resistant and susceptible wheat cultivars after infection with Ptr ToxA-producing race 2 and direct infiltration with Ptr ToxA proteins. Greenhouse experiments included exposure of the wheat cultivars to pathogen inoculum or direct infiltration of leaf tissue with Ptr-ToxA protein isolate. Samples from the experiments were subjected to RNA sequencing. Results showed that ToxA RNA sequences were first detected in samples collected eight hours after treatments indicating that upon Ptr contact with wheat tissue, Ptr started expressing ToxA. The resistant wheat cultivar, in response to Ptr inoculum, expressed genes associated with plant resistance responses that were not expressed in the susceptible cultivar; genes of interest included five chitinases, eight transporters, five pathogen-detecting receptors, and multiple classes of signaling factors. Resistant and susceptible wheat cultivars therefore differed in their response in the expression of genes that encode chitinases, transporters, wall-associated kinases, permeases, and wound-induced proteins, among others. Plants exposed to Ptr inoculum expressed transcription factors, kinases, receptors, and peroxidases, which are not expressed as highly in the control samples or samples infiltrated with ToxA. Several of the differentially expressed genes between cultivars were found in the Ptr resistance QTLs on chromosomes 1A, 2D, 3B, and 5A. Future studies should elucidate the specific roles these genes play in the wheat response to Ptr.http://www.ppjonline.org/upload/pdf/PPJ-OA-06-2020-0109.pdfpathogen resistanceplant-pathogen interactionrna-seqtan spotwheat |
| spellingShingle | Ethan J. Andersen Madhav P. Nepal Shaukat Ali Necrotrophic Fungus Triggers Expression of Multiple Resistance Components in Resistant and Susceptible Wheat Cultivars The Plant Pathology Journal pathogen resistance plant-pathogen interaction rna-seq tan spot wheat |
| title | Necrotrophic Fungus Triggers Expression of Multiple Resistance Components in Resistant and Susceptible Wheat Cultivars |
| title_full | Necrotrophic Fungus Triggers Expression of Multiple Resistance Components in Resistant and Susceptible Wheat Cultivars |
| title_fullStr | Necrotrophic Fungus Triggers Expression of Multiple Resistance Components in Resistant and Susceptible Wheat Cultivars |
| title_full_unstemmed | Necrotrophic Fungus Triggers Expression of Multiple Resistance Components in Resistant and Susceptible Wheat Cultivars |
| title_short | Necrotrophic Fungus Triggers Expression of Multiple Resistance Components in Resistant and Susceptible Wheat Cultivars |
| title_sort | necrotrophic fungus triggers expression of multiple resistance components in resistant and susceptible wheat cultivars |
| topic | pathogen resistance plant-pathogen interaction rna-seq tan spot wheat |
| url | http://www.ppjonline.org/upload/pdf/PPJ-OA-06-2020-0109.pdf |
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