Spermicidal Constituents of Ethanolic Extract of Sacoglottis gabonensis Stem Bark
Aim: To isolate the spermicidal constituents of Sacoglottis gabonensis. Materials and methods: The ethanolic extract with partitioned fractions of Sacoglottis gabonensis stem bark were subjected to sperm immobilization assay. The most active EtOAc fraction was further purifi ed by column and Semi-Pr...
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| Format: | Article |
| Language: | English |
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Pensoft Publishers
2017-12-01
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| Series: | Folia Medica |
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| Online Access: | http://www.degruyter.com/view/j/folmed.2017.59.issue-4/folmed-2017-0047/folmed-2017-0047.xml?format=INT |
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| author | Alade Gideon O. Moody Jones O. Awotona Olanrewaju R. Adesanya Saburi A. Lai Daowan Proksch Peter |
| author_facet | Alade Gideon O. Moody Jones O. Awotona Olanrewaju R. Adesanya Saburi A. Lai Daowan Proksch Peter |
| author_sort | Alade Gideon O. |
| collection | DOAJ |
| description | Aim: To isolate the spermicidal constituents of Sacoglottis gabonensis. Materials and methods: The ethanolic extract with partitioned fractions of Sacoglottis gabonensis stem bark were subjected to sperm immobilization assay. The most active EtOAc fraction was further purifi ed by column and Semi-Preparative High Performance Liquid Chromatography to give compounds which were characterized by spectroscopic methods (UV, LC/MS, and NMR). The compound(s) was also tested for sperm immobilization activity. Results: The ethanolic extract showed 100% signifi cant (p < 0.05) sperm immobilization activity at a concentration of 30 mg/mL at 20 s compared to both negative and positive controls. The most active ethyl acetate fraction yielded methyl 3,5-dihydroxy-4-methoxybenzoate, eriodictyol and bergenin. Bergenin had 100% sperm immobilization activity at 20 mg/mL in 60 s which was signifi cant (p < 0.05) also when compared to the positive and negative control while methyl 3,5-dihydroxy- 4-methoxybenzoate, eriodictyol were not active. Conclusion: The active spermicidal constituent in Sacoglottis gabonensis stem bark extract is bergenin. However, methyl 3,5-dihydroxy-4-methoxybenzoate and eriodictyol showed no activity. This plant is known for its aphrodisiac action; hence, caution may have to be exercised in its use because of its spermicidal eff ect. |
| first_indexed | 2024-12-21T10:21:26Z |
| format | Article |
| id | doaj.art-8cfbbe40cf2f4260a1b531ac1a6d1ade |
| institution | Directory Open Access Journal |
| issn | 1314-2143 |
| language | English |
| last_indexed | 2024-12-21T10:21:26Z |
| publishDate | 2017-12-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | Folia Medica |
| spelling | doaj.art-8cfbbe40cf2f4260a1b531ac1a6d1ade2022-12-21T19:07:26ZengPensoft PublishersFolia Medica1314-21432017-12-0159443744210.1515/folmed-2017-0047folmed-2017-0047Spermicidal Constituents of Ethanolic Extract of Sacoglottis gabonensis Stem BarkAlade Gideon O.0Moody Jones O.1Awotona Olanrewaju R.2Adesanya Saburi A.3Lai Daowan4Proksch Peter5Department of Pharmacognosy & Herbal Medicine, Faculty of Pharmacy, Niger Delta University, Wilberforce Island, NigeriaDepartment of Pharmacognosy, Faculty of Pharmacy, University of Ibadan, Ibadan, NigeriaDepartment of Pharmacognosy & Herbal Medicine, Faculty of Pharmacy, Niger Delta University, Wilberforce Island, NigeriaDepartment of Pharmacognosy, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife, NigeriaDepartment of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing, PR ChinaInstitute of Pharmaceutical Biology and Biotechnology, Heinrich Heine University, Dusseldorf, GermanyAim: To isolate the spermicidal constituents of Sacoglottis gabonensis. Materials and methods: The ethanolic extract with partitioned fractions of Sacoglottis gabonensis stem bark were subjected to sperm immobilization assay. The most active EtOAc fraction was further purifi ed by column and Semi-Preparative High Performance Liquid Chromatography to give compounds which were characterized by spectroscopic methods (UV, LC/MS, and NMR). The compound(s) was also tested for sperm immobilization activity. Results: The ethanolic extract showed 100% signifi cant (p < 0.05) sperm immobilization activity at a concentration of 30 mg/mL at 20 s compared to both negative and positive controls. The most active ethyl acetate fraction yielded methyl 3,5-dihydroxy-4-methoxybenzoate, eriodictyol and bergenin. Bergenin had 100% sperm immobilization activity at 20 mg/mL in 60 s which was signifi cant (p < 0.05) also when compared to the positive and negative control while methyl 3,5-dihydroxy- 4-methoxybenzoate, eriodictyol were not active. Conclusion: The active spermicidal constituent in Sacoglottis gabonensis stem bark extract is bergenin. However, methyl 3,5-dihydroxy-4-methoxybenzoate and eriodictyol showed no activity. This plant is known for its aphrodisiac action; hence, caution may have to be exercised in its use because of its spermicidal eff ect.http://www.degruyter.com/view/j/folmed.2017.59.issue-4/folmed-2017-0047/folmed-2017-0047.xml?format=INTspermimmobilizationcompoundSacoglottis gabonensis |
| spellingShingle | Alade Gideon O. Moody Jones O. Awotona Olanrewaju R. Adesanya Saburi A. Lai Daowan Proksch Peter Spermicidal Constituents of Ethanolic Extract of Sacoglottis gabonensis Stem Bark Folia Medica sperm immobilization compound Sacoglottis gabonensis |
| title | Spermicidal Constituents of Ethanolic Extract of Sacoglottis gabonensis Stem Bark |
| title_full | Spermicidal Constituents of Ethanolic Extract of Sacoglottis gabonensis Stem Bark |
| title_fullStr | Spermicidal Constituents of Ethanolic Extract of Sacoglottis gabonensis Stem Bark |
| title_full_unstemmed | Spermicidal Constituents of Ethanolic Extract of Sacoglottis gabonensis Stem Bark |
| title_short | Spermicidal Constituents of Ethanolic Extract of Sacoglottis gabonensis Stem Bark |
| title_sort | spermicidal constituents of ethanolic extract of sacoglottis gabonensis stem bark |
| topic | sperm immobilization compound Sacoglottis gabonensis |
| url | http://www.degruyter.com/view/j/folmed.2017.59.issue-4/folmed-2017-0047/folmed-2017-0047.xml?format=INT |
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