Bioinformatic identification and analyses of the non-specific lipid transfer proteins in wheat
Non-specific lipid transfer proteins (nsLTPs/LTPs) that can transport various phospholipids across the membrane in vitro are widespread in the plant kingdom, and they play important roles in many biological processes that are closely related to plant growth and development. Recently, nsLTPs have bee...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-05-01
|
| Series: | Journal of Integrative Agriculture |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311919627760 |
| _version_ | 1818437873951571968 |
|---|---|
| author | Zheng-wu FANG Yi-qin HE Yi-ke LIU Wen-qiang JIANG Jing-han SONG Shu-ping WANG Dong-fang MA Jun-liang YIN |
| author_facet | Zheng-wu FANG Yi-qin HE Yi-ke LIU Wen-qiang JIANG Jing-han SONG Shu-ping WANG Dong-fang MA Jun-liang YIN |
| author_sort | Zheng-wu FANG |
| collection | DOAJ |
| description | Non-specific lipid transfer proteins (nsLTPs/LTPs) that can transport various phospholipids across the membrane in vitro are widespread in the plant kingdom, and they play important roles in many biological processes that are closely related to plant growth and development. Recently, nsLTPs have been shown to respond to different forms of abiotic stresses. Despite the vital roles of nsLTPs in many plants, little is known about the nsLTPs in wheat. In this study, 330 nsLTP proteins were identified in wheat and they clustered into five types (1, 2, c, d, and g) by phylogenetic analysis with the nsLTPs from maize, Arabidopsis, and rice. The wheat nsLTPs of type d included three subtypes (d1, d2, and d3) and type g included seven subtypes (g1–g7). Genetic structure and motif pattern analyses showed that members of each type had similar structural composition. Moreover, GPI-anchors were found to exist in non-g type members from wheat for the first time. Chromosome mapping revealed that all five types were unevenly and unequally distributed on 21 chromosomes. Furthermore, gene duplication events contributed to the proliferation of the nsLTP genes. Large-scale data mining of RNA-seq data covering multiple growth stages and numerous stress treatments showed that the transcript levels of some of the nsLTP genes could be strongly induced by abiotic stresses, including drought and salinity, indicating their potential roles in mediating the responses of the wheat plants to these abiotic stress conditions. These findings provide comprehensive insights into the nsLTP family members in wheat, and offer candidate nsLTP genes for further studies on their roles in stress resistance and potential for improving wheat breeding programs. |
| first_indexed | 2024-12-14T17:31:36Z |
| format | Article |
| id | doaj.art-8cfedd27b250426c81bedf1b0fb43d29 |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-14T17:31:36Z |
| publishDate | 2020-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-8cfedd27b250426c81bedf1b0fb43d292022-12-21T22:53:05ZengElsevierJournal of Integrative Agriculture2095-31192020-05-0119511701185Bioinformatic identification and analyses of the non-specific lipid transfer proteins in wheatZheng-wu FANG0Yi-qin HE1Yi-ke LIU2Wen-qiang JIANG3Jing-han SONG4Shu-ping WANG5Dong-fang MA6Jun-liang YIN7Hubei Collaborative Innovation Center for Grain Industry/Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education/Hubei Engineering Research Center for Pest Forewarning and Management/College of Agriculture, Yangtze University, Jingzhou 434025, P.R.ChinaHubei Collaborative Innovation Center for Grain Industry/Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education/Hubei Engineering Research Center for Pest Forewarning and Management/College of Agriculture, Yangtze University, Jingzhou 434025, P.R.ChinaInstitute of Food Crops, Hubei Academy of Agricultural Sciences, Wuhan 430064, P.R.ChinaHubei Collaborative Innovation Center for Grain Industry/Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education/Hubei Engineering Research Center for Pest Forewarning and Management/College of Agriculture, Yangtze University, Jingzhou 434025, P.R.ChinaHubei Collaborative Innovation Center for Grain Industry/Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education/Hubei Engineering Research Center for Pest Forewarning and Management/College of Agriculture, Yangtze University, Jingzhou 434025, P.R.ChinaHubei Collaborative Innovation Center for Grain Industry/Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education/Hubei Engineering Research Center for Pest Forewarning and Management/College of Agriculture, Yangtze University, Jingzhou 434025, P.R.ChinaHubei Collaborative Innovation Center for Grain Industry/Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education/Hubei Engineering Research Center for Pest Forewarning and Management/College of Agriculture, Yangtze University, Jingzhou 434025, P.R.China; Correspondence MA Dong-fang, Tel: +86-716-8066302; Institute of Plant Protection, Sichuan Academy of Agricultural Sciences/Key Laboratory of Integrated Pest Management of Crop in Southwest China, Ministry of Agriculture and Rural Affairs, Chengdu 610066, P.R.ChinaHubei Collaborative Innovation Center for Grain Industry/Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education/Hubei Engineering Research Center for Pest Forewarning and Management/College of Agriculture, Yangtze University, Jingzhou 434025, P.R.China; Correspondence YIN Jun-liang, Tel: +86-716-8066302Non-specific lipid transfer proteins (nsLTPs/LTPs) that can transport various phospholipids across the membrane in vitro are widespread in the plant kingdom, and they play important roles in many biological processes that are closely related to plant growth and development. Recently, nsLTPs have been shown to respond to different forms of abiotic stresses. Despite the vital roles of nsLTPs in many plants, little is known about the nsLTPs in wheat. In this study, 330 nsLTP proteins were identified in wheat and they clustered into five types (1, 2, c, d, and g) by phylogenetic analysis with the nsLTPs from maize, Arabidopsis, and rice. The wheat nsLTPs of type d included three subtypes (d1, d2, and d3) and type g included seven subtypes (g1–g7). Genetic structure and motif pattern analyses showed that members of each type had similar structural composition. Moreover, GPI-anchors were found to exist in non-g type members from wheat for the first time. Chromosome mapping revealed that all five types were unevenly and unequally distributed on 21 chromosomes. Furthermore, gene duplication events contributed to the proliferation of the nsLTP genes. Large-scale data mining of RNA-seq data covering multiple growth stages and numerous stress treatments showed that the transcript levels of some of the nsLTP genes could be strongly induced by abiotic stresses, including drought and salinity, indicating their potential roles in mediating the responses of the wheat plants to these abiotic stress conditions. These findings provide comprehensive insights into the nsLTP family members in wheat, and offer candidate nsLTP genes for further studies on their roles in stress resistance and potential for improving wheat breeding programs.http://www.sciencedirect.com/science/article/pii/S2095311919627760nsLTPabiotic stresswheat genomebioinformaticsgene family |
| spellingShingle | Zheng-wu FANG Yi-qin HE Yi-ke LIU Wen-qiang JIANG Jing-han SONG Shu-ping WANG Dong-fang MA Jun-liang YIN Bioinformatic identification and analyses of the non-specific lipid transfer proteins in wheat Journal of Integrative Agriculture nsLTP abiotic stress wheat genome bioinformatics gene family |
| title | Bioinformatic identification and analyses of the non-specific lipid transfer proteins in wheat |
| title_full | Bioinformatic identification and analyses of the non-specific lipid transfer proteins in wheat |
| title_fullStr | Bioinformatic identification and analyses of the non-specific lipid transfer proteins in wheat |
| title_full_unstemmed | Bioinformatic identification and analyses of the non-specific lipid transfer proteins in wheat |
| title_short | Bioinformatic identification and analyses of the non-specific lipid transfer proteins in wheat |
| title_sort | bioinformatic identification and analyses of the non specific lipid transfer proteins in wheat |
| topic | nsLTP abiotic stress wheat genome bioinformatics gene family |
| url | http://www.sciencedirect.com/science/article/pii/S2095311919627760 |
| work_keys_str_mv | AT zhengwufang bioinformaticidentificationandanalysesofthenonspecificlipidtransferproteinsinwheat AT yiqinhe bioinformaticidentificationandanalysesofthenonspecificlipidtransferproteinsinwheat AT yikeliu bioinformaticidentificationandanalysesofthenonspecificlipidtransferproteinsinwheat AT wenqiangjiang bioinformaticidentificationandanalysesofthenonspecificlipidtransferproteinsinwheat AT jinghansong bioinformaticidentificationandanalysesofthenonspecificlipidtransferproteinsinwheat AT shupingwang bioinformaticidentificationandanalysesofthenonspecificlipidtransferproteinsinwheat AT dongfangma bioinformaticidentificationandanalysesofthenonspecificlipidtransferproteinsinwheat AT junliangyin bioinformaticidentificationandanalysesofthenonspecificlipidtransferproteinsinwheat |