Next-generation-sequencing technology used for the detection of Mycoplasma hominis in renal cyst fluid: a case report
Nan Xiao,1 Wei Gai,2 Wei-Guo Hu,1 Jian-Xing Li,1 Yan Zhang,2 Xiu-Ying Zhao11Department of Clinical Laboratory, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, Beijing, People’s Republic of China; 2National Engineering Research Center for Beijing Bioch...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2019-05-01
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| Series: | Infection and Drug Resistance |
| Subjects: | |
| Online Access: | https://www.dovepress.com/next-generation-sequencing-technology-used-for-the-detection-of-mycopl-peer-reviewed-article-IDR |
| _version_ | 1819073608334442496 |
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| author | Xiao N Gai W Hu WG Li JX Zhang Y Zhao XY |
| author_facet | Xiao N Gai W Hu WG Li JX Zhang Y Zhao XY |
| author_sort | Xiao N |
| collection | DOAJ |
| description | Nan Xiao,1 Wei Gai,2 Wei-Guo Hu,1 Jian-Xing Li,1 Yan Zhang,2 Xiu-Ying Zhao11Department of Clinical Laboratory, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, Beijing, People’s Republic of China; 2National Engineering Research Center for Beijing Biochip Technology, Beijing, People’s Republic of ChinaBackground: Mycoplasma is an opportunistic pathogen causing both urogenital and extragenital infections. The lack of cell wall renders Mycoplasma difficult to culture and identify with ordinary methods. Next-generation sequencing (NGS) is a new technology helping a lot in the diagnosis of infective diseases. In this case, NGS played a key role in the diagnosis of Mycoplasma infection.Case presentation: A mid-aged man suffering from renal cyst underwent cyst incision followed by invasive treatments to eliminate hematoma caused by renal artery hemorrhage. After the cyst incision operation, the patient had a persistent high temperature. The persistent increase of blood neutrophile granulocyte count and C-reaction protein suggested an unresolved infection. The empirically chosen anti-infective agents were meropenem and linezolid since the ordinary bacterial cultures of surgical site drainage and blood yielded a negative result. At postoperation day (POD) 17, NGS result of his drainage clearly indicated the pathogen was Mycoplasma hominis. At POD 24, the drug sensitivity test showed resistance to quinolones, clarithromycin and erythromycin, but intermediate to azithromycin. Since then, the antimicrobial agents were changed into azithromycin and kept unchanged until the patient was fully recovered and discharged at POD 39.Conclusion: When the ordinary laboratory diagnostic methods failed, NGS diagnosis could reduce the hospitalization expenses and shorten the lengths of hospital stay.Keywords: NGS, Mycoplasma, renal cyst, infection |
| first_indexed | 2024-12-21T17:56:19Z |
| format | Article |
| id | doaj.art-8d0238d68e664f7f80050e546621f684 |
| institution | Directory Open Access Journal |
| issn | 1178-6973 |
| language | English |
| last_indexed | 2024-12-21T17:56:19Z |
| publishDate | 2019-05-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Infection and Drug Resistance |
| spelling | doaj.art-8d0238d68e664f7f80050e546621f6842022-12-21T18:55:11ZengDove Medical PressInfection and Drug Resistance1178-69732019-05-01Volume 121073107945978Next-generation-sequencing technology used for the detection of Mycoplasma hominis in renal cyst fluid: a case reportXiao NGai WHu WGLi JXZhang YZhao XYNan Xiao,1 Wei Gai,2 Wei-Guo Hu,1 Jian-Xing Li,1 Yan Zhang,2 Xiu-Ying Zhao11Department of Clinical Laboratory, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, Beijing, People’s Republic of China; 2National Engineering Research Center for Beijing Biochip Technology, Beijing, People’s Republic of ChinaBackground: Mycoplasma is an opportunistic pathogen causing both urogenital and extragenital infections. The lack of cell wall renders Mycoplasma difficult to culture and identify with ordinary methods. Next-generation sequencing (NGS) is a new technology helping a lot in the diagnosis of infective diseases. In this case, NGS played a key role in the diagnosis of Mycoplasma infection.Case presentation: A mid-aged man suffering from renal cyst underwent cyst incision followed by invasive treatments to eliminate hematoma caused by renal artery hemorrhage. After the cyst incision operation, the patient had a persistent high temperature. The persistent increase of blood neutrophile granulocyte count and C-reaction protein suggested an unresolved infection. The empirically chosen anti-infective agents were meropenem and linezolid since the ordinary bacterial cultures of surgical site drainage and blood yielded a negative result. At postoperation day (POD) 17, NGS result of his drainage clearly indicated the pathogen was Mycoplasma hominis. At POD 24, the drug sensitivity test showed resistance to quinolones, clarithromycin and erythromycin, but intermediate to azithromycin. Since then, the antimicrobial agents were changed into azithromycin and kept unchanged until the patient was fully recovered and discharged at POD 39.Conclusion: When the ordinary laboratory diagnostic methods failed, NGS diagnosis could reduce the hospitalization expenses and shorten the lengths of hospital stay.Keywords: NGS, Mycoplasma, renal cyst, infectionhttps://www.dovepress.com/next-generation-sequencing-technology-used-for-the-detection-of-mycopl-peer-reviewed-article-IDRNGSMycoplasma hominisrenal cystcase report |
| spellingShingle | Xiao N Gai W Hu WG Li JX Zhang Y Zhao XY Next-generation-sequencing technology used for the detection of Mycoplasma hominis in renal cyst fluid: a case report Infection and Drug Resistance NGS Mycoplasma hominis renal cyst case report |
| title | Next-generation-sequencing technology used for the detection of Mycoplasma hominis in renal cyst fluid: a case report |
| title_full | Next-generation-sequencing technology used for the detection of Mycoplasma hominis in renal cyst fluid: a case report |
| title_fullStr | Next-generation-sequencing technology used for the detection of Mycoplasma hominis in renal cyst fluid: a case report |
| title_full_unstemmed | Next-generation-sequencing technology used for the detection of Mycoplasma hominis in renal cyst fluid: a case report |
| title_short | Next-generation-sequencing technology used for the detection of Mycoplasma hominis in renal cyst fluid: a case report |
| title_sort | next generation sequencing technology used for the detection of mycoplasma hominis in renal cyst fluid a case report |
| topic | NGS Mycoplasma hominis renal cyst case report |
| url | https://www.dovepress.com/next-generation-sequencing-technology-used-for-the-detection-of-mycopl-peer-reviewed-article-IDR |
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