Isolation and characterization of twelve polymorphic microsatellite markers in the endangered Hopea hainanensis (Dipterocarpaceae)
Abstract Microsatellite markers were isolated and characterized for Hopea hainanensis Merrill & Chun, an endangered tree species with scattered distribution in Hainan Island and northern Vietnam. Twenty‐six microsatellite markers were developed based on next‐generation sequencing data and were g...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Ecology and Evolution |
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| Online Access: | https://doi.org/10.1002/ece3.7077 |
| _version_ | 1819084928498794496 |
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| author | Chen Wang Xiang Ma Liang Tang |
| author_facet | Chen Wang Xiang Ma Liang Tang |
| author_sort | Chen Wang |
| collection | DOAJ |
| description | Abstract Microsatellite markers were isolated and characterized for Hopea hainanensis Merrill & Chun, an endangered tree species with scattered distribution in Hainan Island and northern Vietnam. Twenty‐six microsatellite markers were developed based on next‐generation sequencing data and were genotyped by capillary electrophoresis on an ABI 3730xl DNA Analyzer. Twelve markers were found to be polymorphic in H. hainanensis. GENODIVE analyses indicated that the number of alleles ranged from 2 to 6 per locus, and the observed and expected heterozygosity varied from 0 to 0.755 and from 0.259 to 0.779, respectively. Primer transferability was tested with Hopea chinensis Hand.‐Mazz. and Hopea reticulata Tardieu, in which 3 and 7 microsatellite markers were found to be polymorphic, separately. The results showed that H. reticulata and H. hainanensis had similar levels of genetic diversity. A neighbor joining dendrogram clustered all individuals into two major groups, one of which was exclusively constituted by H. hainanensis, while the other consisted of two subgroups, corresponding to H. reticulata and H. chinensis, respectively. The 12 polymorphic microsatellite markers could be applied to study genetic diversity, population differentiation, mating system, and fine‐scale spatial genetic structures of H. hainanensis as well as its close relatives, facilitating the conservation and restoration of these endangered but valuable Hopea species. |
| first_indexed | 2024-12-21T20:56:15Z |
| format | Article |
| id | doaj.art-8d03e7ce44e24a77bd85cf1644c44649 |
| institution | Directory Open Access Journal |
| issn | 2045-7758 |
| language | English |
| last_indexed | 2024-12-21T20:56:15Z |
| publishDate | 2021-01-01 |
| publisher | Wiley |
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| series | Ecology and Evolution |
| spelling | doaj.art-8d03e7ce44e24a77bd85cf1644c446492022-12-21T18:50:34ZengWileyEcology and Evolution2045-77582021-01-0111141010.1002/ece3.7077Isolation and characterization of twelve polymorphic microsatellite markers in the endangered Hopea hainanensis (Dipterocarpaceae)Chen Wang0Xiang Ma1Liang Tang2Key Laboratory of Tropical Biological Resources of Ministry of Education School of Life and Pharmaceutical Sciences Hainan University Haikou ChinaKey Laboratory of Tropical Biological Resources of Ministry of Education School of Life and Pharmaceutical Sciences Hainan University Haikou ChinaCollege of Ecology and Environment Hainan University Haikou ChinaAbstract Microsatellite markers were isolated and characterized for Hopea hainanensis Merrill & Chun, an endangered tree species with scattered distribution in Hainan Island and northern Vietnam. Twenty‐six microsatellite markers were developed based on next‐generation sequencing data and were genotyped by capillary electrophoresis on an ABI 3730xl DNA Analyzer. Twelve markers were found to be polymorphic in H. hainanensis. GENODIVE analyses indicated that the number of alleles ranged from 2 to 6 per locus, and the observed and expected heterozygosity varied from 0 to 0.755 and from 0.259 to 0.779, respectively. Primer transferability was tested with Hopea chinensis Hand.‐Mazz. and Hopea reticulata Tardieu, in which 3 and 7 microsatellite markers were found to be polymorphic, separately. The results showed that H. reticulata and H. hainanensis had similar levels of genetic diversity. A neighbor joining dendrogram clustered all individuals into two major groups, one of which was exclusively constituted by H. hainanensis, while the other consisted of two subgroups, corresponding to H. reticulata and H. chinensis, respectively. The 12 polymorphic microsatellite markers could be applied to study genetic diversity, population differentiation, mating system, and fine‐scale spatial genetic structures of H. hainanensis as well as its close relatives, facilitating the conservation and restoration of these endangered but valuable Hopea species.https://doi.org/10.1002/ece3.7077Dipterocarpaceaeendangered speciesH. hainanensismicrosatellite markersnext‐generation sequencing |
| spellingShingle | Chen Wang Xiang Ma Liang Tang Isolation and characterization of twelve polymorphic microsatellite markers in the endangered Hopea hainanensis (Dipterocarpaceae) Ecology and Evolution Dipterocarpaceae endangered species H. hainanensis microsatellite markers next‐generation sequencing |
| title | Isolation and characterization of twelve polymorphic microsatellite markers in the endangered Hopea hainanensis (Dipterocarpaceae) |
| title_full | Isolation and characterization of twelve polymorphic microsatellite markers in the endangered Hopea hainanensis (Dipterocarpaceae) |
| title_fullStr | Isolation and characterization of twelve polymorphic microsatellite markers in the endangered Hopea hainanensis (Dipterocarpaceae) |
| title_full_unstemmed | Isolation and characterization of twelve polymorphic microsatellite markers in the endangered Hopea hainanensis (Dipterocarpaceae) |
| title_short | Isolation and characterization of twelve polymorphic microsatellite markers in the endangered Hopea hainanensis (Dipterocarpaceae) |
| title_sort | isolation and characterization of twelve polymorphic microsatellite markers in the endangered hopea hainanensis dipterocarpaceae |
| topic | Dipterocarpaceae endangered species H. hainanensis microsatellite markers next‐generation sequencing |
| url | https://doi.org/10.1002/ece3.7077 |
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