mir-233 modulates the unfolded protein response in C. elegans during Pseudomonas aeruginosa infection.
The unfolded protein response (UPR), which is activated by perturbations of the endoplasmic reticulum homeostasis, has been shown to play an important role in innate immunity and inflammation. However, little is known about the molecular mechanisms underlying activation of the UPR during immune resp...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
|
| Series: | PLoS Pathogens |
| Online Access: | http://europepmc.org/articles/PMC4287614?pdf=render |
| _version_ | 1818440832385024000 |
|---|---|
| author | Li-Li Dai Jin-Xia Gao Cheng-Gang Zou Yi-Cheng Ma Ke-Qin Zhang |
| author_facet | Li-Li Dai Jin-Xia Gao Cheng-Gang Zou Yi-Cheng Ma Ke-Qin Zhang |
| author_sort | Li-Li Dai |
| collection | DOAJ |
| description | The unfolded protein response (UPR), which is activated by perturbations of the endoplasmic reticulum homeostasis, has been shown to play an important role in innate immunity and inflammation. However, little is known about the molecular mechanisms underlying activation of the UPR during immune responses. Using small RNA deep sequencing and reverse genetic analysis, we show that the microRNA mir-233 is required for activation of the UPR in Caenorhabditis elegans exposed to Pseudomonas aeruginosa PA14. P. aeruginosa infection up-regulates the expression of mir-233 in a p38 MAPK-dependent manner. Quantitative proteomic analysis identifies SCA-1, a C. elegans homologue of the sarco/endoplasmic reticulum Ca2+-ATPase, as a target of mir-233. During P. aeruginosa PA14 infection, mir-233 represses the protein levels of SCA-1, which in turn leads to activation of the UPR. Whereas mir-233 mutants are more sensitive to P. aeruginosa infection, knockdown of sca-1 leads to enhanced resistance to the killing by P. aeruginosa. Our study indicates that microRNA-dependent pathways may have an impact on innate immunity by activating the UPR. |
| first_indexed | 2024-12-14T18:18:37Z |
| format | Article |
| id | doaj.art-8d0493a5555c4efc9f6f09e655277c13 |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-12-14T18:18:37Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-8d0493a5555c4efc9f6f09e655277c132022-12-21T22:52:08ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742015-01-01111e100460610.1371/journal.ppat.1004606mir-233 modulates the unfolded protein response in C. elegans during Pseudomonas aeruginosa infection.Li-Li DaiJin-Xia GaoCheng-Gang ZouYi-Cheng MaKe-Qin ZhangThe unfolded protein response (UPR), which is activated by perturbations of the endoplasmic reticulum homeostasis, has been shown to play an important role in innate immunity and inflammation. However, little is known about the molecular mechanisms underlying activation of the UPR during immune responses. Using small RNA deep sequencing and reverse genetic analysis, we show that the microRNA mir-233 is required for activation of the UPR in Caenorhabditis elegans exposed to Pseudomonas aeruginosa PA14. P. aeruginosa infection up-regulates the expression of mir-233 in a p38 MAPK-dependent manner. Quantitative proteomic analysis identifies SCA-1, a C. elegans homologue of the sarco/endoplasmic reticulum Ca2+-ATPase, as a target of mir-233. During P. aeruginosa PA14 infection, mir-233 represses the protein levels of SCA-1, which in turn leads to activation of the UPR. Whereas mir-233 mutants are more sensitive to P. aeruginosa infection, knockdown of sca-1 leads to enhanced resistance to the killing by P. aeruginosa. Our study indicates that microRNA-dependent pathways may have an impact on innate immunity by activating the UPR.http://europepmc.org/articles/PMC4287614?pdf=render |
| spellingShingle | Li-Li Dai Jin-Xia Gao Cheng-Gang Zou Yi-Cheng Ma Ke-Qin Zhang mir-233 modulates the unfolded protein response in C. elegans during Pseudomonas aeruginosa infection. PLoS Pathogens |
| title | mir-233 modulates the unfolded protein response in C. elegans during Pseudomonas aeruginosa infection. |
| title_full | mir-233 modulates the unfolded protein response in C. elegans during Pseudomonas aeruginosa infection. |
| title_fullStr | mir-233 modulates the unfolded protein response in C. elegans during Pseudomonas aeruginosa infection. |
| title_full_unstemmed | mir-233 modulates the unfolded protein response in C. elegans during Pseudomonas aeruginosa infection. |
| title_short | mir-233 modulates the unfolded protein response in C. elegans during Pseudomonas aeruginosa infection. |
| title_sort | mir 233 modulates the unfolded protein response in c elegans during pseudomonas aeruginosa infection |
| url | http://europepmc.org/articles/PMC4287614?pdf=render |
| work_keys_str_mv | AT lilidai mir233modulatestheunfoldedproteinresponseincelegansduringpseudomonasaeruginosainfection AT jinxiagao mir233modulatestheunfoldedproteinresponseincelegansduringpseudomonasaeruginosainfection AT chenggangzou mir233modulatestheunfoldedproteinresponseincelegansduringpseudomonasaeruginosainfection AT yichengma mir233modulatestheunfoldedproteinresponseincelegansduringpseudomonasaeruginosainfection AT keqinzhang mir233modulatestheunfoldedproteinresponseincelegansduringpseudomonasaeruginosainfection |