Rapid construction of metabolite biosensors using domain-insertion profiling
In the construction of single fluorescent protein biosensors, selection of the insertion point of a fluorescent protein into a ligand-binding domain is a rate-limiting step. Here, the authors develop an unbiased, high-throughput approach, called domain insertion profiling with DNA sequencing (DIP-se...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2016-07-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/ncomms12266 |
| _version_ | 1818716863871320064 |
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| author | Dana C. Nadler Stacy-Anne Morgan Avi Flamholz Kaitlyn E. Kortright David F. Savage |
| author_facet | Dana C. Nadler Stacy-Anne Morgan Avi Flamholz Kaitlyn E. Kortright David F. Savage |
| author_sort | Dana C. Nadler |
| collection | DOAJ |
| description | In the construction of single fluorescent protein biosensors, selection of the insertion point of a fluorescent protein into a ligand-binding domain is a rate-limiting step. Here, the authors develop an unbiased, high-throughput approach, called domain insertion profiling with DNA sequencing (DIP-seq), to generate a novel trehalose biosensor. |
| first_indexed | 2024-12-17T19:26:01Z |
| format | Article |
| id | doaj.art-8d05fc327b244948b82af826e79987c9 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-17T19:26:01Z |
| publishDate | 2016-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-8d05fc327b244948b82af826e79987c92022-12-21T21:35:23ZengNature PortfolioNature Communications2041-17232016-07-017111110.1038/ncomms12266Rapid construction of metabolite biosensors using domain-insertion profilingDana C. Nadler0Stacy-Anne Morgan1Avi Flamholz2Kaitlyn E. Kortright3David F. Savage4Department of Molecular & Cell Biology, University of CaliforniaDepartment of Molecular & Cell Biology, University of CaliforniaDepartment of Molecular & Cell Biology, University of CaliforniaDepartment of Molecular & Cell Biology, University of CaliforniaDepartment of Molecular & Cell Biology, University of CaliforniaIn the construction of single fluorescent protein biosensors, selection of the insertion point of a fluorescent protein into a ligand-binding domain is a rate-limiting step. Here, the authors develop an unbiased, high-throughput approach, called domain insertion profiling with DNA sequencing (DIP-seq), to generate a novel trehalose biosensor.https://doi.org/10.1038/ncomms12266 |
| spellingShingle | Dana C. Nadler Stacy-Anne Morgan Avi Flamholz Kaitlyn E. Kortright David F. Savage Rapid construction of metabolite biosensors using domain-insertion profiling Nature Communications |
| title | Rapid construction of metabolite biosensors using domain-insertion profiling |
| title_full | Rapid construction of metabolite biosensors using domain-insertion profiling |
| title_fullStr | Rapid construction of metabolite biosensors using domain-insertion profiling |
| title_full_unstemmed | Rapid construction of metabolite biosensors using domain-insertion profiling |
| title_short | Rapid construction of metabolite biosensors using domain-insertion profiling |
| title_sort | rapid construction of metabolite biosensors using domain insertion profiling |
| url | https://doi.org/10.1038/ncomms12266 |
| work_keys_str_mv | AT danacnadler rapidconstructionofmetabolitebiosensorsusingdomaininsertionprofiling AT stacyannemorgan rapidconstructionofmetabolitebiosensorsusingdomaininsertionprofiling AT aviflamholz rapidconstructionofmetabolitebiosensorsusingdomaininsertionprofiling AT kaitlynekortright rapidconstructionofmetabolitebiosensorsusingdomaininsertionprofiling AT davidfsavage rapidconstructionofmetabolitebiosensorsusingdomaininsertionprofiling |