.Study of adsorption of factor vVIIIVFW complex on ion-exchange supports.
We investigated the binding capacity for FVIII/vWF complex of five different anion-exchange supports, DEAE-Toyopearl 650M, Toyopearl Super Q-650M, Fractogel EMD TMAE (M), DEAE Sepharose FF, Fractogel EMD TMAE Hicap (M). The chromatographic supports with tentacle structure demonstrated the best resul...
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| Format: | Article |
| Language: | Russian |
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MIREA - Russian Technological University
2009-10-01
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| Series: | Тонкие химические технологии |
| Subjects: | |
| Online Access: | https://www.finechem-mirea.ru/jour/article/view/1053 |
| _version_ | 1797882190389837824 |
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| author | M. A. Azighirova T. L. Dereza O. G. Kuturova T. A. Zipileva |
| author_facet | M. A. Azighirova T. L. Dereza O. G. Kuturova T. A. Zipileva |
| author_sort | M. A. Azighirova |
| collection | DOAJ |
| description | We investigated the binding capacity for FVIII/vWF complex of five different anion-exchange supports, DEAE-Toyopearl 650M, Toyopearl Super Q-650M, Fractogel EMD TMAE (M), DEAE Sepharose FF, Fractogel EMD TMAE Hicap (M). The chromatographic supports with tentacle structure demonstrated the best results. The adsorption was performed by a batch-method when mixing at the room t0 and a residual FVIII and vWF-content was measured in the supernatant. The maximum binding capacity was estimated 188 IU FVIII /g and 2309 IU vWF /g for Fractogel EMD TMAE (M) and 134 IU FVIII /g and 1935 IU vWF /g for Fractogel EMD TMAE Hicap (M). The results obtained may be used for development of the method of purifying a FVIII/vWF complex using ion-exchange chromatography. |
| first_indexed | 2024-04-10T03:32:03Z |
| format | Article |
| id | doaj.art-8d0aa97460764ed5b39bcf8d27686214 |
| institution | Directory Open Access Journal |
| issn | 2410-6593 2686-7575 |
| language | Russian |
| last_indexed | 2024-04-10T03:32:03Z |
| publishDate | 2009-10-01 |
| publisher | MIREA - Russian Technological University |
| record_format | Article |
| series | Тонкие химические технологии |
| spelling | doaj.art-8d0aa97460764ed5b39bcf8d276862142023-03-13T07:25:30ZrusMIREA - Russian Technological UniversityТонкие химические технологии2410-65932686-75752009-10-014520241047.Study of adsorption of factor vVIIIVFW complex on ion-exchange supports.M. A. Azighirova0T. L. Dereza1O. G. Kuturova2T. A. Zipileva3Учреждение Российской академии медицинских наукУчреждение Российской академии медицинских наукУчреждение Российской академии медицинских наукУчреждение Российской академии медицинских наукWe investigated the binding capacity for FVIII/vWF complex of five different anion-exchange supports, DEAE-Toyopearl 650M, Toyopearl Super Q-650M, Fractogel EMD TMAE (M), DEAE Sepharose FF, Fractogel EMD TMAE Hicap (M). The chromatographic supports with tentacle structure demonstrated the best results. The adsorption was performed by a batch-method when mixing at the room t0 and a residual FVIII and vWF-content was measured in the supernatant. The maximum binding capacity was estimated 188 IU FVIII /g and 2309 IU vWF /g for Fractogel EMD TMAE (M) and 134 IU FVIII /g and 1935 IU vWF /g for Fractogel EMD TMAE Hicap (M). The results obtained may be used for development of the method of purifying a FVIII/vWF complex using ion-exchange chromatography.https://www.finechem-mirea.ru/jour/article/view/1053coagulation blood factors, sorbents for chromatography, cryoprecipitate |
| spellingShingle | M. A. Azighirova T. L. Dereza O. G. Kuturova T. A. Zipileva .Study of adsorption of factor vVIIIVFW complex on ion-exchange supports. Тонкие химические технологии coagulation blood factors, sorbents for chromatography, cryoprecipitate |
| title | .Study of adsorption of factor vVIIIVFW complex on ion-exchange supports. |
| title_full | .Study of adsorption of factor vVIIIVFW complex on ion-exchange supports. |
| title_fullStr | .Study of adsorption of factor vVIIIVFW complex on ion-exchange supports. |
| title_full_unstemmed | .Study of adsorption of factor vVIIIVFW complex on ion-exchange supports. |
| title_short | .Study of adsorption of factor vVIIIVFW complex on ion-exchange supports. |
| title_sort | study of adsorption of factor vviiivfw complex on ion exchange supports |
| topic | coagulation blood factors, sorbents for chromatography, cryoprecipitate |
| url | https://www.finechem-mirea.ru/jour/article/view/1053 |
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