EVA1A/TMEM166 Regulates Embryonic Neurogenesis by Autophagy
Self-renewal and differentiation of neural stem cells is essential for embryonic neurogenesis, which is associated with cell autophagy. However, the mechanism by which autophagy regulates neurogenesis remains undefined. Here, we show that Eva1a/Tmem166, an autophagy-related gene, regulates neural st...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016-03-01
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| Series: | Stem Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S221367111600028X |
| _version_ | 1811192607703826432 |
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| author | Mengtao Li Guang Lu Jia Hu Xue Shen Jiabao Ju Yuanxu Gao Liujing Qu Yan Xia Yingyu Chen Yun Bai |
| author_facet | Mengtao Li Guang Lu Jia Hu Xue Shen Jiabao Ju Yuanxu Gao Liujing Qu Yan Xia Yingyu Chen Yun Bai |
| author_sort | Mengtao Li |
| collection | DOAJ |
| description | Self-renewal and differentiation of neural stem cells is essential for embryonic neurogenesis, which is associated with cell autophagy. However, the mechanism by which autophagy regulates neurogenesis remains undefined. Here, we show that Eva1a/Tmem166, an autophagy-related gene, regulates neural stem cell self-renewal and differentiation. Eva1a depletion impaired the generation of newborn neurons, both in vivo and in vitro. Conversely, overexpression of EVA1A enhanced newborn neuron generation and maturation. Moreover, Eva1a depletion activated the PIK3CA-AKT axis, leading to the activation of the mammalian target of rapamycin and the subsequent inhibition of autophagy. Furthermore, addition of methylpyruvate to the culture during neural stem cell differentiation rescued the defective embryonic neurogenesis induced by Eva1a depletion, suggesting that energy availability is a significant factor in embryonic neurogenesis. Collectively, these data demonstrated that EVA1A regulates embryonic neurogenesis by modulating autophagy. Our results have potential implications for understanding the pathogenesis of neurodevelopmental disorders caused by autophagy dysregulation. |
| first_indexed | 2024-04-11T23:55:17Z |
| format | Article |
| id | doaj.art-8d0bb4542d374bc68376e2faf00c3bd3 |
| institution | Directory Open Access Journal |
| issn | 2213-6711 |
| language | English |
| last_indexed | 2024-04-11T23:55:17Z |
| publishDate | 2016-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Reports |
| spelling | doaj.art-8d0bb4542d374bc68376e2faf00c3bd32022-12-22T03:56:23ZengElsevierStem Cell Reports2213-67112016-03-016339641010.1016/j.stemcr.2016.01.011EVA1A/TMEM166 Regulates Embryonic Neurogenesis by AutophagyMengtao Li0Guang Lu1Jia Hu2Xue Shen3Jiabao Ju4Yuanxu Gao5Liujing Qu6Yan Xia7Yingyu Chen8Yun Bai9Department of Cell Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, ChinaDepartment of Cell Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, ChinaDepartment of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, ChinaDepartment of Cell Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, ChinaDepartment of Cell Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, ChinaDepartment of Cell Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, ChinaDepartment of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, ChinaDepartment of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, ChinaDepartment of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, ChinaDepartment of Cell Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, ChinaSelf-renewal and differentiation of neural stem cells is essential for embryonic neurogenesis, which is associated with cell autophagy. However, the mechanism by which autophagy regulates neurogenesis remains undefined. Here, we show that Eva1a/Tmem166, an autophagy-related gene, regulates neural stem cell self-renewal and differentiation. Eva1a depletion impaired the generation of newborn neurons, both in vivo and in vitro. Conversely, overexpression of EVA1A enhanced newborn neuron generation and maturation. Moreover, Eva1a depletion activated the PIK3CA-AKT axis, leading to the activation of the mammalian target of rapamycin and the subsequent inhibition of autophagy. Furthermore, addition of methylpyruvate to the culture during neural stem cell differentiation rescued the defective embryonic neurogenesis induced by Eva1a depletion, suggesting that energy availability is a significant factor in embryonic neurogenesis. Collectively, these data demonstrated that EVA1A regulates embryonic neurogenesis by modulating autophagy. Our results have potential implications for understanding the pathogenesis of neurodevelopmental disorders caused by autophagy dysregulation.http://www.sciencedirect.com/science/article/pii/S221367111600028Xneural stem cellsneurogenesisautophagyEva1aneurodevelopmental disorder |
| spellingShingle | Mengtao Li Guang Lu Jia Hu Xue Shen Jiabao Ju Yuanxu Gao Liujing Qu Yan Xia Yingyu Chen Yun Bai EVA1A/TMEM166 Regulates Embryonic Neurogenesis by Autophagy Stem Cell Reports neural stem cells neurogenesis autophagy Eva1a neurodevelopmental disorder |
| title | EVA1A/TMEM166 Regulates Embryonic Neurogenesis by Autophagy |
| title_full | EVA1A/TMEM166 Regulates Embryonic Neurogenesis by Autophagy |
| title_fullStr | EVA1A/TMEM166 Regulates Embryonic Neurogenesis by Autophagy |
| title_full_unstemmed | EVA1A/TMEM166 Regulates Embryonic Neurogenesis by Autophagy |
| title_short | EVA1A/TMEM166 Regulates Embryonic Neurogenesis by Autophagy |
| title_sort | eva1a tmem166 regulates embryonic neurogenesis by autophagy |
| topic | neural stem cells neurogenesis autophagy Eva1a neurodevelopmental disorder |
| url | http://www.sciencedirect.com/science/article/pii/S221367111600028X |
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