Specific and sensitive detection tools for Xanthomonas arboricola pv. corylina, the causal agent of bacterial blight of hazelnut, developed with comparative genomics
Xanthomonas arboricola pv. corylina (Xac; formerly Xanthomonas campestris pv. corylina) is the causal agent of the bacterial blight of hazelnuts, a devastating disease of trees in plant nurseries and young orchards. Currently, there are no PCR assays to distinguish Xac from all other pathovars of X....
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1254107/full |
| _version_ | 1797687132299460608 |
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| author | Monika Kałużna Andjelka Prokić Aleksa Obradović William A. Weldon Virginia O. Stockwell Joël F. Pothier |
| author_facet | Monika Kałużna Andjelka Prokić Aleksa Obradović William A. Weldon Virginia O. Stockwell Joël F. Pothier |
| author_sort | Monika Kałużna |
| collection | DOAJ |
| description | Xanthomonas arboricola pv. corylina (Xac; formerly Xanthomonas campestris pv. corylina) is the causal agent of the bacterial blight of hazelnuts, a devastating disease of trees in plant nurseries and young orchards. Currently, there are no PCR assays to distinguish Xac from all other pathovars of X. arboricola. A comparative genomics approach with publicly available genomes of Xac was used to identify unique sequences, conserved across the genomes of the pathogen. We identified a 2,440 bp genomic region that was unique to Xac and designed identification and detection systems for conventional PCR, qPCR (SYBR® Green and TaqMan™), and loop-mediated isothermal amplification (LAMP). All PCR assays performed on genomic DNA isolated from eight X. arboricola pathovars and closely related bacterial species confirmed the specificity of designed primers. These new multi-platform molecular diagnostic tools may be used by plant clinics and researchers to detect and identify Xac in pure cultures and hazelnut tissues rapidly and accurately. |
| first_indexed | 2024-03-12T01:13:53Z |
| format | Article |
| id | doaj.art-8d0c1df57e1443488ab01975c164a8ad |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-12T01:13:53Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-8d0c1df57e1443488ab01975c164a8ad2023-09-13T21:55:00ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-09-011410.3389/fpls.2023.12541071254107Specific and sensitive detection tools for Xanthomonas arboricola pv. corylina, the causal agent of bacterial blight of hazelnut, developed with comparative genomicsMonika Kałużna0Andjelka Prokić1Aleksa Obradović2William A. Weldon3Virginia O. Stockwell4Joël F. Pothier5The National Institute of Horticultural Research, Skierniewice, PolandUniversity of Belgrade, Faculty of Agriculture, Belgrade, SerbiaUniversity of Belgrade, Faculty of Agriculture, Belgrade, SerbiaValent BioSciences, Libertyville, IL, United StatesUnited States Department of Agriculture, Agricultural Research Service, Horticultural Crops Disease and Pest Management Research Unit, Corvallis, OR, United StatesEnvironmental Genomics and Systems Biology Research Group, Institute for Natural Resource Sciences, Zurich University of Applied Sciences (ZHAW), Wädenswil, SwitzerlandXanthomonas arboricola pv. corylina (Xac; formerly Xanthomonas campestris pv. corylina) is the causal agent of the bacterial blight of hazelnuts, a devastating disease of trees in plant nurseries and young orchards. Currently, there are no PCR assays to distinguish Xac from all other pathovars of X. arboricola. A comparative genomics approach with publicly available genomes of Xac was used to identify unique sequences, conserved across the genomes of the pathogen. We identified a 2,440 bp genomic region that was unique to Xac and designed identification and detection systems for conventional PCR, qPCR (SYBR® Green and TaqMan™), and loop-mediated isothermal amplification (LAMP). All PCR assays performed on genomic DNA isolated from eight X. arboricola pathovars and closely related bacterial species confirmed the specificity of designed primers. These new multi-platform molecular diagnostic tools may be used by plant clinics and researchers to detect and identify Xac in pure cultures and hazelnut tissues rapidly and accurately.https://www.frontiersin.org/articles/10.3389/fpls.2023.1254107/fullCorylus spp.Corylus avellanadiagnosisPCRLAMPqPCR |
| spellingShingle | Monika Kałużna Andjelka Prokić Aleksa Obradović William A. Weldon Virginia O. Stockwell Joël F. Pothier Specific and sensitive detection tools for Xanthomonas arboricola pv. corylina, the causal agent of bacterial blight of hazelnut, developed with comparative genomics Frontiers in Plant Science Corylus spp. Corylus avellana diagnosis PCR LAMP qPCR |
| title | Specific and sensitive detection tools for Xanthomonas arboricola pv. corylina, the causal agent of bacterial blight of hazelnut, developed with comparative genomics |
| title_full | Specific and sensitive detection tools for Xanthomonas arboricola pv. corylina, the causal agent of bacterial blight of hazelnut, developed with comparative genomics |
| title_fullStr | Specific and sensitive detection tools for Xanthomonas arboricola pv. corylina, the causal agent of bacterial blight of hazelnut, developed with comparative genomics |
| title_full_unstemmed | Specific and sensitive detection tools for Xanthomonas arboricola pv. corylina, the causal agent of bacterial blight of hazelnut, developed with comparative genomics |
| title_short | Specific and sensitive detection tools for Xanthomonas arboricola pv. corylina, the causal agent of bacterial blight of hazelnut, developed with comparative genomics |
| title_sort | specific and sensitive detection tools for xanthomonas arboricola pv corylina the causal agent of bacterial blight of hazelnut developed with comparative genomics |
| topic | Corylus spp. Corylus avellana diagnosis PCR LAMP qPCR |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1254107/full |
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