The Accuracy of Serum Biomarkers in the Diagnosis of Steatosis, Fibrosis, and Inflammation in Patients with Nonalcoholic Fatty Liver Disease in Comparison to a Liver Biopsy
<i>Background and Objective</i>: This study was conducted to evaluate the diagnostic performance of various biomarkers for steatosis, fibrosis, and inflammation in comparison to a liver biopsy (LB) in patients with nonalcoholic fatty liver disease (NAFLD). <i>Materials and Methods:...
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MDPI AG
2022-02-01
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| Online Access: | https://www.mdpi.com/1648-9144/58/2/252 |
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| author | Ivana Mikolasevic Viktor Domislovic Irena Krznaric-Zrnic Zeljko Krznaric Lucija Virovic-Jukic Sanja Stojsavljevic Ivica Grgurevic Sandra Milic Ivan Vukoja Petra Puz Merica Aralica Goran Hauser |
| author_facet | Ivana Mikolasevic Viktor Domislovic Irena Krznaric-Zrnic Zeljko Krznaric Lucija Virovic-Jukic Sanja Stojsavljevic Ivica Grgurevic Sandra Milic Ivan Vukoja Petra Puz Merica Aralica Goran Hauser |
| author_sort | Ivana Mikolasevic |
| collection | DOAJ |
| description | <i>Background and Objective</i>: This study was conducted to evaluate the diagnostic performance of various biomarkers for steatosis, fibrosis, and inflammation in comparison to a liver biopsy (LB) in patients with nonalcoholic fatty liver disease (NAFLD). <i>Materials and Methods:</i> This was a cross-sectional study that included 135 patients with biopsy-proven NAFLD. Fatty liver index (FLI), hepatic steatosis index (HSI), cell death markers (CK-18 M30 and CK-18 M65), FIB-4 index, NAFLD fibrosis score (NFS), BARD, and AST to platelet ratio index (APRI) were calculated and analysed. <i>Results:</i> FLI, HSI scores, and the cell death biomarkers showed poor diagnostic accuracy for steatosis detection and quantification, with an area under the curve (AUC) of <0.70. The cell death biomarkers likewise did not perform well for the detection of nonalcoholic steatohepatitis (NASH) (AUC < 0.7). As for the fibrosis staging, only APRI and the cell death biomarkers had moderate accuracy (AUC > 0.7) for advanced fibrosis, whereas FIB-4, BARD, and NFS scores demonstrated poor performance (AUC < 0.70). However, a combination of FIB-4 and NFS with the cell death biomarkers had moderate accuracy for advanced (≥F3) fibrosis detection, with an AUC of >0.70. <i>Conclusions:</i> In this first study on Croatian patients with NAFLD, serum biomarkers demonstrated poor diagnostic performance for the noninvasive diagnosis of liver steatosis and NASH. APRI and the cell death biomarkers had only moderate accuracy for diagnosing advanced fibrosis, as did the combination of FIB-4 and NFS with the cell death biomarkers. Further studies regarding serum biomarkers for all NAFLD stages are needed. |
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| issn | 1010-660X 1648-9144 |
| language | English |
| last_indexed | 2024-03-09T21:29:39Z |
| publishDate | 2022-02-01 |
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| spelling | doaj.art-8d0f214beac849c1af6126ab0b1fd21c2023-11-23T21:00:25ZengMDPI AGMedicina1010-660X1648-91442022-02-0158225210.3390/medicina58020252The Accuracy of Serum Biomarkers in the Diagnosis of Steatosis, Fibrosis, and Inflammation in Patients with Nonalcoholic Fatty Liver Disease in Comparison to a Liver BiopsyIvana Mikolasevic0Viktor Domislovic1Irena Krznaric-Zrnic2Zeljko Krznaric3Lucija Virovic-Jukic4Sanja Stojsavljevic5Ivica Grgurevic6Sandra Milic7Ivan Vukoja8Petra Puz9Merica Aralica10Goran Hauser11Department of Gastroenterology, University Hospital Center Rijeka, 51000 Rijeka, CroatiaDepartment for Gastroenterology and Hepatology, University Hospital Center Zagreb, 10000 Zagreb, CroatiaDepartment of Gastroenterology, University Hospital Center Rijeka, 51000 Rijeka, CroatiaDepartment for Gastroenterology and Hepatology, University Hospital Center Zagreb, 10000 Zagreb, CroatiaFaculty of Medicine, 10000 Zagreb, CroatiaDepartment of Gastroenterology, University Hospital Center “Sestre Milosrdnice”, 10000 Zagreb, CroatiaFaculty of Medicine, 10000 Zagreb, CroatiaDepartment of Gastroenterology, University Hospital Center Rijeka, 51000 Rijeka, CroatiaDepartment of Internal medicine, General Hospital Pozega, 34000 Požega, CroatiaDepartment of Internal medicine, General Hospital Koprivnica, 48000 Koprivnica, CroatiaClinical Institute for Laboratory Diagnostics, Clinical Hospital Centre, 51000 Rijeka, CroatiaDepartment of Gastroenterology, University Hospital Center Rijeka, 51000 Rijeka, Croatia<i>Background and Objective</i>: This study was conducted to evaluate the diagnostic performance of various biomarkers for steatosis, fibrosis, and inflammation in comparison to a liver biopsy (LB) in patients with nonalcoholic fatty liver disease (NAFLD). <i>Materials and Methods:</i> This was a cross-sectional study that included 135 patients with biopsy-proven NAFLD. Fatty liver index (FLI), hepatic steatosis index (HSI), cell death markers (CK-18 M30 and CK-18 M65), FIB-4 index, NAFLD fibrosis score (NFS), BARD, and AST to platelet ratio index (APRI) were calculated and analysed. <i>Results:</i> FLI, HSI scores, and the cell death biomarkers showed poor diagnostic accuracy for steatosis detection and quantification, with an area under the curve (AUC) of <0.70. The cell death biomarkers likewise did not perform well for the detection of nonalcoholic steatohepatitis (NASH) (AUC < 0.7). As for the fibrosis staging, only APRI and the cell death biomarkers had moderate accuracy (AUC > 0.7) for advanced fibrosis, whereas FIB-4, BARD, and NFS scores demonstrated poor performance (AUC < 0.70). However, a combination of FIB-4 and NFS with the cell death biomarkers had moderate accuracy for advanced (≥F3) fibrosis detection, with an AUC of >0.70. <i>Conclusions:</i> In this first study on Croatian patients with NAFLD, serum biomarkers demonstrated poor diagnostic performance for the noninvasive diagnosis of liver steatosis and NASH. APRI and the cell death biomarkers had only moderate accuracy for diagnosing advanced fibrosis, as did the combination of FIB-4 and NFS with the cell death biomarkers. Further studies regarding serum biomarkers for all NAFLD stages are needed.https://www.mdpi.com/1648-9144/58/2/252nonalcoholic fatty liver diseasebiomarkerssteatosisfibrosisinflammation |
| spellingShingle | Ivana Mikolasevic Viktor Domislovic Irena Krznaric-Zrnic Zeljko Krznaric Lucija Virovic-Jukic Sanja Stojsavljevic Ivica Grgurevic Sandra Milic Ivan Vukoja Petra Puz Merica Aralica Goran Hauser The Accuracy of Serum Biomarkers in the Diagnosis of Steatosis, Fibrosis, and Inflammation in Patients with Nonalcoholic Fatty Liver Disease in Comparison to a Liver Biopsy Medicina nonalcoholic fatty liver disease biomarkers steatosis fibrosis inflammation |
| title | The Accuracy of Serum Biomarkers in the Diagnosis of Steatosis, Fibrosis, and Inflammation in Patients with Nonalcoholic Fatty Liver Disease in Comparison to a Liver Biopsy |
| title_full | The Accuracy of Serum Biomarkers in the Diagnosis of Steatosis, Fibrosis, and Inflammation in Patients with Nonalcoholic Fatty Liver Disease in Comparison to a Liver Biopsy |
| title_fullStr | The Accuracy of Serum Biomarkers in the Diagnosis of Steatosis, Fibrosis, and Inflammation in Patients with Nonalcoholic Fatty Liver Disease in Comparison to a Liver Biopsy |
| title_full_unstemmed | The Accuracy of Serum Biomarkers in the Diagnosis of Steatosis, Fibrosis, and Inflammation in Patients with Nonalcoholic Fatty Liver Disease in Comparison to a Liver Biopsy |
| title_short | The Accuracy of Serum Biomarkers in the Diagnosis of Steatosis, Fibrosis, and Inflammation in Patients with Nonalcoholic Fatty Liver Disease in Comparison to a Liver Biopsy |
| title_sort | accuracy of serum biomarkers in the diagnosis of steatosis fibrosis and inflammation in patients with nonalcoholic fatty liver disease in comparison to a liver biopsy |
| topic | nonalcoholic fatty liver disease biomarkers steatosis fibrosis inflammation |
| url | https://www.mdpi.com/1648-9144/58/2/252 |
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