Response surface methodology-optimized extraction of flavonoids from pomelo peels and isolation of naringin with antioxidant activities by Sephadex LH20 gel chromatography
In this study, flavonoids were extracted from pomelo peels and naringin was isolated from the flavonoid extract. The effects of extraction parameters, namely, ethanol concentration, solid-to-liquid ratio, and extraction time, on the yield of flavonoids extracted from pomelo peels were analyzed accor...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-01-01
|
| Series: | Current Research in Food Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2665927123001788 |
| _version_ | 1827587886223982592 |
|---|---|
| author | Yuchen Shangguan Jing Ni Lili Jiang Yang Hu Chuanbo He Ying Ma Guohong Wu Hejian Xiong |
| author_facet | Yuchen Shangguan Jing Ni Lili Jiang Yang Hu Chuanbo He Ying Ma Guohong Wu Hejian Xiong |
| author_sort | Yuchen Shangguan |
| collection | DOAJ |
| description | In this study, flavonoids were extracted from pomelo peels and naringin was isolated from the flavonoid extract. The effects of extraction parameters, namely, ethanol concentration, solid-to-liquid ratio, and extraction time, on the yield of flavonoids extracted from pomelo peels were analyzed according to the Box-Behnken design of response surface methodology. The experimental conditions for flavonoid extraction were optimized, and naringin was separated from the extracted flavonoids using Sephadex LH-20 column chromatography. Experimental results showed that the influence of factors on the extraction rate of flavonoids from pomelo peels was in the order of ethanol concentration > solid-to-liquid ratio > extraction time, and the optimal extraction parameters were 85% ethanol concentration, 1:20 solid-to-liquid ratio, and 4-h extraction time for extracting flavonoids from pomelo peels. Under these conditions, the yield of flavonoids was 6.07 ± 0.06 mg/g. After three times of extraction, the flavonoid extraction rate reached 96.55%, and the residual naringin in the pomelo peels was 0.017 mg/g, at which point the bitterness in the pomelo peels disappeared. Two components, namely, PF1 and PF2, were separated from the crude flavonoid of pomelo peels through Sephadex LH20 column chromatography. PF2 was identified as naringin by high-performance liquid chromatography tandem mass spectrometry, with a purity of 95.7 ± 0.23%. Both flavonoids and PF2 exhibited good in vitro radicals scavenging activities on DPPH, ABTS, superoxide anion and hydroxyl. |
| first_indexed | 2024-03-09T00:25:29Z |
| format | Article |
| id | doaj.art-8d1a33a284df4e08ba19c797c70bef73 |
| institution | Directory Open Access Journal |
| issn | 2665-9271 |
| language | English |
| last_indexed | 2024-03-09T00:25:29Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Food Science |
| spelling | doaj.art-8d1a33a284df4e08ba19c797c70bef732023-12-12T04:36:08ZengElsevierCurrent Research in Food Science2665-92712023-01-017100610Response surface methodology-optimized extraction of flavonoids from pomelo peels and isolation of naringin with antioxidant activities by Sephadex LH20 gel chromatographyYuchen Shangguan0Jing Ni1Lili Jiang2Yang Hu3Chuanbo He4Ying Ma5Guohong Wu6Hejian Xiong7College of Ocean Food and Biological Engineering, Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Jimei University, Xiamen, 361021, China; Jiangle County Agricultural Products Quality and Safety Inspection Station, Sanming, 353300, ChinaFisheries College of Jimei University, Xiamen, 361021, ChinaXiamen Municipal Southern Ocean Testing Co., L, Xiamen, 361021, ChinaCollege of Ocean Food and Biological Engineering, Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Jimei University, Xiamen, 361021, ChinaCollege of Ocean Food and Biological Engineering, Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Jimei University, Xiamen, 361021, ChinaFisheries College of Jimei University, Xiamen, 361021, ChinaCollege of Ocean Food and Biological Engineering, Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Jimei University, Xiamen, 361021, China; Corresponding author.College of Ocean Food and Biological Engineering, Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Jimei University, Xiamen, 361021, China; Corresponding author.In this study, flavonoids were extracted from pomelo peels and naringin was isolated from the flavonoid extract. The effects of extraction parameters, namely, ethanol concentration, solid-to-liquid ratio, and extraction time, on the yield of flavonoids extracted from pomelo peels were analyzed according to the Box-Behnken design of response surface methodology. The experimental conditions for flavonoid extraction were optimized, and naringin was separated from the extracted flavonoids using Sephadex LH-20 column chromatography. Experimental results showed that the influence of factors on the extraction rate of flavonoids from pomelo peels was in the order of ethanol concentration > solid-to-liquid ratio > extraction time, and the optimal extraction parameters were 85% ethanol concentration, 1:20 solid-to-liquid ratio, and 4-h extraction time for extracting flavonoids from pomelo peels. Under these conditions, the yield of flavonoids was 6.07 ± 0.06 mg/g. After three times of extraction, the flavonoid extraction rate reached 96.55%, and the residual naringin in the pomelo peels was 0.017 mg/g, at which point the bitterness in the pomelo peels disappeared. Two components, namely, PF1 and PF2, were separated from the crude flavonoid of pomelo peels through Sephadex LH20 column chromatography. PF2 was identified as naringin by high-performance liquid chromatography tandem mass spectrometry, with a purity of 95.7 ± 0.23%. Both flavonoids and PF2 exhibited good in vitro radicals scavenging activities on DPPH, ABTS, superoxide anion and hydroxyl.http://www.sciencedirect.com/science/article/pii/S2665927123001788FlavonoidsPomelo peelsSephadex LH-20Response surface methodologyDebittering |
| spellingShingle | Yuchen Shangguan Jing Ni Lili Jiang Yang Hu Chuanbo He Ying Ma Guohong Wu Hejian Xiong Response surface methodology-optimized extraction of flavonoids from pomelo peels and isolation of naringin with antioxidant activities by Sephadex LH20 gel chromatography Current Research in Food Science Flavonoids Pomelo peels Sephadex LH-20 Response surface methodology Debittering |
| title | Response surface methodology-optimized extraction of flavonoids from pomelo peels and isolation of naringin with antioxidant activities by Sephadex LH20 gel chromatography |
| title_full | Response surface methodology-optimized extraction of flavonoids from pomelo peels and isolation of naringin with antioxidant activities by Sephadex LH20 gel chromatography |
| title_fullStr | Response surface methodology-optimized extraction of flavonoids from pomelo peels and isolation of naringin with antioxidant activities by Sephadex LH20 gel chromatography |
| title_full_unstemmed | Response surface methodology-optimized extraction of flavonoids from pomelo peels and isolation of naringin with antioxidant activities by Sephadex LH20 gel chromatography |
| title_short | Response surface methodology-optimized extraction of flavonoids from pomelo peels and isolation of naringin with antioxidant activities by Sephadex LH20 gel chromatography |
| title_sort | response surface methodology optimized extraction of flavonoids from pomelo peels and isolation of naringin with antioxidant activities by sephadex lh20 gel chromatography |
| topic | Flavonoids Pomelo peels Sephadex LH-20 Response surface methodology Debittering |
| url | http://www.sciencedirect.com/science/article/pii/S2665927123001788 |
| work_keys_str_mv | AT yuchenshangguan responsesurfacemethodologyoptimizedextractionofflavonoidsfrompomelopeelsandisolationofnaringinwithantioxidantactivitiesbysephadexlh20gelchromatography AT jingni responsesurfacemethodologyoptimizedextractionofflavonoidsfrompomelopeelsandisolationofnaringinwithantioxidantactivitiesbysephadexlh20gelchromatography AT lilijiang responsesurfacemethodologyoptimizedextractionofflavonoidsfrompomelopeelsandisolationofnaringinwithantioxidantactivitiesbysephadexlh20gelchromatography AT yanghu responsesurfacemethodologyoptimizedextractionofflavonoidsfrompomelopeelsandisolationofnaringinwithantioxidantactivitiesbysephadexlh20gelchromatography AT chuanbohe responsesurfacemethodologyoptimizedextractionofflavonoidsfrompomelopeelsandisolationofnaringinwithantioxidantactivitiesbysephadexlh20gelchromatography AT yingma responsesurfacemethodologyoptimizedextractionofflavonoidsfrompomelopeelsandisolationofnaringinwithantioxidantactivitiesbysephadexlh20gelchromatography AT guohongwu responsesurfacemethodologyoptimizedextractionofflavonoidsfrompomelopeelsandisolationofnaringinwithantioxidantactivitiesbysephadexlh20gelchromatography AT hejianxiong responsesurfacemethodologyoptimizedextractionofflavonoidsfrompomelopeelsandisolationofnaringinwithantioxidantactivitiesbysephadexlh20gelchromatography |