SOMATIC EMBRYOGENESIS FROM MERISTEM EXPLANTS OF GINGER
The use of planting materials from in vitro culture, especially derived from somatic em -bryos has some advantages such as genetically stable and pathogen-free. Meristem culture of ginger through somatic embryogenesis could be a potential method for producing pathogen-free planting materials. Somat...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
SEAMEO, BIOTROP
2008-01-01
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| Series: | Biotropia: The Southeast Asian Journal of Tropical Biology |
| Subjects: | |
| Online Access: | http://journal.biotrop.org/index.php/biotropia/article/view/2/3 |
| _version_ | 1811192196181786624 |
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| author | OTIH ROSTIANA SITTI FATIMAH SYAHID |
| author_facet | OTIH ROSTIANA SITTI FATIMAH SYAHID |
| author_sort | OTIH ROSTIANA |
| collection | DOAJ |
| description | The use of planting materials from in vitro culture, especially derived from somatic em -bryos has some advantages such as genetically stable and pathogen-free. Meristem culture of ginger through somatic embryogenesis could be a potential method for producing pathogen-free planting materials. Somatic embryogenesis on ginger was performed to obtain vigorous plantlets having the same rhizome size as the mother plant. Callus was induced from meristem tissue of inner bud of Indonesian ginger rhizome Var. Cimanggu-1 and consecutively subcultured into certain media at each steps of experiments. The vigorous embryogenic calli were observed on MS medium containing 100 mgl-1 glutamine and 2% sucrose with addition of 1.0 mgl-1 2,4-D + 3.0 mgl-1 BA. The highest number of somatic embryos (about 82.0.g-1 friable calli) was achieved on that medium, 4 weeks after culturing. Furthermore, the optimum growth of embryogenic calli containing somatic embryo was obtained on MS medium enriched with 6% sucrose. The high-est number of mature somatic embryos (57.2 embryos) was achieved on MS medium, 18 days after incubation. The regeneration potency of somatic embryos obtained from ginger meristem was 51.20%.g-1 friable callus. The valuable result of this study was the achievement of normal rhizome size of regenerated plantlets, instead of micro rhizome. |
| first_indexed | 2024-04-11T23:48:43Z |
| format | Article |
| id | doaj.art-8d1a6b66fd454258a37cb2338299c52e |
| institution | Directory Open Access Journal |
| issn | 0215-6334 1907-770X |
| language | English |
| last_indexed | 2024-04-11T23:48:43Z |
| publishDate | 2008-01-01 |
| publisher | SEAMEO, BIOTROP |
| record_format | Article |
| series | Biotropia: The Southeast Asian Journal of Tropical Biology |
| spelling | doaj.art-8d1a6b66fd454258a37cb2338299c52e2022-12-22T03:56:34ZengSEAMEO, BIOTROPBiotropia: The Southeast Asian Journal of Tropical Biology0215-63341907-770X2008-01-011511224SOMATIC EMBRYOGENESIS FROM MERISTEM EXPLANTS OF GINGEROTIH ROSTIANASITTI FATIMAH SYAHIDThe use of planting materials from in vitro culture, especially derived from somatic em -bryos has some advantages such as genetically stable and pathogen-free. Meristem culture of ginger through somatic embryogenesis could be a potential method for producing pathogen-free planting materials. Somatic embryogenesis on ginger was performed to obtain vigorous plantlets having the same rhizome size as the mother plant. Callus was induced from meristem tissue of inner bud of Indonesian ginger rhizome Var. Cimanggu-1 and consecutively subcultured into certain media at each steps of experiments. The vigorous embryogenic calli were observed on MS medium containing 100 mgl-1 glutamine and 2% sucrose with addition of 1.0 mgl-1 2,4-D + 3.0 mgl-1 BA. The highest number of somatic embryos (about 82.0.g-1 friable calli) was achieved on that medium, 4 weeks after culturing. Furthermore, the optimum growth of embryogenic calli containing somatic embryo was obtained on MS medium enriched with 6% sucrose. The high-est number of mature somatic embryos (57.2 embryos) was achieved on MS medium, 18 days after incubation. The regeneration potency of somatic embryos obtained from ginger meristem was 51.20%.g-1 friable callus. The valuable result of this study was the achievement of normal rhizome size of regenerated plantlets, instead of micro rhizome.http://journal.biotrop.org/index.php/biotropia/article/view/2/3Zingiber officinale Rosc.meristem culturesomatic embryogenesis. |
| spellingShingle | OTIH ROSTIANA SITTI FATIMAH SYAHID SOMATIC EMBRYOGENESIS FROM MERISTEM EXPLANTS OF GINGER Biotropia: The Southeast Asian Journal of Tropical Biology Zingiber officinale Rosc. meristem culture somatic embryogenesis. |
| title | SOMATIC EMBRYOGENESIS FROM MERISTEM EXPLANTS OF GINGER |
| title_full | SOMATIC EMBRYOGENESIS FROM MERISTEM EXPLANTS OF GINGER |
| title_fullStr | SOMATIC EMBRYOGENESIS FROM MERISTEM EXPLANTS OF GINGER |
| title_full_unstemmed | SOMATIC EMBRYOGENESIS FROM MERISTEM EXPLANTS OF GINGER |
| title_short | SOMATIC EMBRYOGENESIS FROM MERISTEM EXPLANTS OF GINGER |
| title_sort | somatic embryogenesis from meristem explants of ginger |
| topic | Zingiber officinale Rosc. meristem culture somatic embryogenesis. |
| url | http://journal.biotrop.org/index.php/biotropia/article/view/2/3 |
| work_keys_str_mv | AT otihrostiana somaticembryogenesisfrommeristemexplantsofginger AT sittifatimahsyahid somaticembryogenesisfrommeristemexplantsofginger |