Color standardization in whole slide imaging using a color calibration slide
Background: Color consistency in histology images is still an issue in digital pathology. Different imaging systems reproduced the colors of a histological slide differently. Materials and Methods: Color correction was implemented using the color information of the nine color patches of a color cali...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2014-01-01
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| Series: | Journal of Pathology Informatics |
| Subjects: | |
| Online Access: | http://www.jpathinformatics.org/article.asp?issn=2153-3539;year=2014;volume=5;issue=1;spage=4;epage=4;aulast=Bautista |
| _version_ | 1818216701217472512 |
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| author | Pinky A Bautista Noriaki Hashimoto Yukako Yagi |
| author_facet | Pinky A Bautista Noriaki Hashimoto Yukako Yagi |
| author_sort | Pinky A Bautista |
| collection | DOAJ |
| description | Background: Color consistency in histology images is still an issue in digital pathology. Different imaging systems reproduced the colors of a histological slide differently. Materials and Methods: Color correction was implemented using the color information of the nine color patches of a color calibration slide. The inherent spectral colors of these patches along with their scanned colors were used to derive a color correction matrix whose coefficients were used to convert the pixels′ colors to their target colors. Results: There was a significant reduction in the CIELAB color difference, between images of the same H & E histological slide produced by two different whole slide scanners by 3.42 units, P < 0.001 at 95% confidence level. Conclusion: Color variations in histological images brought about by whole slide scanning can be effectively normalized with the use of the color calibration slide. |
| first_indexed | 2024-12-12T06:56:09Z |
| format | Article |
| id | doaj.art-8d1cf21c30454ab6a4b6e2121c504f24 |
| institution | Directory Open Access Journal |
| issn | 2153-3539 |
| language | English |
| last_indexed | 2024-12-12T06:56:09Z |
| publishDate | 2014-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pathology Informatics |
| spelling | doaj.art-8d1cf21c30454ab6a4b6e2121c504f242022-12-22T00:33:57ZengElsevierJournal of Pathology Informatics2153-35392014-01-01514410.4103/2153-3539.126153Color standardization in whole slide imaging using a color calibration slidePinky A BautistaNoriaki HashimotoYukako YagiBackground: Color consistency in histology images is still an issue in digital pathology. Different imaging systems reproduced the colors of a histological slide differently. Materials and Methods: Color correction was implemented using the color information of the nine color patches of a color calibration slide. The inherent spectral colors of these patches along with their scanned colors were used to derive a color correction matrix whose coefficients were used to convert the pixels′ colors to their target colors. Results: There was a significant reduction in the CIELAB color difference, between images of the same H & E histological slide produced by two different whole slide scanners by 3.42 units, P < 0.001 at 95% confidence level. Conclusion: Color variations in histological images brought about by whole slide scanning can be effectively normalized with the use of the color calibration slide.http://www.jpathinformatics.org/article.asp?issn=2153-3539;year=2014;volume=5;issue=1;spage=4;epage=4;aulast=BautistaColor calibration, color correction, color normalization, color standardization, whole slide image, whole slide scanning |
| spellingShingle | Pinky A Bautista Noriaki Hashimoto Yukako Yagi Color standardization in whole slide imaging using a color calibration slide Journal of Pathology Informatics Color calibration, color correction, color normalization, color standardization, whole slide image, whole slide scanning |
| title | Color standardization in whole slide imaging using a color calibration slide |
| title_full | Color standardization in whole slide imaging using a color calibration slide |
| title_fullStr | Color standardization in whole slide imaging using a color calibration slide |
| title_full_unstemmed | Color standardization in whole slide imaging using a color calibration slide |
| title_short | Color standardization in whole slide imaging using a color calibration slide |
| title_sort | color standardization in whole slide imaging using a color calibration slide |
| topic | Color calibration, color correction, color normalization, color standardization, whole slide image, whole slide scanning |
| url | http://www.jpathinformatics.org/article.asp?issn=2153-3539;year=2014;volume=5;issue=1;spage=4;epage=4;aulast=Bautista |
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