Activation of long-non-coding RNA NEAT1 sponging microRNA-147 inhibits radiation damage by targeting PDPK1 in troxerutin radioprotection
Summary: A better understanding of the molecular mechanism involving the lncRNA-miRNA-mRNA network underlying radiation damage can be beneficial for radioprotection. This study was designed to investigate the potential role of lncRNA NEAT1, miR-147 and Phosphoinositide Dependent Protein Kinase 1 (PD...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-02-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223000093 |
| _version_ | 1828012390144278528 |
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| author | Yong-jian Hu Gui-yuan Song Fan Zhang Nan Zhang Fei Wang Jing-long Wang Xia Wang Tao-yang Wang Yu-feng Li Yi-di Yan Wen-tao Dou Chen-yi Cheng Ping Xu |
| author_facet | Yong-jian Hu Gui-yuan Song Fan Zhang Nan Zhang Fei Wang Jing-long Wang Xia Wang Tao-yang Wang Yu-feng Li Yi-di Yan Wen-tao Dou Chen-yi Cheng Ping Xu |
| author_sort | Yong-jian Hu |
| collection | DOAJ |
| description | Summary: A better understanding of the molecular mechanism involving the lncRNA-miRNA-mRNA network underlying radiation damage can be beneficial for radioprotection. This study was designed to investigate the potential role of lncRNA NEAT1, miR-147 and Phosphoinositide Dependent Protein Kinase 1 (PDPK1) interaction in radioprotection by troxerutin (TRT). We first demonstrated that NEAT1 sponged miR-147, and PDPK1 mRNA was the primary target of miR-147. In the cells, the NEAT1 and PDPK1 levels were downregulated after the radiation but increased after the treatment with TRT. The miR-147 level was significantly induced by radiation and inhibited by TRT. NEAT1 negatively regulated the expression of miR-147, whereas miR-47 targeted PDPK1 to downregulate its expression. In radioprotection, TRT effectively upregulated NEAT1 to inhibit miR-147 and to upregulate PDPK1. We concluded that TRT could promote radioprotection by stimulating NEAT1 to upregulate PDPK1 expression by suppressing miR-147. NEAT1 could be a critical therapeutic target of radiation damage. |
| first_indexed | 2024-04-10T09:32:07Z |
| format | Article |
| id | doaj.art-8d20c0b548024756baa467da7986a3db |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-10T09:32:07Z |
| publishDate | 2023-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-8d20c0b548024756baa467da7986a3db2023-02-19T04:26:36ZengElsevieriScience2589-00422023-02-01262105932Activation of long-non-coding RNA NEAT1 sponging microRNA-147 inhibits radiation damage by targeting PDPK1 in troxerutin radioprotectionYong-jian Hu0Gui-yuan Song1Fan Zhang2Nan Zhang3Fei Wang4Jing-long Wang5Xia Wang6Tao-yang Wang7Yu-feng Li8Yi-di Yan9Wen-tao Dou10Chen-yi Cheng11Ping Xu12School of Food and Biomedicine, Zaozhuang University, Zaozhuang, Shandong 277160, China; Henan Key Laboratory of Medical Tissue Regeneration, Xinxiang Medical University, Xinxiang, Henan 453003, ChinaSchool of Food and Biomedicine, Zaozhuang University, Zaozhuang, Shandong 277160, China; School of Public Health, Weifang Medical University, Weifang, Shandong 261000, China; Radiology Laboratory, Central Laboratory, Rizhao People’s Hospital, Rizhao, Shandong 276800, ChinaSchool of Food and Biomedicine, Zaozhuang University, Zaozhuang, Shandong 277160, China; Henan Key Laboratory of Medical Tissue Regeneration, Xinxiang Medical University, Xinxiang, Henan 453003, ChinaSchool of Food and Biomedicine, Zaozhuang University, Zaozhuang, Shandong 277160, ChinaSchool of Food and Biomedicine, Zaozhuang University, Zaozhuang, Shandong 277160, ChinaSchool of Food and Biomedicine, Zaozhuang University, Zaozhuang, Shandong 277160, ChinaCollege of Medical Laboratory, Xinxiang Medical University, Xinxiang, Henan 453003, ChinaSchool of Food and Biomedicine, Zaozhuang University, Zaozhuang, Shandong 277160, China; Henan Key Laboratory of Medical Tissue Regeneration, Xinxiang Medical University, Xinxiang, Henan 453003, ChinaRadiology Laboratory, Central Laboratory, Rizhao People’s Hospital, Rizhao, Shandong 276800, ChinaBasic Medical School, Xinxiang Medical University, Xinxiang, Henan 453003, ChinaBasic Medical School, Xinxiang Medical University, Xinxiang, Henan 453003, ChinaBasic Medical School, Xinxiang Medical University, Xinxiang, Henan 453003, ChinaSchool of Food and Biomedicine, Zaozhuang University, Zaozhuang, Shandong 277160, China; Henan Key Laboratory of Medical Tissue Regeneration, Xinxiang Medical University, Xinxiang, Henan 453003, China; Corresponding authorSummary: A better understanding of the molecular mechanism involving the lncRNA-miRNA-mRNA network underlying radiation damage can be beneficial for radioprotection. This study was designed to investigate the potential role of lncRNA NEAT1, miR-147 and Phosphoinositide Dependent Protein Kinase 1 (PDPK1) interaction in radioprotection by troxerutin (TRT). We first demonstrated that NEAT1 sponged miR-147, and PDPK1 mRNA was the primary target of miR-147. In the cells, the NEAT1 and PDPK1 levels were downregulated after the radiation but increased after the treatment with TRT. The miR-147 level was significantly induced by radiation and inhibited by TRT. NEAT1 negatively regulated the expression of miR-147, whereas miR-47 targeted PDPK1 to downregulate its expression. In radioprotection, TRT effectively upregulated NEAT1 to inhibit miR-147 and to upregulate PDPK1. We concluded that TRT could promote radioprotection by stimulating NEAT1 to upregulate PDPK1 expression by suppressing miR-147. NEAT1 could be a critical therapeutic target of radiation damage.http://www.sciencedirect.com/science/article/pii/S2589004223000093Biological sciencesCell biologyMolecular biologyMolecular mechanism of gene regulation |
| spellingShingle | Yong-jian Hu Gui-yuan Song Fan Zhang Nan Zhang Fei Wang Jing-long Wang Xia Wang Tao-yang Wang Yu-feng Li Yi-di Yan Wen-tao Dou Chen-yi Cheng Ping Xu Activation of long-non-coding RNA NEAT1 sponging microRNA-147 inhibits radiation damage by targeting PDPK1 in troxerutin radioprotection iScience Biological sciences Cell biology Molecular biology Molecular mechanism of gene regulation |
| title | Activation of long-non-coding RNA NEAT1 sponging microRNA-147 inhibits radiation damage by targeting PDPK1 in troxerutin radioprotection |
| title_full | Activation of long-non-coding RNA NEAT1 sponging microRNA-147 inhibits radiation damage by targeting PDPK1 in troxerutin radioprotection |
| title_fullStr | Activation of long-non-coding RNA NEAT1 sponging microRNA-147 inhibits radiation damage by targeting PDPK1 in troxerutin radioprotection |
| title_full_unstemmed | Activation of long-non-coding RNA NEAT1 sponging microRNA-147 inhibits radiation damage by targeting PDPK1 in troxerutin radioprotection |
| title_short | Activation of long-non-coding RNA NEAT1 sponging microRNA-147 inhibits radiation damage by targeting PDPK1 in troxerutin radioprotection |
| title_sort | activation of long non coding rna neat1 sponging microrna 147 inhibits radiation damage by targeting pdpk1 in troxerutin radioprotection |
| topic | Biological sciences Cell biology Molecular biology Molecular mechanism of gene regulation |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223000093 |
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