EMT Transcription Factors Are Involved in the Altered Cell Adhesion under Simulated Microgravity Effect or Overloading by Regulation of E-cadherin
In order to study the effect of stress changes on cell adhesion, HUVEC, and MCF-7 cells were treated with simulated microgravity effect (SMG) and overloading (OL). Methods: Rotating Wall Vessel (2D-RWVS) bioreactor was used to create different culture conditions. In addition, the alteration of cell...
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| Format: | Article |
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MDPI AG
2020-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/4/1349 |
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| author | Shuliang Shi Qiao Li Qiuying Cao Yan Diao Yao Zhang Lei Yue Lijun Wei |
| author_facet | Shuliang Shi Qiao Li Qiuying Cao Yan Diao Yao Zhang Lei Yue Lijun Wei |
| author_sort | Shuliang Shi |
| collection | DOAJ |
| description | In order to study the effect of stress changes on cell adhesion, HUVEC, and MCF-7 cells were treated with simulated microgravity effect (SMG) and overloading (OL). Methods: Rotating Wall Vessel (2D-RWVS) bioreactor was used to create different culture conditions. In addition, the alteration of cell adhesion states, adhesion proteins, and relating factors of adhesion molecules under these two conditions were detected using cell adhesion assay, immunofluorescence, western blot, and qRT-PCR technology. Results: The results showed that the adhesion of cells decreased under SMG, while increased under OL. The expressions of integrin β1, paxillin, and E-cadherin under SMG condition were down-regulated as compared to that of the control group showing a time-dependent pattern of the decreasing. However, under OL condition, the expressions of adhesion proteins were up-regulated as compared to that of the control group, with a time-dependent pattern of increasing. EMT transcription factors Snail, twist, and ZEB1 were up-regulated under SMG while down-regulated under OL. Conclusion: Collectively our results indicated that cells could respond to stress changes to regulate the expressions of adhesion proteins and adapt their adhesion state to the altered mechanical environment. The altered cell adhesion in response to the mechanical stress may involve the changed expression of EMT-inducing factors, Snail, Twist, and ZEB1under the SMG/OL conditions. |
| first_indexed | 2024-04-12T01:48:29Z |
| format | Article |
| id | doaj.art-8d27200697cc4b13b0fe3a44181e75c1 |
| institution | Directory Open Access Journal |
| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-12T01:48:29Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-8d27200697cc4b13b0fe3a44181e75c12022-12-22T03:53:01ZengMDPI AGInternational Journal of Molecular Sciences1422-00672020-02-01214134910.3390/ijms21041349ijms21041349EMT Transcription Factors Are Involved in the Altered Cell Adhesion under Simulated Microgravity Effect or Overloading by Regulation of E-cadherinShuliang Shi0Qiao Li1Qiuying Cao2Yan Diao3Yao Zhang4Lei Yue5Lijun Wei6School of Life Science and Technology, Harbin Institute of Technology, Harbin 150001, ChinaSchool of Life Science and Technology, Harbin Institute of Technology, Harbin 150001, ChinaSchool of Life Science and Technology, Harbin Institute of Technology, Harbin 150001, ChinaCollege of Life Science, China West Normal University, Nanchong 637002, ChinaSchool of Life Science and Technology, Harbin Institute of Technology, Harbin 150001, ChinaSchool of Life Science and Technology, Harbin Institute of Technology, Harbin 150001, ChinaSchool of Life Science and Technology, Harbin Institute of Technology, Harbin 150001, ChinaIn order to study the effect of stress changes on cell adhesion, HUVEC, and MCF-7 cells were treated with simulated microgravity effect (SMG) and overloading (OL). Methods: Rotating Wall Vessel (2D-RWVS) bioreactor was used to create different culture conditions. In addition, the alteration of cell adhesion states, adhesion proteins, and relating factors of adhesion molecules under these two conditions were detected using cell adhesion assay, immunofluorescence, western blot, and qRT-PCR technology. Results: The results showed that the adhesion of cells decreased under SMG, while increased under OL. The expressions of integrin β1, paxillin, and E-cadherin under SMG condition were down-regulated as compared to that of the control group showing a time-dependent pattern of the decreasing. However, under OL condition, the expressions of adhesion proteins were up-regulated as compared to that of the control group, with a time-dependent pattern of increasing. EMT transcription factors Snail, twist, and ZEB1 were up-regulated under SMG while down-regulated under OL. Conclusion: Collectively our results indicated that cells could respond to stress changes to regulate the expressions of adhesion proteins and adapt their adhesion state to the altered mechanical environment. The altered cell adhesion in response to the mechanical stress may involve the changed expression of EMT-inducing factors, Snail, Twist, and ZEB1under the SMG/OL conditions.https://www.mdpi.com/1422-0067/21/4/1349simulated microgravity effect (smg)mechanical overloading (ol)cell adhesionhuvec and mcf-7 cell |
| spellingShingle | Shuliang Shi Qiao Li Qiuying Cao Yan Diao Yao Zhang Lei Yue Lijun Wei EMT Transcription Factors Are Involved in the Altered Cell Adhesion under Simulated Microgravity Effect or Overloading by Regulation of E-cadherin International Journal of Molecular Sciences simulated microgravity effect (smg) mechanical overloading (ol) cell adhesion huvec and mcf-7 cell |
| title | EMT Transcription Factors Are Involved in the Altered Cell Adhesion under Simulated Microgravity Effect or Overloading by Regulation of E-cadherin |
| title_full | EMT Transcription Factors Are Involved in the Altered Cell Adhesion under Simulated Microgravity Effect or Overloading by Regulation of E-cadherin |
| title_fullStr | EMT Transcription Factors Are Involved in the Altered Cell Adhesion under Simulated Microgravity Effect or Overloading by Regulation of E-cadherin |
| title_full_unstemmed | EMT Transcription Factors Are Involved in the Altered Cell Adhesion under Simulated Microgravity Effect or Overloading by Regulation of E-cadherin |
| title_short | EMT Transcription Factors Are Involved in the Altered Cell Adhesion under Simulated Microgravity Effect or Overloading by Regulation of E-cadherin |
| title_sort | emt transcription factors are involved in the altered cell adhesion under simulated microgravity effect or overloading by regulation of e cadherin |
| topic | simulated microgravity effect (smg) mechanical overloading (ol) cell adhesion huvec and mcf-7 cell |
| url | https://www.mdpi.com/1422-0067/21/4/1349 |
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