Effects of the Blending Ratio on the Design of Keratin/Poly(butylene succinate) Nanofibers for Drug Delivery Applications
In recent years there has been a growing interest in the use of proteins as biocompatible and environmentally friendly biomolecules for the design of wound healing and drug delivery systems. Keratin is a fascinating protein, obtainable from several keratinous biomasses such as wool, hair or nails, w...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-08-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/11/8/1194 |
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| author | Giulia Guidotti Michelina Soccio Edoardo Bondi Tamara Posati Giovanna Sotgiu Roberto Zamboni Armida Torreggiani Franco Corticelli Nadia Lotti Annalisa Aluigi |
| author_facet | Giulia Guidotti Michelina Soccio Edoardo Bondi Tamara Posati Giovanna Sotgiu Roberto Zamboni Armida Torreggiani Franco Corticelli Nadia Lotti Annalisa Aluigi |
| author_sort | Giulia Guidotti |
| collection | DOAJ |
| description | In recent years there has been a growing interest in the use of proteins as biocompatible and environmentally friendly biomolecules for the design of wound healing and drug delivery systems. Keratin is a fascinating protein, obtainable from several keratinous biomasses such as wool, hair or nails, with intrinsic bioactive properties including stimulatory effects on wound repair and excellent carrier capability. In this work keratin/poly(butylene succinate) blend solutions with functional properties tunable by manipulating the polymer blending ratios were prepared by using 1,1,1,3,3,3-hexafluoroisopropanol as common solvent. Afterwards, these solutions doped with rhodamine B (RhB), were electrospun into blend mats and the drug release mechanism and kinetics as a function of blend composition was studied, in order to understand the potential of such membranes as drug delivery systems. The electrophoresis analysis carried out on keratin revealed that the solvent used does not degrade the protein. Moreover, all the blend solutions showed a non-Newtonian behavior, among which the Keratin/PBS 70/30 and 30/70 ones showed an amplified orientation ability of the polymer chains when subjected to a shear stress. Therefore, the resulting nanofibers showed thinner mean diameters and narrower diameter distributions compared to the Keratin/PBS 50/50 blend solution. The thermal stability and the mechanical properties of the blend electrospun mats improved by increasing the PBS content. Finally, the RhB release rate increased by increasing the keratin content of the mats and the drug diffused as drug-protein complex. |
| first_indexed | 2024-03-10T08:58:35Z |
| format | Article |
| id | doaj.art-8d27fb7c4918417ca6fc039be84647d7 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T08:58:35Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-8d27fb7c4918417ca6fc039be84647d72023-11-22T06:56:10ZengMDPI AGBiomolecules2218-273X2021-08-01118119410.3390/biom11081194Effects of the Blending Ratio on the Design of Keratin/Poly(butylene succinate) Nanofibers for Drug Delivery ApplicationsGiulia Guidotti0Michelina Soccio1Edoardo Bondi2Tamara Posati3Giovanna Sotgiu4Roberto Zamboni5Armida Torreggiani6Franco Corticelli7Nadia Lotti8Annalisa Aluigi9Department of Civil, Chemical, Environmental, and Materials Engineering, University of Bologna, Via Terracini 28, 40131 Bologna, ItalyDepartment of Civil, Chemical, Environmental, and Materials Engineering, University of Bologna, Via Terracini 28, 40131 Bologna, ItalyDepartment of Civil, Chemical, Environmental, and Materials Engineering, University of Bologna, Via Terracini 28, 40131 Bologna, ItalyInstitute of Organic Synthesis and Photoreactivity, Italian National Research Council, Via P. Gobetti 101, 40129 Bologna, ItalyInstitute of Organic Synthesis and Photoreactivity, Italian National Research Council, Via P. Gobetti 101, 40129 Bologna, ItalyInstitute of Organic Synthesis and Photoreactivity, Italian National Research Council, Via P. Gobetti 101, 40129 Bologna, ItalyInstitute of Organic Synthesis and Photoreactivity, Italian National Research Council, Via P. Gobetti 101, 40129 Bologna, ItalyInstitute for Microelectronics and Microsystems, National Research Council, Via P. Gobetti 101, 40129 Bologna, ItalyDepartment of Civil, Chemical, Environmental, and Materials Engineering, University of Bologna, Via Terracini 28, 40131 Bologna, ItalyInstitute of Organic Synthesis and Photoreactivity, Italian National Research Council, Via P. Gobetti 101, 40129 Bologna, ItalyIn recent years there has been a growing interest in the use of proteins as biocompatible and environmentally friendly biomolecules for the design of wound healing and drug delivery systems. Keratin is a fascinating protein, obtainable from several keratinous biomasses such as wool, hair or nails, with intrinsic bioactive properties including stimulatory effects on wound repair and excellent carrier capability. In this work keratin/poly(butylene succinate) blend solutions with functional properties tunable by manipulating the polymer blending ratios were prepared by using 1,1,1,3,3,3-hexafluoroisopropanol as common solvent. Afterwards, these solutions doped with rhodamine B (RhB), were electrospun into blend mats and the drug release mechanism and kinetics as a function of blend composition was studied, in order to understand the potential of such membranes as drug delivery systems. The electrophoresis analysis carried out on keratin revealed that the solvent used does not degrade the protein. Moreover, all the blend solutions showed a non-Newtonian behavior, among which the Keratin/PBS 70/30 and 30/70 ones showed an amplified orientation ability of the polymer chains when subjected to a shear stress. Therefore, the resulting nanofibers showed thinner mean diameters and narrower diameter distributions compared to the Keratin/PBS 50/50 blend solution. The thermal stability and the mechanical properties of the blend electrospun mats improved by increasing the PBS content. Finally, the RhB release rate increased by increasing the keratin content of the mats and the drug diffused as drug-protein complex.https://www.mdpi.com/2218-273X/11/8/1194keratinpoly(butylene succinate)electrospinningdrug release |
| spellingShingle | Giulia Guidotti Michelina Soccio Edoardo Bondi Tamara Posati Giovanna Sotgiu Roberto Zamboni Armida Torreggiani Franco Corticelli Nadia Lotti Annalisa Aluigi Effects of the Blending Ratio on the Design of Keratin/Poly(butylene succinate) Nanofibers for Drug Delivery Applications Biomolecules keratin poly(butylene succinate) electrospinning drug release |
| title | Effects of the Blending Ratio on the Design of Keratin/Poly(butylene succinate) Nanofibers for Drug Delivery Applications |
| title_full | Effects of the Blending Ratio on the Design of Keratin/Poly(butylene succinate) Nanofibers for Drug Delivery Applications |
| title_fullStr | Effects of the Blending Ratio on the Design of Keratin/Poly(butylene succinate) Nanofibers for Drug Delivery Applications |
| title_full_unstemmed | Effects of the Blending Ratio on the Design of Keratin/Poly(butylene succinate) Nanofibers for Drug Delivery Applications |
| title_short | Effects of the Blending Ratio on the Design of Keratin/Poly(butylene succinate) Nanofibers for Drug Delivery Applications |
| title_sort | effects of the blending ratio on the design of keratin poly butylene succinate nanofibers for drug delivery applications |
| topic | keratin poly(butylene succinate) electrospinning drug release |
| url | https://www.mdpi.com/2218-273X/11/8/1194 |
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